Method for dyeing silk by using laccase-catalyzed tea polyphenols

A technology of laccase catalyzing tea and tea polyphenols, which is applied in the field of textile dyeing, can solve the problems of poor water solubility, adverse effect on silk fabric dyeing effect, and affecting enzyme activity, etc., and achieve mild dyeing conditions, good dyeing effect, and good antibacterial effect

Inactive Publication Date: 2012-07-11
SUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, when dyeing, some phenolic compounds and amine compounds have to be dissolved with the help of organic solvents due to their poor water solubility.
Since most organic solvents will affect the activity of enzymes, the dyeing effect of silk fabrics dyed by this dyeing system will also be adversely affected

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] (1) Determination of laccase activity: The laccase activity was determined by oxidizing syringaldehyde nitrous under aerobic conditions, and the color of the generated syringaldehyde hydrazine oxidation product was measured at 530 nm by UV-visible spectrophotometry (violet blue). The analysis conditions are: under the conditions of 30°C and acetate buffer (pH 6.5), when using syringaldazine as the substrate, the amount of enzyme required for 3mL of the reaction substrate to change the absorbance by 0.001 per minute at the wavelength of 530nm . The unit of enzyme activity is U.

[0025] (2) Laccase catalyzed the dyeing of tea polyphenols on silk: tea polyphenols were 3 g / L, pH was adjusted to 3.5, 4.0, 4.5, 5.0, 5.5, 6.0 and 6.5 with acetate buffer, and the dyeing bath ratio was 20:1, add silk crepe chine, add laccase obtained from Rhus vernicifera at a concentration of 2.5 U / mL (purchased from SIGMA company), heat up at 50°C at a rate of 2°C / min, and keep warm for 2 h...

Embodiment 2

[0028] Tea polyphenols were 3 g / L, the pH was adjusted to 5.0 with acetate buffer, the dyeing bath ratio was 20:1, silk crepe chine was added, and laccase obtained from Rhus vernicifera at a concentration of 2.5 U / mL (purchased from SIGMA Company), heating at 40, 50, 60, 70 and 80°C at a rate of 2°C / min, and keeping it warm for 2 hours, to obtain dark yellow dyed silk fabrics.

[0029] Table 1 provides the color parameters of silk crepe chine dyed fabrics catalyzed by tea polyphenols at different temperatures in this example.

[0030] Table 1

[0031] temperature (℃) 40 50 60 70 80 L* 81.64 77.61 79.66 78.88 75.85 a* 5.11 6.39 5.90 6.19 6.89 b* 18.90 21.48 18.67 18.43 19.18

Embodiment 3

[0033]Tea polyphenols were 3 g / L, the pH was adjusted to 5.0 with acetate buffer, the dyeing bath ratio was 20:1, and silk crepe chine was added at concentrations of 1, 2, 3, 4, 5 and 6 U / mL from The laccase obtained from Rhus vernicifera (purchased from SIGMA Company) was heated to 50°C at a rate of 2°C / min and kept at a temperature of 2 hours to obtain dark yellow dyed silk fabrics of different shades.

[0034] Table 2 provides the color parameters of the enzyme-catalyzed tea polyphenol dyeing of silk crepe chine fabric under different laccase dosages in this example.

[0035] Table 2

[0036] Laccase concentration (U / mL) 1 2 3 4 5 6 L* 81.01 80.22 79.01 77.59 77.77 77.60 a* 5.26 5.70 6.13 6.73 6.68 6.71 b* 15.46 17.34 19.75 22.99 22.14 22.65

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Abstract

The invention discloses a method for dyeing silk by using laccase-catalyzed tea polyphenols, and belongs to the field of textile dyeing. Tea polyphenols, laccase and a pH buffer are taken as a dye solution. The method comprises the following steps of: dissolving the tea polyphenols in water, regulating the pH of a dye bath by using an acetate buffer until the dye bath is weakly acidic, adding the laccase, performing polymerization reaction on the tea polyphenols under the catalytic action of the laccase, and dyeing a silk fabric. The method for dyeing the silk by using the laccase-catalyzed tea polyphenols is simple and practicable, is mild in dyeing condition and has the advantages of environment friendliness and low probability of scratch defects.

Description

technical field [0001] The invention relates to the dyeing application of oxidoreductase on silk, in particular to a dyeing method in which laccase is used as a catalyst for the polymerization of tea polyphenols to simultaneously catalyze the polymerization of tea polyphenols and dye silk, which belongs to textile dyeing field. Background technique [0002] In the traditional dyeing process, silk is mainly dyed with acid dyes, neutral dyes and reactive dyes. The traditional process inevitably has the disadvantages of high dyeing temperature, high energy consumption, large water consumption and large amount of chemicals. What's more, if the technical parameters in the rope dyeing process are not properly controlled, the fabric will be scratched due to the high dyeing temperature and the high friction between the fabrics, and "gray damage" defects will appear. If low-temperature dyeing is used, the occurrence of "gray damage" defects can be effectively avoided, and energy con...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): D06P1/00D06P3/04C12S11/00
Inventor 唐人成孙莎莎
Owner SUZHOU UNIV
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