34 results about "Glutathione peroxidase activity" patented technology

A cyclodextrin derivative for preventing and treating cardiovascular and cerebrovascular diseases, diabetes, liver injury, diseases in respiratory system, rheumatic and rheumatoid diseases, dermatopathy and tumor is disclosed. It is an artificial analogue enzyme with the activity of glutathion peroxidase.

The invention relates to preparation and application methods of penaeus monodon fermented feed. The preparation method comprises the following steps: respectively carrying out enlarged culture on bacillus licheniformis, lactobacillus plantarum and candida utilis, then mixing in a volume ratio of 1:1:1, adding brown sugar (2g / 1000mL) and running water into the mixed bacterial solution under the condition that the ratio of the culture solution to water is 1:80, uniformly mixing, uniformly stirring with penaeus monodon compound feed in a ratio of 2:5, sealing with a plastic film, and fermenting for 18-28 hours, so that the fermented feed is obtained. The invention also provides the application method of the fermented feed. The prepared fermented feed is used for feeding penaeus monodon once a day, and the feeding amount is 20-30% of the daily total feeding amount. The prepared fermented feed can obviously improve nitric oxide synthase activity, superoxide dismutase activity, catalase activity, phenoloxidase activity, glutathione peroxidase activity and total antioxidant capacity of penaeus monodon and obviously reduce the malondialdehyde content; and composition of florae in intestines of the penaeus monodon is obviously improved, and the survival rate of penaeus monodon culture is obviously increased.

The present invention is one kind of health care medicine with functions of delaying senility and regulating immunity. It incldues deer bone powder and some other components. Animal test shows that the medicine can reduce mouse's myocardial lipofuscin content, raise mouse's glutathione peroxidase activity and serum SOD activity; prolong fruit fly's life; and raise mouse's carbon clearance index, macrophage phagocytizing rate and phagocytizing index.

The invention relates to composite selenium fertilizer for tobacco leaf surface spraying and an application in selenium-enriched tobacco leaf production. Th composite selenium fertilizer comprises thefollowing components, by weight: 7-13 parts of sodium selenite, 10-15 parts of a surfactant, 7-13 parts of diethyl aminoethyl hexanoate, and 240-260 parts of fulvic acid. Compared with direct spraying of a sodium selenite solution, the composite selenium fertilizer for tobacco leaf surface spraying can effectively improve the economic properties of tobacco to significantly increase the proportionof superior tobacco, can maximize the improvement of physical properties of the tobacco to increase the usability, can increase the potassium content of the tobacco to make the chemical composition more coordinated, can increase the content of neophytadiene to increase the concentration of aroma and improve the quality of aroma, and can significantly reduce the content of malondialdehyde and increase the activity of glutathion peroxidase to effectively improve the antioxidant capacity of the tobacco. Compared with conventional selenium fertilizer, the composite selenium fertilizer is improvedin the improvement of the growth of the tobacco and the quality of the tobacco, and can be effectively applied to a production process of selenium-enriched tobacco leaves.

The invention provides a preparation method of chlorella antioxidant polypeptide, which is characterized in that the chlorella protein is extracted by high-pressure homogenization method combined withcellulase / pectinase enzymatic hydrolysis, enzymatic hydrolysis of a specific protease hydrolysate and ultrafiltration membrane separation to obtain chlorella enzymatic hydrolytic peptide with different molecular weight intervals. The molecular weight range of the present invention after enzymatic hydrolysis by a specific protease hydrolysate is 0-3.5 KDa chlorella polypeptides have better scavenging activity of DPPH and hydroxyl radical in vitro than other protease hydrolysates and other chlorella polypeptides with other molecular weight intervals. The ability of scavenging ROS clusters of hepatocellular carcinoma cells in vitro, the inhibition of MDA formation in vivo and the promotion of GSH-Px activity in vivo are more effective than those in vivo. A Chlorella active peptide with bet antioxidant function is prepared from enzyme hydrolysate of specific protease, which is beneficial to that development and utilization of health food and pharmaceutical product with antioxidant function.

