Tangut barberry bark fruit polysaccharide, as well as preparation method and use thereof

A technology of fruit and polysaccharides, applied in the field of polysaccharides of japonica fruit and its preparation, can solve problems such as no research reports on polysaccharides of japonica japonica fruit, achieve good blood sugar-lowering function, increase enzyme activity, and improve lipid metabolism disorders

Active Publication Date: 2015-09-30
DELINGHA LINSHENG BIOTECH DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] There is no research report on the ...

Method used

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  • Tangut barberry bark fruit polysaccharide, as well as preparation method and use thereof
  • Tangut barberry bark fruit polysaccharide, as well as preparation method and use thereof
  • Tangut barberry bark fruit polysaccharide, as well as preparation method and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Embodiment 1: the preparation of the polysaccharide of the fruit of japonica

[0045] 1) Degreasing of the fruit of C. japonica: Take 400g of ripe fruit of C. chinensis (i.e., Berberis straight panicle) and dry it, crush it and sieve it; mix the fruit of C. japonica with petroleum ether, extract it by ultrasonic after mixing, and centrifuge to remove the extract and residue Filter, evaporate the petroleum ether to obtain the degreased thorn fruit residue;

[0046] 2) Removal of monosaccharides from the fruit of Citrus japonica: mix the residue of the fruit of Citrus japonica with ethanol, the concentration of ethanol is 70-85% v / v for reflux extraction, centrifuge to remove the extract, filter the residue and evaporate the ethanol to get rid of the ethanol. Monosaccharide yellow thorn fruit residue;

[0047] 3) Extraction of the crude polysaccharide of C. japonica: mixing the residue of the fruit of C. japonica from which monosaccharides have been removed obtained in s...

Embodiment 2

[0050] Embodiment 2: the preparation of the polysaccharide of the fruit of japonica

[0051] 1) Degreasing of the fruit of C. japonica: take 400 g of ripe fruit of C. chinensis (i.e. Berberis straight panicle) and dry it, crush it and sieve it; mix the fruit of C. japonica with petroleum ether, extract by refluxing, centrifuge to remove the extract, filter the residue, Evaporate the sherwood oil to obtain the degreasing fruit residue;

[0052] 2) Removal of simple sugars from the fruit of Citrus japonica: mix the residue of the fruit of Citrus japonica with ethanol, the ethanol concentration is 70-85% v / v, ultrasonically extract, centrifuge to remove the extract, filter the residue and evaporate the ethanol to obtain Residue of removing monosugar thorn fruit;

[0053] 3) Extraction of the crude polysaccharide of C. japonica: mixing the residue of the fruit of C. japonica from which monosaccharides have been removed obtained in step 2) with pure water, extracting by warm soaki...

Embodiment 3

[0056] Embodiment 3: the preparation of the polysaccharide of the fruit of japonica

[0057] 1) Degreasing of the fruit of C. japonica: take 400 g of mature fruit of C. chinensis (i.e. Berberis straight panicle) and dry it, crush it and sieve it; mix the fruit of C. japonica with petroleum ether, reflow and extract after ultrasonication, and remove the extract by centrifugation. The residue is filtered, and the petroleum ether is evaporated to obtain the residue of the degreased thorn fruit;

[0058] 2) Removal of simple sugars from the fruit of Citrus japonica: mix the residue of the fruit of Citrus japonica with ethanol, the ethanol concentration is 70-85% v / v, ultrasonically extract, centrifuge to remove the extract, filter the residue and evaporate the ethanol to obtain Residue of removing monosugar thorn fruit;

[0059] 3) Extraction of the crude polysaccharide of C. japonica: mixing the residue of the fruit of C. japonica from which monosaccharides have been removed obt...

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Abstract

The invention provides tangut barberry bark fruit polysaccharide which is an extract from a Berberis dasystachya Maxim. fruit; the polysaccharide content of the extract is 60-95% w/w. Researches show that tangut barberry bark fruit polysaccharide has a favorable blood glucose reducing function, can increase the insulin amount, and has a certain adjusting effect on the activities of corresponding ROS metabolism related enzymes such as superoxide dismutase, catalase and glutathione peroxidase. Besides, the effect of the high dose group of a tangut barberry bark fruit is equivalent to that of glibenclamide, while though glibenclamide is a chemical drug for treating diabetes, taking of a large amount of glibenclamide for a long time can cause hypoglycemia and nephropathy finally, or even death; otherwise, the tangut barberry bark fruit is an edible berry, is favorable in safety, and is more suitable for being used as a food, a health product or a drug for treating and preventing diabetes.

Description

technical field [0001] The invention belongs to the field of health care products, and in particular relates to polysaccharides from japonica japonica fruit, a preparation method and application thereof. technical background [0002] Yellow thorn, Berberis dasystachya Maxim., a plant of Berberidaceae, is a traditional wild berry that is used as medicine and food by Mongolian, Tibetan, Uygur and other ethnic minorities like white thorn and seabuckthorn (black thorn), collectively referred to as "Qinghai three prick". At present, for Nitraria and black thorn, the research and application are very thorough, but for the yellow thorn, its component research and functional application are still relatively blank. [0003] "Qinghai Commonly Used Chinese Herbal Medicine Handbook" has detailed records on the bark of the yellow bark (ie root bark): the bark of the bark of the dicotyledonous plant Berberidaceae Berberis straight panicle. The medicinal function of its fruit has also be...

Claims

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Application Information

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IPC IPC(8): C08B37/00A61K36/29A23L1/29A61P39/06A61P35/00A61P3/10A61P3/00A61K31/715
Inventor 孟昭军韩丽娟索有瑞
Owner DELINGHA LINSHENG BIOTECH DEV
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