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Application of combined resveratrol and dihydroartemisinin in treating liver cancer and breast cancer and product of combined resveratrol and dihydroartemisinin

A technology of dihydroartemisinin and resveratrol, applied in the field of medicine, can solve the problems of less than expected effect, complexity of anti-cancer mechanism, antagonism, etc.

Inactive Publication Date: 2019-04-12
SHANDONG NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the complexity of drug action mechanism and anti-cancer mechanism, the effect of some drugs in combination is not as good as expected, and even antagonism occurs.
At present, there is no report about resveratrol combined with dihydroartemisinin in the treatment of liver cancer and breast cancer

Method used

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  • Application of combined resveratrol and dihydroartemisinin in treating liver cancer and breast cancer and product of combined resveratrol and dihydroartemisinin
  • Application of combined resveratrol and dihydroartemisinin in treating liver cancer and breast cancer and product of combined resveratrol and dihydroartemisinin
  • Application of combined resveratrol and dihydroartemisinin in treating liver cancer and breast cancer and product of combined resveratrol and dihydroartemisinin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0063] Materials and Instruments:

[0064]

[0065]

[0066] 1. Effects of dihydroartemisinin and resveratrol alone and in combination on cancer cell proliferation

Embodiment approach

[0067] Implementation method: Liver cancer cell HepG2, human breast cancer MDA-MB-231 and human normal liver cell HL-7702 were inoculated in a 96-well plate, and the inoculation quantity was about 6000 cells / well. , 160 and 200 μM dihydroartemisinin, 50, 80, 110, 140 and 170 μM resveratrol, dihydroartemisinin: resveratrol: 10 μM: 20 μM, 25 μM: 50 μM, 40 μM: 80 μM, 40 μM: 20 μM, 40 μM: 50 μM and 10 μM: 80 μM and 20 μM: 80 μM were treated for 24 hours, and then MTT (3-(4,5-dimethylthiazole-2)-2,5-diphenyltetrazolium bromide) was added, Incubate in a 37°C incubator for 4 hours, remove the culture medium in the well, add 150 μl DMSO, shake on a shaker for 20 minutes, and use a microplate reader to detect the absorbance. Absorbance values ​​reflect cell viability. A blank control group and a DMSO control group were set without any drug intervention.

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PUM

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Abstract

The invention provides an application of combined resveratrol and dihydroartemisinin in treating liver cancer and breast cancer and a product of the combined resveratrol and dihydroartemisinin. When used in combination, the optimal drug concentration ratio of resveratrol to dihydroartemisinin is 25 [mu]M:50 [mu]M. Tests show that compared with separate drug usage groups, a combination drug usage group of the dihydroartemisinin and the resveratrol can more significantly inhibit the survival rate of liver cancer cells and breast cancer cells, and has less effect on normal cells; and when the IC50 is reached, the dosage of the combination drug usage group is lower than that of the separate drug usage groups so as to reduce the occurrence of adverse drug reactions, and to exhibit a good synergistic anti-tumor effect. Therefore, the product has a good practical application value.

Description

technical field [0001] The invention belongs to the technical field of medicine, and specifically relates to the application of resveratrol combined with dihydroartemisinin in the treatment of liver cancer and breast cancer and the product thereof. Background technique [0002] The information disclosed in this background section is only intended to increase the understanding of the general background of the present invention, and is not necessarily taken as an acknowledgment or any form of suggestion that the information constitutes the prior art already known to those skilled in the art. [0003] Primary liver cancer is currently one of the most common malignant tumors in the world, and its global incidence ranks fifth among neoplastic diseases. Liver cancer has a high degree of malignancy, low surgical resection rate, poor chemotherapy effect, and a 5-year survival rate of only 5% (Wagistri et al., 2017, Jeong et al., 2017). Breast cancer is one of the most common cancer...

Claims

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Application Information

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IPC IPC(8): A61K31/05A61K31/366A61P35/00
CPCA61K31/05A61K31/366A61P35/00A61K2300/00
Inventor 李国荣高俊英
Owner SHANDONG NORMAL UNIV