Preparation method of low-molecular weight oxidized konjac glucomannan, fish feed prepared from low-molecular weight oxidized konjac glucomannan, and feeding method of fish feed
A mannan and low molecular weight technology, applied in the field of biological feed, can solve the problems of resource reduction and improper feeding method, and achieve the effects of improving survival rate, improving immune effect and reducing drug dosage.
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[0034] Preparation of low molecular weight oxidized konjac glucomannan: 1. Weigh 100g of konjac flour and dissolve it in a pre-prepared 25ml 50% ethanol solution and stir evenly to form mixture A; 2. Seal the container containing mixture A with plastic wrap , And then use a magnetic stirrer to stir the mixture A for 35 minutes, add 30% H2O 27ml, 7ml, 3.5ml at the 10th, 20th, and 30th minutes of the stirring process, and uncover the fresh-keeping film when adding 30% H2O2 , And then cover after adding, add a total of 17.5ml 30% H2O2 to obtain Mixture B; 3. Put Mixture B together with the container into a constant temperature water bath, the temperature is 40℃, and the water level is just below the height of Mixture B. The height of the container, the container is sealed with cling film, the water bath time is 4h, and the mixture C is obtained; 4. After the water bath, the mixture C is filtered with a filter device, and then the white solid powder in the Buchner funnel The substa...
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