A preparation method of an antioxidant polypeptide of Chlorella vulgaris

A technology of antioxidant peptides and chlorella, applied in the biological field, to achieve good in vitro scavenging of DPPH and hydroxyl radicals, good antioxidant activity, and improved extraction rates

Active Publication Date: 2019-01-15
FUJIAN NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Alzahrani et al. extracted three kinds of microalgae proteins by ultrasonic method in 2017, and then separately enzymolyzed them with alkaline protease, flavor protease and trypsin, an

Method used

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  • A preparation method of an antioxidant polypeptide of Chlorella vulgaris
  • A preparation method of an antioxidant polypeptide of Chlorella vulgaris
  • A preparation method of an antioxidant polypeptide of Chlorella vulgaris

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Embodiment 1

[0030] 1. Preparation of Chlorella cell wall enzymolysis solution and specific proteolysis solution

[0031] The cellulase, pectinase and various proteases used were purchased from Beijing Soleibao Technology Co., Ltd.

[0032] 1) The composition ratio of the chlorella cell wall enzymatic hydrolysis solution is:

[0033]

[0034] After the above-mentioned chlorella cell wall enzymatic hydrolysis solution is prepared, the pH is adjusted to 4.0 with 36% concentrated hydrochloric acid.

[0035] 2) The proportion of the specific proteolytic solution components is:

[0036]

[0037]

[0038] After preparing the above-mentioned specific proteolysis solution, adjust the pH to 9.5 with 1mol / L NaOH solution;

[0039] 2. Chlorella antioxidant polypeptide is prepared by the following steps:

[0040] 1) Weigh 500g of chlorella powder and add it to 4.5L 0.3mol / L NaOH dilute alkali solution, soak for 60min at 60°C to obtain pretreated chlorella liquid;

[0041] 2) The pretreated chlorella liquid obtaine...

Embodiment 2

[0064] 1. Preparation of Chlorella cell wall enzymolysis solution and specific proteolysis solution

[0065] The cellulase, pectinase and various proteases used were purchased from Beijing Soleibao Technology Co., Ltd.

[0066] 1) The weight ratio of the chlorella cell wall enzymatic hydrolysate is:

[0067]

[0068]

[0069] Adjust the pH to 4.0 with 36% concentrated hydrochloric acid;

[0070] 2) The weight ratio of the specific proteolysis solution is:

[0071]

[0072] Adjust the pH to 7.5~9.5 with 1mol / L NaOH solution;

[0073] 2. Prepared by the following steps:

[0074] 1) Weigh 750g of chlorella powder and add it to 4.25L 0.3mol / L NaOH dilute alkali solution, soak for 60min at 60°C to obtain pretreated chlorella liquid;

[0075] 2) The pretreated chlorella liquid obtained in step 1) was homogenized twice at a pressure of 55 MPa and centrifuged at 5000 rpm for 20 min. The supernatant A obtained was temporarily stored at 4°C, and the precipitate obtained Add to the chlorella cell wa...

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Abstract

The invention provides a preparation method of chlorella antioxidant polypeptide, which is characterized in that the chlorella protein is extracted by high-pressure homogenization method combined withcellulase/pectinase enzymatic hydrolysis, enzymatic hydrolysis of a specific protease hydrolysate and ultrafiltration membrane separation to obtain chlorella enzymatic hydrolytic peptide with different molecular weight intervals. The molecular weight range of the present invention after enzymatic hydrolysis by a specific protease hydrolysate is 0-3.5 KDa chlorella polypeptides have better scavenging activity of DPPH and hydroxyl radical in vitro than other protease hydrolysates and other chlorella polypeptides with other molecular weight intervals. The ability of scavenging ROS clusters of hepatocellular carcinoma cells in vitro, the inhibition of MDA formation in vivo and the promotion of GSH-Px activity in vivo are more effective than those in vivo. A Chlorella active peptide with bet antioxidant function is prepared from enzyme hydrolysate of specific protease, which is beneficial to that development and utilization of health food and pharmaceutical product with antioxidant function.

Description

Technical field [0001] The invention provides a preparation method of chlorella antioxidant polypeptide, which belongs to the field of biotechnology. Background technique [0002] Chlorella is a pervasive single-celled green algae. It is widely distributed in nature. It can grow and reproduce with organic carbon sources under heterotrophic conditions. It has a large biomass and is the only one on earth that can grow A creature that grows 4 times an hour. Chlorella is extremely nutritious. The crude protein content is as high as 50%, and the essential amino acid composition is basically balanced. It also contains unsaturated fatty acids, bioactive polysaccharides, nucleic acids, vitamins, trace elements, minerals, chlorophyll, etc., with extremely high nutritional value It has a variety of biological pharmacological activities such as preventing and treating peptic ulcer, anti-tumor, enhancing immunity, anti-radiation, anti-pathogenic microorganism, preventing and treating anemia...

Claims

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Application Information

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IPC IPC(8): C12P21/06C07K1/34
CPCC12P21/06C07K1/34Y02P20/10
Inventor 林陈胜张彦定
Owner FUJIAN NORMAL UNIV
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