The invention discloses lactobacillus fermentum JX306 with an antioxidant function and application thereof, and belongs to the technical field of bioengineering. The lactobacillus fermentum JX306 hasa high DPPH free radical and hydroxyl radical scavenging ability, high reducing power, a high Fe2+ chelating ability, a favorable anti-lipid peroxidation ability, and favorable bile salt tolerance, gastrointestinal fluid resistivity and hydrogen peroxide tolerance; and the glutathione peroxidase activity can be remarkably improved, the malondialdehyde level is reduced, the levels of five antioxidant enzymes such as TR3, Prdx1, Gsr, Gpx1 and a nuclear transcription factor Nrf2 are remarkably improved, and the D-gal induced histopathological lesion can be remarkably relieved. The lactobacillus fermentum JX306 provided by the invention plays a big role on improving the oxidation resistance of food with the lactobacillus fermentum JX306 as a fermenting microbe, and has a wide application prospect.

The present invention discloses a taurine compound preparation for groupers and an application thereof. The taurine compound preparation is composed of the following raw materials in parts by weight: 30-70 parts of taurine, 10-40 parts of alanyl-glutamine, 2-6 parts of vitamin C polyphosphate, 1-5 parts of vitamin E and 4-19 parts of corn starch. The taurine compound preparation for the groupers has the advantages of promoting the growth and feed conversion efficiency of the groupers, reducing the ratio of liver weight and body weight, reducing the deposition of body fat and liver fat of the groupers, can significantly improve the activity and total antioxidant capacity of superoxide dismutase, catalase, serum antioxidant enzymes and glutathione peroxidase in liver and plasma of saddletail groupers, and at the same time significantly reduces the malondialdehyde content.

The invention discloses a production method of selenium-rich hericium erinaceus powder. The production method comprises the steps: (1) culture of liquid strains, (2) homogenizing of strain liquid, (3)solid fermentation culture, and (4) preparation of the selenium-rich hericium erinaceus powder. The selenium-rich hericium erinaceus powder disclosed by the invention can be applied as a selenium nutrition enhancer. The range of the selenium content of the obtained selenium-rich hericium erinaceus powder reaches the Chinese Standard provisions, the ratio of organic selenium is greater than 99.5%,and the selenium in the selenium-rich hericium erinaceus powder produced by the method disclosed by the invention is absorbable and can obviously improve the activity of glutathione peroxidase in mice. The strains in the production method are matched with a liquid strain production process, and the fruiting time is shortened to about 20 days. The product obtained by the production method has a high selenium content and can be applied to milk beverages, rice and products thereof, wheat flour and products thereof, coarse grain flour and products thereof, bread, biscuits and other conventional foods as a nutrition enhancer, so the scope of application is widened and popularization of selenium supplement in national people is effectively realized by virtue of the selenium nutrition enhancer.

The invention provides a benzisoselenazolone quinoline derivative as shown in a formula I, or pharmaceutically acceptable salts, hydrate, solvates, crystal-form isomers or diastereoisomers thereof, as well as an application of compounds or compositions in medicines for treatment, improvement and / or prevention of an Alzheimer's disease. Experiments prove that the benzisoselenazolone quinoline derivative disclosed by the invention has excellent capacity of simulating glutathione peroxidase activity and restraining A beta aggregation and metal ion chelation, and is a multi-target Alzheimer's disease resistant active molecule. The benzisoselenazolone quinoline derivative is as shown in the formula I, wherein R1, R2, R3, R4 and X in the formula I are defined as the description.

The invention belongs to the field of traditional Chinese medicine preparation, relating to application of monomer gentiamarin extracted in the traditional Chinese medicine in preparing medicine for preventing and treating acute pancreatitis. In the invention, a model which is recognized by academic circles at home and abroad and is formed by continuously injecting caerulein to cause the acute pancreatitis of mice in enterocoelia and carrying out bezoar sodium taurocholate retrograde injection in cholangiopan so as to cause the acute pancreatitis of a rat, is adopted to study the action of the gentiamarin. The experiment result discloses that the gentiamarin has obvious function of resisting the acute pancreatitis caused by various reasons, and the action mechanism for resisting the acute pancreatitis is related to the releasing of the inflammatory factor, reduction of concentration of the tumor necrosis factor-alpha and interleukin-1beta in the serum or tissue, the increasing of the glutathione peroxidase activity in the serum or tissue, and the clearing of the radical and the expression of NF-kB P65.

