34 results about "Glutathione peroxidase activity" patented technology

The invention belongs to the field of traditional Chinese medicine preparation, relating to application of monomer gentiamarin extracted in the traditional Chinese medicine in preparing medicine for preventing and treating acute pancreatitis. In the invention, a model which is recognized by academic circles at home and abroad and is formed by continuously injecting caerulein to cause the acute pancreatitis of mice in enterocoelia and carrying out bezoar sodium taurocholate retrograde injection in cholangiopan so as to cause the acute pancreatitis of a rat, is adopted to study the action of the gentiamarin. The experiment result discloses that the gentiamarin has obvious function of resisting the acute pancreatitis caused by various reasons, and the action mechanism for resisting the acute pancreatitis is related to the releasing of the inflammatory factor, reduction of concentration of the tumor necrosis factor-alpha and interleukin-1beta in the serum or tissue, the increasing of the glutathione peroxidase activity in the serum or tissue, and the clearing of the radical and the expression of NF-kB P65.

Production of transparent acid containing tellurium with high glutathione oxide enzyme activity is artificial enzyme process with GPX activity. It consists of transparent acid activating, tellurium sodium hydride preparing, transparent acid tellurium reacting, tellurium transparent acid oxidizing and after processing. The production is carried out by activating transparent acid with tolylsulfonyl chlorin, preparing transparent acid benzene sulfonyl ester, dialyzing in distilled water, adding tellurium powder and sodium borohydride into anhydrous alcohol, heating and reverse flowing in nitrogen system, adding anaerobic glacial acetic acid, preparing alcohol solution of tellurium sodium hydride preparing, alcohol of tellurium sodium hydride reacting with transparent acid benzene sulfonyl ester solution, obtaining transparent acid containing the, the reacting system expose in air, centrifugal oxidizing, alcohol settling, and washing. It achieves simple process, high output, higher resistance to oxidation and GPX activity.

The invention relates to the technical filed of food fermentation, and specifically relates to a preparation method of dark soy sauce with an anti-oxidation function. The dark soy sauce with the anti-oxidation function is prepared from the following raw materials: cordyceps militaris, soy sauce koji, oat, needle mushrooms, Chinese wolfberry fruits, hypsizygus marmoreus, bearded tooth mushrooms, a taste enhancer, crystal sugar, a sweetener, salt, and water. The preparation method of the dark soy sauce with the anti-oxidation function comprises the following steps of: preparing soup, preparing original soy sauce liquid, carrying out blending, carrying out concentrating, and carrying out sun-curing so as to obtain soy sauce liquid; and then, blending the sun-cured soy sauce liquid with the sweetener, the taste enhancer and the like, and carrying out cooking, filtering and filling so as to obtain the dark soy sauce. The dark soy sauce prepared by the preparation method is capable of improving total anti-oxidation capacity, superoxide dismutase activity and glutathione peroxidase activity of the serum and the liver, decreasing malondialdehyde content of the serum and the liver, reducing monoamine oxidase activity of the whole brain, improving peroxidase activity of the heart, and increasing immunoglobulin G content and immunoglobulin M content so as to achieve the functions of scavenging free radical, reduce heart oxidation and enhancing body immunity.

The invention provides a Chinese herbal medicine additive capable of improving the oxidation resistance of a parent body during the milking stage and an application method thereof. Astragalus root is dried, is crushed, and then is evenly added into the feedstuff for milking rats and sows and the extender of concentrated feedstuff for milking cows according to the amount of 1 percent. The medicine additive adopts a technical proposal that the rats are taken as model animals; the Chinese herbal medicine with a stable antioxidation effect is selected from a plurality of milk promoting Chinese herbal medicines; and then tests on the rats, sows and cows are carried out. Finally, 1 percent of astragalus root added into the feedstuff for milking rats and sows and the extender of concentrated feedstuff for milking cows is found to have the best antioxidation effect. The Chinese herbal medicine additive improves the activity of superoxide dismutase and glutathione peroxidase in the blood serum and cardiac muscle and the general oxidation resistance level for the rats, sows and cows in the medium and later milking stage, and remarkably reduces the level of malonaldehyde in the blood serum, liver, kidney and cardiac muscle in the later milking stage (P<0.05 or P<0.01). The Chinese herbal medicine additive can improve the health condition of mammals after application, thereby bringing about good economical and ecological benefits.

