Lactobacillus fermentum JX306 with antioxidant function, and application thereof

A Lactobacillus fermentum, antioxidant technology, applied in the field of bioengineering to achieve the effect of inhibiting tissue pathological damage

Active Publication Date: 2020-01-14
SHANDONG AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, there is no patent or patent application for Lactobacillus fermentum, which is isolated from traditional Chinese fermented vegetables a

Method used

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  • Lactobacillus fermentum JX306 with antioxidant function, and application thereof
  • Lactobacillus fermentum JX306 with antioxidant function, and application thereof
  • Lactobacillus fermentum JX306 with antioxidant function, and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1: Obtaining and identification of Lactobacillus fermentum JX306

[0031] 1. Separation and purification of Lactobacillus fermentum JX306

[0032] The solution of Laotan sauerkraut in Ji'an, Jiangxi was diluted with sterile saline (0.85%, w / v) successively, and spread on the MRS agar plate (containing 0.75% CaCO2 in the MRS liquid medium). 3 and 1.5% agar powder). After static culture at 37°C for 48 hours, the colonies were separated according to their size, color, shape and edge characteristics, and repeatedly streaked and purified on the MRS solid medium until pure, single colonies appeared on the solid medium. The isolated lactic acid bacteria were continuously passaged for more than 5 times, and the strains with stable growth and fast growth were screened out, cultured at 37°C until the stable phase, and all strains were stored at -80°C in MRS liquid medium containing 20% ​​glycerol .

[0033] 2. Identification of Lactobacillus fermentum JX306

[0034] 1...

Embodiment 2

[0061] Example 2. Determination of Lactobacillus fermentum JX306 Antioxidant Index in Vitro

[0062] Lactobacillus fermentum JX306 was inoculated with 1% (v / v) in 1.0mL MRS liquid medium, cultured at 37°C for 24h, centrifuged at 8000×g for 10min, washed with sterile water twice to adjust the bacterial cells The concentration is 4×10 8 CFU / mL.

[0063] 1. DPPH free radical scavenging experiment

[0064] Add 1.0mL DPPH solution with a concentration of 0.2mmol / L to 1.0mL bacterial cells (dissolve DPPH in absolute ethanol), mix well, react in the dark at room temperature for 30min, centrifuge at 8000×g for 10min, and take the supernatant at a wavelength of 517nm Measure the absorbance. The measurement results showed that the DPPH free radical scavenging rate of Lactobacillus fermentum JX306 was 37.29%, showing better free radical scavenging ability.

[0065] 2. Hydroxyl radical scavenging ability test

[0066] 1.0mL bacterial cells, 0.5mL o-phenanthroline (2.5mmol / L), 1.0mL P...

Embodiment 3

[0073] Example 3. In vitro tolerance determination of Lactobacillus fermentum JX306

[0074] Lactobacillus fermentum JX306 was inoculated with 1% (v / v) in 1.0mL MRS liquid medium, cultured at 37°C for 24h, centrifuged at 8000×g for 10min, washed with sterile water twice to adjust the bacterial cells The concentration is 4×10 8 CFU / mL.

[0075] 1. Determination of bile salt tolerance

[0076] Adjust the bacterial cell concentration to 4×10 8 CFU / mL, resuspended in 1 mL of sterile saline solution containing 1 mg / mL trypsin and 0.5% (w / v) bile salts. Cells were incubated at 37°C for 1h, 2h, and 3h, diluted and spread on MRS plates. After incubation at 37°C for 48h, the survival rate of the strains was determined by plate counting. The measurement results are shown in Table 5, and the results showed that the survival rate of the strains showed a downward trend as time went on.

[0077] The tolerance to bile salts of the bacterial strains in table 5

[0078]

[0079] 2. Re...

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Abstract

The invention discloses lactobacillus fermentum JX306 with an antioxidant function and application thereof, and belongs to the technical field of bioengineering. The lactobacillus fermentum JX306 hasa high DPPH free radical and hydroxyl radical scavenging ability, high reducing power, a high Fe2+ chelating ability, a favorable anti-lipid peroxidation ability, and favorable bile salt tolerance, gastrointestinal fluid resistivity and hydrogen peroxide tolerance; and the glutathione peroxidase activity can be remarkably improved, the malondialdehyde level is reduced, the levels of five antioxidant enzymes such as TR3, Prdx1, Gsr, Gpx1 and a nuclear transcription factor Nrf2 are remarkably improved, and the D-gal induced histopathological lesion can be remarkably relieved. The lactobacillus fermentum JX306 provided by the invention plays a big role on improving the oxidation resistance of food with the lactobacillus fermentum JX306 as a fermenting microbe, and has a wide application prospect.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, and in particular relates to Lactobacillus fermentum JX306 with anti-oxidation function and its application. Background technique [0002] Oxidative stress is considered to be an imbalance between high levels of reactive oxygen species and low activity of antioxidant mechanisms, causing various diseases such as amyotrophic lateral sclerosis, asthma, allergies, diabetes, and further accelerating aging. Antioxidants butylhydroxyanisole (BHA), butylated hydroxytoluene (BHT) and tert-butylhydroquinone (TBHQ) in food have been used to control oxidative stress, but their safety has been questioned in recent years. Lactic acid bacteria, on the other hand, are important probiotics in the human gut. When consumed as dietary supplements or through foods (whether fermented or fortified), they control oxidative stress in the body or tissues and are beneficial to health. [0003] Lactic acid bacteria ...

Claims

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Application Information

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IPC IPC(8): C12N1/20A23C9/123A23L33/135C12R1/225
CPCA23C9/123A23L33/135C12R2001/225C12N1/205A23V2400/143
Inventor 杨正友张迪赵凤春李创
Owner SHANDONG AGRICULTURAL UNIVERSITY
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