Production of transparent acid containing tellurium with high glutathione oxide enzyme activity is artificial enzyme process with GPX activity. It consists of transparent acid activating, tellurium sodium hydride preparing, transparent acid tellurium reacting, tellurium transparent acid oxidizing and after processing. The production is carried out by activating transparent acid with tolylsulfonyl chlorin, preparing transparent acid benzene sulfonyl ester, dialyzing in distilled water, adding tellurium powder and sodium borohydride into anhydrous alcohol, heating and reverse flowing in nitrogen system, adding anaerobic glacial acetic acid, preparing alcohol solution of tellurium sodium hydride preparing, alcohol of tellurium sodium hydride reacting with transparent acid benzene sulfonyl ester solution, obtaining transparent acid containing the, the reacting system expose in air, centrifugal oxidizing, alcohol settling, and washing. It achieves simple process, high output, higher resistance to oxidation and GPX activity.

The invention belongs to the field of enzyme detection and discloses a method for extracting and determining the activity of glutathione peroxidase in tea trees. The method adopts leaves of the tea trees as an extracting material and comprises the steps of making a standard curve and extracting crude GSH-Px enzyme liquid, wherein 0.2 mol / L phosphate buffer liquid containing 1 mmol / L of EDTA-2Na and 4% of PPVP and having 6.2 of pH is used for extraction, and the measured activity of GSH-Px is largest. 10% TCA is easy to prepare and has good precipitation effect. The color development reaction reaches the maximum value at 6 min, and 5-thio-2-nitrobenzene anions have maximum light absorption value at 412 nm. The experiment method has the characteristics of easiness in material acquirement, simplicity for operation, easiness in control and reliable determination results.

The invention relates to the technical filed of food fermentation, and specifically relates to a preparation method of dark soy sauce with an anti-oxidation function. The dark soy sauce with the anti-oxidation function is prepared from the following raw materials: cordyceps militaris, soy sauce koji, oat, needle mushrooms, Chinese wolfberry fruits, hypsizygus marmoreus, bearded tooth mushrooms, a taste enhancer, crystal sugar, a sweetener, salt, and water. The preparation method of the dark soy sauce with the anti-oxidation function comprises the following steps of: preparing soup, preparing original soy sauce liquid, carrying out blending, carrying out concentrating, and carrying out sun-curing so as to obtain soy sauce liquid; and then, blending the sun-cured soy sauce liquid with the sweetener, the taste enhancer and the like, and carrying out cooking, filtering and filling so as to obtain the dark soy sauce. The dark soy sauce prepared by the preparation method is capable of improving total anti-oxidation capacity, superoxide dismutase activity and glutathione peroxidase activity of the serum and the liver, decreasing malondialdehyde content of the serum and the liver, reducing monoamine oxidase activity of the whole brain, improving peroxidase activity of the heart, and increasing immunoglobulin G content and immunoglobulin M content so as to achieve the functions of scavenging free radical, reduce heart oxidation and enhancing body immunity.

The invention discloses anti-oxidation young peptide egg functional feed, which is prepared from the following ingredients in parts by weight: 500 to 650 parts of corn, 200 to 300 parts of bean pulp, 15 to 23 parts of wheat bran, 8 to 12 parts of soybean oil, 0.3 to 0.4 part of selenium yeast, 0.08 to 0.12 part of vitamin E, 80 to 90 parts of mountain flour, 3 to 4 parts of table salt, 10 to 15 parts of calcium hydrophosphate and 18 to 22 parts of 2-percent laying hen compound premixes. The anti-oxidation young peptide egg functional feed has the advantages that the selenium yeast and the vitamin E are added into the feed, so that the contents of selenium and vitamin E in the eggs are improved; when people eat the eggs with high selenium and vitamin E content, the glutathione peroxidase activity in the human body can be improved; the anti-oxidation function is improved, so that the pigmentation and the black spot generation are reduced.

The invention discloses applications of total cannabinoid in preparing medicines for treating pancreatitis. The total cannabinoid is prepared by uniformly mixing 0.3-99.7 weigh parts of industrial hemp fructus cannabis extract and 99.7-0.3 weight part of industrial hemp cannabinoid. The clinical observation shows that total cannabinoid can reduce the activities of amylase and lipase in serum, and reduce the pancreas factor and the water content of the pancreatic tissue. The effect of resisting acute pancreatitis of total cannabinoid is realized by inhibiting the release of inflammatory factors, reducing the concentrations of tumor necrosis factors-alpha and the interleukin-1 beta in the serum or the tissue, and enhancing the activity of the glutathion peroxidase in the serum or the pancreatic tissue. Total cannabinoid has prevention and treatment effects for acute pancreatitis, and has the effects superior to that of cannabidiol. The invention further provides medicinal preparations of the total cannabinoid in treating the pancreatitis and the corresponding medicinal dosage forms of the medicinal preparations, the medicines with different dosage forms used clinically can be prepared with a conventional preparation method, and the use is convenient.

