Method for extracting, separating and purifying lycium barbarum polysaccharide, lycium barbarum glycopeptide and preparation method thereof

Abstract

The invention belongs to the technical field of extraction, separation and purification; and discloses a method for extracting, separating and purifying lycium barbarum polysaccharide, lycium barbarumglycopeptide and a preparation method thereof. The lycium barbarum polysaccharide has the effects of enhancing immunity, preventing aging, enhancing hematopoiesis, preventing genetic damage and the like; the lycium barbarum polysaccharide (LBP) is a complex polysaccharide composed of acidic heteropolysaccharide and polypeptide or protein, and has glycopeptide structure of Glycano-O-Ser; the anti-tumor effect of the lycium barbarum glycopeptide is finished through extremely complex regulation and control modes: the apoptosis expression of tumor cells is induced; and the immunity of the body totumor cells is enhanced. The lycium barbarum polysaccharide can obviously improve the condition of tumor-bearing mice, prolong the life of the tumor-bearing mice with peritoneal effusion type by 41%-85%, and obviously inhibit the growth of the two types of solid tumors, and the inhibition rate reaches 81.0% and 72.9% respectively.

Description

technical field [0001] The invention belongs to the technical fields of extraction, separation and purification, and in particular relates to a method for extracting, separating and purifying wolfberry polysaccharide, wolfberry glycopeptide and a preparation method. Background technique [0002] At present, the prior art commonly used in the industry is like this: the nature and flavor of wolfberry fruit are sweet and flat; the nature and flavor of wolfberry leaves are bitter and sweet; cool in nature. Functionally, wolfberry can nourish the liver, nourish the kidney and nourish the lungs. Lycium barbarum leaves have the specific effect of tonifying deficiency and benefiting essence, clearing away heat and improving eyesight. Lycium barbarum polysaccharide is a water-soluble polysaccharide, which is the most important active ingredient in wolfberry, with a relative molecular mass of 68-200; Lycium barbarum polysaccharide is one of the main active ingredients in wolfberry, w...

AI Technical Summary

Problems solved by technology

Lycium barbarum polysaccharide is a water-soluble polysaccharide, which is the most important active ingredient in wolfberry, with a relative molecular mass of 68-200; Lycium barbarum polysaccharide is one of the main active ingredients in wolfberry, which has the functions of enhancing immunity, anti-aging, enhancing hematopoietic function, To prevent genetic damage and other effects, most of the prior art utilizes the crude product of Lycium barbarum polysaccharide; the purification method of the current existing technology is to use the traditional alcohol precipitation water extraction process, resulting in a yield of less than 1% for purified Lycium barbarum products, Moreover, the complex structure of the mixture cannot obtain a product that satisfies efficacy

[0004] (1) The purification methods of the existing technology all use the traditional alcohol precipitation and water extraction process, resulting in a yield of less than 1% for purified wolfberry products, and the structure of the mixture is complex, and products with satisfactory efficacy cannot be obtained;

[0005] (2) The process of the prior art is unstable, the structure of the product obtained each time is uncertain, the test results vary widely each time, and the yield is very low

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