Preparation method of low-molecular weight oxidized konjac glucomannan, fish feed prepared from low-molecular weight oxidized konjac glucomannan, and feeding method of fish feed

A mannan and low molecular weight technology, applied in the field of biological feed, can solve the problems of resource reduction and improper feeding method, and achieve the effects of improving survival rate, improving immune effect and reducing drug dosage.

Inactive Publication Date: 2017-12-12
SICHUAN AGRI UNIV
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

[0005] Another reason for the drastic reduction of Schizothorax resources is the improper feeding method. Therefore, there is an urgent need for a standardized feeding method to improve the breeding efficiency of Schizothorax

Method used

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  • Preparation method of low-molecular weight oxidized konjac glucomannan, fish feed prepared from low-molecular weight oxidized konjac glucomannan, and feeding method of fish feed
  • Preparation method of low-molecular weight oxidized konjac glucomannan, fish feed prepared from low-molecular weight oxidized konjac glucomannan, and feeding method of fish feed
  • Preparation method of low-molecular weight oxidized konjac glucomannan, fish feed prepared from low-molecular weight oxidized konjac glucomannan, and feeding method of fish feed

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Embodiment

[0034] Preparation of low molecular weight oxidized konjac glucomannan: 1. Weigh 100g of konjac flour and dissolve it in a pre-prepared 25ml 50% ethanol solution and stir evenly to form mixture A; 2. Seal the container containing mixture A with plastic wrap , And then use a magnetic stirrer to stir the mixture A for 35 minutes, add 30% H2O 27ml, 7ml, 3.5ml at the 10th, 20th, and 30th minutes of the stirring process, and uncover the fresh-keeping film when adding 30% H2O2 , And then cover after adding, add a total of 17.5ml 30% H2O2 to obtain Mixture B; 3. Put Mixture B together with the container into a constant temperature water bath, the temperature is 40℃, and the water level is just below the height of Mixture B. The height of the container, the container is sealed with cling film, the water bath time is 4h, and the mixture C is obtained; 4. After the water bath, the mixture C is filtered with a filter device, and then the white solid powder in the Buchner funnel The substa...

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Abstract

The invention discloses a preparation method of low-molecular weight oxidized konjac glucomannan, fish feed prepared from the low-molecular weight oxidized konjac glucomannan, and a feeding method of the fish feed. After schizothorax prenanti is fed with the fish feed, the serum IgM activity is enhanced, the contents of ACH50 and lysozyme are obviously increased, the superoxide dismutase activity and glutathione peroxidase activity are improved, the content of malonaldehyde is reduced, and the contents of relative total bacteria of aeromonas hydrophila and bacillus in the foregut, midgut and hindgut of the schizothorax prenanti are obviously increased. After the schizothorax prenanti ingests the feed, the immunity is obviously enhanced, and the survival rate is obviously increased, thereby improving the economic benefits and reducing the breeding cost.

Description

Technical field [0001] The invention relates to the technical field of biological feeds, in particular to a preparation method of low molecular weight oxidized konjac glucomannan, and a fish feed prepared therefrom and a feeding method. Background technique [0002] Schilaphus has a low immunity and is more sensitive to external stimuli. In recent years, due to environmental pollution and other reasons, the amount of fish resources has been drastically reduced, resulting in economic damage to fishermen and a sharp increase in fish meat market prices. Therefore, there is an urgent need to develop a feed that can improve the immune performance of the slit stomach class. [0003] Konjac glucomannan, also known as konjac polysaccharide, is composed of D-mannose and D-glucose in a ratio of 1.6:1 connected by β-1,4 glycosidic bonds. It is a known natural polymer heteropolysaccharide with the highest viscosity. Konjac glucomannan is an immunomodulator, which has the effect of improving i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K50/80A23K20/163A01K61/10C08B37/02C07H3/06C07H1/08
CPCA23K20/163A23K50/80A01K61/10C07H1/08C07H3/06C08B37/009Y02A40/81Y02A40/818
Inventor 邬应龙林德荣严秋萍周伟龙小梅阳元梦陈明睿赵嘉琪冯莉梅吕珍珍何梅段正星王冰莹吴贺君周敏陈赛艳张清吴定涛刘耀文秦文陈洪陈姝娟李素清
Owner SICHUAN AGRI UNIV
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