The invention provides a Chinese herbal medicine additive capable of improving the oxidation resistance of a parent body during the milking stage and an application method thereof. Astragalus root is dried, is crushed, and then is evenly added into the feedstuff for milking rats and sows and the extender of concentrated feedstuff for milking cows according to the amount of 1 percent. The medicine additive adopts a technical proposal that the rats are taken as model animals; the Chinese herbal medicine with a stable antioxidation effect is selected from a plurality of milk promoting Chinese herbal medicines; and then tests on the rats, sows and cows are carried out. Finally, 1 percent of astragalus root added into the feedstuff for milking rats and sows and the extender of concentrated feedstuff for milking cows is found to have the best antioxidation effect. The Chinese herbal medicine additive improves the activity of superoxide dismutase and glutathione peroxidase in the blood serum and cardiac muscle and the general oxidation resistance level for the rats, sows and cows in the medium and later milking stage, and remarkably reduces the level of malonaldehyde in the blood serum, liver, kidney and cardiac muscle in the later milking stage (P<0.05 or P<0.01). The Chinese herbal medicine additive can improve the health condition of mammals after application, thereby bringing about good economical and ecological benefits.

The invention provides tangut barberry bark fruit polysaccharide which is an extract from a Berberis dasystachya Maxim. fruit; the polysaccharide content of the extract is 60-95% w / w. Researches show that tangut barberry bark fruit polysaccharide has a favorable blood glucose reducing function, can increase the insulin amount, and has a certain adjusting effect on the activities of corresponding ROS metabolism related enzymes such as superoxide dismutase, catalase and glutathione peroxidase. Besides, the effect of the high dose group of a tangut barberry bark fruit is equivalent to that of glibenclamide, while though glibenclamide is a chemical drug for treating diabetes, taking of a large amount of glibenclamide for a long time can cause hypoglycemia and nephropathy finally, or even death; otherwise, the tangut barberry bark fruit is an edible berry, is favorable in safety, and is more suitable for being used as a food, a health product or a drug for treating and preventing diabetes.

The invention discloses a preparation method of low-molecular weight oxidized konjac glucomannan, fish feed prepared from the low-molecular weight oxidized konjac glucomannan, and a feeding method of the fish feed. After schizothorax prenanti is fed with the fish feed, the serum IgM activity is enhanced, the contents of ACH50 and lysozyme are obviously increased, the superoxide dismutase activity and glutathione peroxidase activity are improved, the content of malonaldehyde is reduced, and the contents of relative total bacteria of aeromonas hydrophila and bacillus in the foregut, midgut and hindgut of the schizothorax prenanti are obviously increased. After the schizothorax prenanti ingests the feed, the immunity is obviously enhanced, and the survival rate is obviously increased, thereby improving the economic benefits and reducing the breeding cost.

A process for preparing selenopapain from papain and sodium selenohydride through dissolving papain in phosphate solution, adding excessive sodium selenohydride, seleno substitution under protection of N2, freeze drying, dissolving in phosphate solution centrifugal removal of Se, removing salt, collecting protein peaks, and freeze drying. Its advantages are simple process high activity of glutathion peroxidase, and excellent antioxidizing power.

The invention discloses a lactobacillus fermentum (Lactobacillus fermentum) JX306 with anti-oxidation function and its application, belonging to the technical field of bioengineering. The bacteria has strong DPPH free radical and hydroxyl free radical scavenging ability, high reducing power, strong Fe 2+ Chelating ability and good anti-lipid peroxidation ability, and has good bile salt resistance ability, gastrointestinal fluid resistance ability and hydrogen peroxide tolerance; it can also significantly increase glutathione peroxidase activity, reduce Malondialdehyde levels can significantly up-regulate the levels of five antioxidant enzymes including TR3, Prdx1, Gsr, Gpx1, and nuclear transcription factor Nrf2, and can significantly alleviate the histopathological damage induced by D-gal. The Lactobacillus fermentum JX306 of the invention has a great effect on improving the antioxidant performance of food as a fermented strain, and has wide application prospects.

