Method for quickly extracting RNA, DNA or protein through animal and plant tissue ablation apparatus based on ultrasonic principle

Abstract

The invention discloses a method for quickly extracting RNA, DNA or protein through an animal and plant tissue ablation apparatus based on a ultrasonic principle. The method comprises the following steps of A, preparing cell suspension, and B, extracting RNA, DNA or protein, wherein the step A comprises the steps of mixing tissue samples with a solution, and performing ultrasonic ablation throughthe animal and plant tissue ablation apparatus based on the ultrasonic principle to obtain the cell suspension. The animal and plant tissue ablation apparatus based on the ultrasonic principle comprises a power supply, a DCDC conversion unit, a main control unit, a DCDC power regulating unit, a drive unit, a voltage transformation unit, a resonance unit, an ultrasonic vibrator and a sampling unit.According to the method disclosed by the invention, the animal and plant tissue ablation apparatus output adjustable power being 0.5-1000W and resonance ultrasonic frequency being 20-200KHz, a low-hertz ultrasonic method is utilized, the purpose of quickly and simply ablating tissue blocks into the cell suspension can be achieved, and subsequent extraction of tissue RNA, DNA or protein can be convenient; and the operation is simple, the extraction time is saved, human resources and material resources are saved, and the working efficiency can be improved.

Description

technical field [0001] The invention belongs to the technical field of RNA, DNA or protein extraction, and in particular relates to a method for rapidly extracting RNA, DNA or protein using an animal and plant tissue ablation instrument based on the principle of ultrasound. Background technique [0002] Ribonucleic acid (English: Ribonucleic acid, abbreviation: RNA) is a genetic information carrier that exists in biological cells and some viruses and viroids. A type of nucleic acid composed of at least dozens of ribonucleotides linked by phosphodiester bonds, named for its ribose sugar, referred to as RNA. Each RNA molecule is made up of long chains of nucleotide units, each containing a nitrogenous base, a ribose sugar, and a phosphate group. RNA is an intermediate carrier of genetic information in organisms with cell structure, and is involved in protein synthesis; it is also involved in the regulation of gene expression. For some viruses, RNA is the only genetic materia...

AI Technical Summary

Problems solved by technology

[0007] The above-mentioned conventional technology has the following defects: 1. It takes 10 minutes for a tissue to be homogenized from a tissue block to a tissue, which takes a long time; 2. Liquid nitrogen, a mortar and a tissue homogenizer are required during the operation, and the demand for consumables is large and difficult to operate. 3. If a large amount of tissue needs to be homogenized at one time, a large number of mortars and tissue homogenizers need to be sterilized in advance, and the workload is heavy; 4. The size of the ground tissue block should not be too small, so that the corresponding Trizol reagent and RIPA The amount of reagent added will also increase, resulting in waste of reagents

[0008] In order to improve the extraction efficiency and simplify the operation, the principle of a tissue crusher produced by American Thermo Company is to use the irregular high-speed movement of magnetic beads in the electromagnetic field to grind the tissue into a tissue homogenate, but the machine is expensive and can only homogenize the tissue. Slurry into smaller tissue clumps; the US PR0*PR0200 handheld and desktop dual-purpose homogenizer and Bioprep-24 biological sample homogenizer are high-throughput biological sample homogenizers, which also have expensive machines and can only homogenize tissues. pulp into smaller tissue clumps

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