The invention discloses a lactobacillus fermentum (Lactobacillus fermentum) JX306 with anti-oxidation function and its application, belonging to the technical field of bioengineering. The bacteria has strong DPPH free radical and hydroxyl free radical scavenging ability, high reducing power, strong Fe 2+ Chelating ability and good anti-lipid peroxidation ability, and has good bile salt resistance ability, gastrointestinal fluid resistance ability and hydrogen peroxide tolerance; it can also significantly increase glutathione peroxidase activity, reduce Malondialdehyde levels can significantly up-regulate the levels of five antioxidant enzymes including TR3, Prdx1, Gsr, Gpx1, and nuclear transcription factor Nrf2, and can significantly alleviate the histopathological damage induced by D-gal. The Lactobacillus fermentum JX306 of the invention has a great effect on improving the antioxidant performance of food as a fermented strain, and has wide application prospects.

Production of transparent acid containing tellurium with high glutathione oxide enzyme activity is artificial enzyme process with GPX activity. It consists of transparent acid activating, tellurium sodium hydride preparing, transparent acid tellurium reacting, tellurium transparent acid oxidizing and after processing. The production is carried out by activating transparent acid with tolylsulfonyl chlorin, preparing transparent acid benzene sulfonyl ester, dialyzing in distilled water, adding tellurium powder and sodium borohydride into anhydrous alcohol, heating and reverse flowing in nitrogen system, adding anaerobic glacial acetic acid, preparing alcohol solution of tellurium sodium hydride preparing, alcohol of tellurium sodium hydride reacting with transparent acid benzene sulfonyl ester solution, obtaining transparent acid containing the, the reacting system expose in air, centrifugal oxidizing, alcohol settling, and washing. It achieves simple process, high output, higher resistance to oxidation and GPX activity.

The invention provides an application of rosa roxburghii tratt fruit polysaccharides in preparation for blood glucose-reducing medicines, heath products or foods. Researches on the application provided by the invention find that the rosa roxburghii tratt fruit polysaccharides have a good blood glucose-reducing function, can increase an insulin amount, and have a regulation effect on the activities of the corresponding ROS metabolism-related enzymes, that is, the activities of superoxide dismutase, catalase, and glutathion peroxidase to a certain extent. Meanwhile, the application provided by the invention further finds that the effect of a rosa roxburghii tratt fruit high-dose group is equal to the effect of glibenclamide, although glibenclamide is a first-line medicine for treating diabetes, glibenclamide finally causes the hypoglycaemia and kidney diseases of patients, and even causes death in case of being greatly taken for a long time; conversely, rosa roxburghii tratt fruits are edible berries, good in safety, and more suitable for being used as foods, health products or medicines for treating and preventing diabetes.

The invention provides tangut barberry bark fruit polysaccharide which is an extract from a Berberis dasystachya Maxim. fruit; the polysaccharide content of the extract is 60-95% w / w. Researches show that tangut barberry bark fruit polysaccharide has a favorable blood glucose reducing function, can increase the insulin amount, and has a certain adjusting effect on the activities of corresponding ROS metabolism related enzymes such as superoxide dismutase, catalase and glutathione peroxidase. Besides, the effect of the high dose group of a tangut barberry bark fruit is equivalent to that of glibenclamide, while though glibenclamide is a chemical drug for treating diabetes, taking of a large amount of glibenclamide for a long time can cause hypoglycemia and nephropathy finally, or even death; otherwise, the tangut barberry bark fruit is an edible berry, is favorable in safety, and is more suitable for being used as a food, a health product or a drug for treating and preventing diabetes.

A process for preparing selenopapain from papain and sodium selenohydride through dissolving papain in phosphate solution, adding excessive sodium selenohydride, seleno substitution under protection of N2, freeze drying, dissolving in phosphate solution centrifugal removal of Se, removing salt, collecting protein peaks, and freeze drying. Its advantages are simple process high activity of glutathion peroxidase, and excellent antioxidizing power.