Production of transparent acid containing tellurium with high glutathione oxide enzyme activity is artificial enzyme process with GPX activity. It consists of transparent acid activating, tellurium sodium hydride preparing, transparent acid tellurium reacting, tellurium transparent acid oxidizing and after processing. The production is carried out by activating transparent acid with tolylsulfonyl chlorin, preparing transparent acid benzene sulfonyl ester, dialyzing in distilled water, adding tellurium powder and sodium borohydride into anhydrous alcohol, heating and reverse flowing in nitrogen system, adding anaerobic glacial acetic acid, preparing alcohol solution of tellurium sodium hydride preparing, alcohol of tellurium sodium hydride reacting with transparent acid benzene sulfonyl ester solution, obtaining transparent acid containing the, the reacting system expose in air, centrifugal oxidizing, alcohol settling, and washing. It achieves simple process, high output, higher resistance to oxidation and GPX activity.

The invention discloses a blueberry anthocyanidin product capable of reducing the increment of free radicals in the human body and a preparation method thereof. Multiple natural plant nutrients are compounded, and a concentrated tablet is prepared by a multidimensional moulding technology. The product and preparation method have following advantages: (1) most raw materials are wild plants and do not have any side effect; (2) wild blueberry anthocyanidin freeze-dried powder and dahurian rose fruit extract have a good effect on eliminating free radicals in human body; (3) the product is rich inplant active selenium, can enhance the activity of glutathione peroxidase in human body, and reduces the number of free radicals in human body; (4) the product can obviously eliminate free radicals inthe human body and is obviously beneficial for the organs and tissues of organisms; and (5) the product can be automatically absorbed and converted in human body, the ratio of active substances is reasonable and scientific, and the absorption rate is high.

The invention provides an anti-intestinal aging composition and an application thereof, and relates to the field of functional foods. The anti-intestinal aging composition comprises the following rawmaterials in percentages by mass: 5-10% of ellagic acid, 0.001-0.01% of vitamin B6, 5%-10% of a fruit and vegetable fermented material, 10-20% of sorbitol, 20-40% of oligosaccharide, 5-20% of water-soluble dietary fibers, and 0-20% of an auxiliary material. Through the combination of the above raw materials, the composition can effectively reduce intestinal harmful bacteria (LPS generation bacteria), adjust composition of intestinal flora and promote diversity of the intestinal flora, further improve host glutathione peroxidase activity and superoxide dismutase activity, reduce the content ofmalondialdehyde, and achieve the purposes of enhancing intestinal antioxidant activity and delaying intestinal aging.

The invention discloses a quantitative determination method for seminal plasma glutathione peroxidase activity and a kit, and the method comprises the following steps: taking a seminal fluid specimen, centrifuging, taking supernate into a centrifuge tube to obtain a seminal plasma specimen, adding a reagent 1 and a reagent 2 into the seminal plasma specimen, uniformly mixing, and determining an absorbance value A (determination 1), wherein the reagent 1 is a phosphate buffer solution containing reduced glutathione, EDTA-Na2 and sodium azide, and the reagent 2 is a phosphate buffer solution containing glutathione reductase and NADPH; adding a reagent 3 into the seminal plasma specimen, carrying out uniform mixing and reaction, and measuring the absorbance value A and the absorbance value 2, wherein the reagent 3 is a phosphate buffer solution containing cumene hydroperoxide; obtaining a blank control group in the same step, measuring an absorbance value A blank 1, adding a reagent 3, uniformly mixing, reacting, and measuring an absorbance value A blank 2; and calculating the measured value according to the following formula: GSH-P* activity (n mol / min / mL) = [(deltaA measurement-deltaA blank) / epsilon / d * V total * 106] / V sample / T.

The invention discloses a method for researching influence of exogenous selenium on selenium content, glutathione peroxidase activity, and quality of tea. The method comprises the following steps of:(1) setting a concentration gradient of 6 amino acid chelated selenium liquid fertilizer in a test, choosing cloudy day for leaching after the fertilizer is mixed with water, and setting 3 repetitions, wherein the area of each plot is consistent; (2) plucking leaves with two leaves and one core after applying selenium, rinsing 3 times with distilled water, drying with filter paper, taking fresh leaves to measure antioxidant enzymes, performing the deactivation of enzymes on the remaining leaves, and then drying, and smashing and sifting to obtain tea pulverized materials; and (3) using the teapulverized materials obtained in the step 2 to determine the selenium content, glutathione peroxidase activity, tea polyphenol content, and tea polysaccharide content of the tea. The invention makesa preliminary discussion on the influence of exogenous selenium on the selenium content, glutathione peroxidase activity, and quality of tea, and provides a theoretical basis so as to further study the dynamic changes of selenium in tea and the regulation of selenium on physiological mechanisms of tea.

The invention provides an application of rosa roxburghii tratt fruit polysaccharides in preparation for blood glucose-reducing medicines, heath products or foods. Researches on the application provided by the invention find that the rosa roxburghii tratt fruit polysaccharides have a good blood glucose-reducing function, can increase an insulin amount, and have a regulation effect on the activities of the corresponding ROS metabolism-related enzymes, that is, the activities of superoxide dismutase, catalase, and glutathion peroxidase to a certain extent. Meanwhile, the application provided by the invention further finds that the effect of a rosa roxburghii tratt fruit high-dose group is equal to the effect of glibenclamide, although glibenclamide is a first-line medicine for treating diabetes, glibenclamide finally causes the hypoglycaemia and kidney diseases of patients, and even causes death in case of being greatly taken for a long time; conversely, rosa roxburghii tratt fruits are edible berries, good in safety, and more suitable for being used as foods, health products or medicines for treating and preventing diabetes.

The invention provides tangut barberry bark fruit polysaccharide which is an extract from a Berberis dasystachya Maxim. fruit; the polysaccharide content of the extract is 60-95% w / w. Researches show that tangut barberry bark fruit polysaccharide has a favorable blood glucose reducing function, can increase the insulin amount, and has a certain adjusting effect on the activities of corresponding ROS metabolism related enzymes such as superoxide dismutase, catalase and glutathione peroxidase. Besides, the effect of the high dose group of a tangut barberry bark fruit is equivalent to that of glibenclamide, while though glibenclamide is a chemical drug for treating diabetes, taking of a large amount of glibenclamide for a long time can cause hypoglycemia and nephropathy finally, or even death; otherwise, the tangut barberry bark fruit is an edible berry, is favorable in safety, and is more suitable for being used as a food, a health product or a drug for treating and preventing diabetes.

The invention provides an application of rosa roxburghii tratt fruit polysaccharides in preparation for blood glucose-reducing medicines, heath products or foods. Researches on the application provided by the invention find that the rosa roxburghii tratt fruit polysaccharides have a good blood glucose-reducing function, can increase an insulin amount, and have a regulation effect on the activities of the corresponding ROS metabolism-related enzymes, that is, the activities of superoxide dismutase, catalase, and glutathion peroxidase to a certain extent. Meanwhile, the application provided by the invention further finds that the effect of a rosa roxburghii tratt fruit high-dose group is equal to the effect of glibenclamide, although glibenclamide is a first-line medicine for treating diabetes, glibenclamide finally causes the hypoglycaemia and kidney diseases of patients, and even causes death in case of being greatly taken for a long time; conversely, rosa roxburghii tratt fruits are edible berries, good in safety, and more suitable for being used as foods, health products or medicines for treating and preventing diabetes.

The invention discloses a drug for treating pancreatitis. The drug is prepared by evenly mixing 0.3-99.7 parts by weight of industrial hemp seed extract and 99.7-0.3 parts by weight of industrial cannabinoid. It is found through clinical observation that the drug can decrease the activity of serum amylase and lipase and decrease the pancreas coefficient and the water content of pancreatic tissue. The drug can resist acute pancreatitis by inhibiting release of inflammatory factors, reduce concentrations of tumor necrosis factor-alpha and interleukin-1beta in serum or tissue and enhance the activity of glutathion peroxidase in the serum or the pancreatic tissue, and the effect is superior to that of cannabidiol. The drug can be prepared into various different clinically-used dosage forms through a conventional preparation method and is convenient to use.

A process for preparing selenopapain from papain and sodium selenohydride through dissolving papain in phosphate solution, adding excessive sodium selenohydride, seleno substitution under protection of N2, freeze drying, dissolving in phosphate solution centrifugal removal of Se, removing salt, collecting protein peaks, and freeze drying. Its advantages are simple process high activity of glutathion peroxidase, and excellent antioxidizing power.