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Annona squamosa seed extract for treating human brain malignant gliomas or melanoma and preparation method and application thereof

A technology for brain glioma and melanoma, applied in the field of custard apple seed extract and its preparation, can solve the problems of shortened survival period, decreased physical fitness and resistance of patients

Inactive Publication Date: 2019-04-16
INST OF MEDICINAL PLANT DEV CHINESE ACADEMY OF MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, chemotherapy drugs generally have the problem of excessive toxicity and side effects, which often lead to killing one thousand enemies and self-injury eight hundred, sharply reducing the patient's physical fitness and resistance, and greatly shortening the survival period

Method used

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  • Annona squamosa seed extract for treating human brain malignant gliomas or melanoma and preparation method and application thereof
  • Annona squamosa seed extract for treating human brain malignant gliomas or melanoma and preparation method and application thereof
  • Annona squamosa seed extract for treating human brain malignant gliomas or melanoma and preparation method and application thereof

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Experimental program
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preparation example Construction

[0045] The invention provides a kind of preparation method of custard apple seed extract, comprises the steps:

[0046] (1) pulverizing the custard apple seeds to obtain granules;

[0047] (2) mixing the particulate matter with a degreasing solvent, and separating the solid and liquid after degreasing to obtain a filter residue;

[0048] (3) mixing the filter residue with the extraction solvent, separating the solid and liquid after extraction to obtain a liquid phase;

[0049] (4) recovering the solvent in the liquid phase to obtain the annuus apple seed extract.

[0050] In the invention, the seeds of the custard apple are pulverized to obtain fine particles. In the present invention, the varieties of the custard apple seeds preferably include custard apple, soursop and / or custard apple. The particle size of the particles is preferably 2-25 mesh, more preferably 10-18 mesh.

[0051] After the particle is obtained, the present invention mixes the particle with a degreasin...

Embodiment 1

[0068] Sweet Apple, Ethanol Ultrasonic Extraction (Round-Ethanol Ultrasonic Extraction)

[0069] Take 20 grams of dried custard apple seeds, crush them into 15-mesh particles, add n-hexane to soak and degrease twice (use 200mL of n-hexane each time, shake intermittently, for 5 hours), filter with filter paper, and add 200ml of absolute ethanol to the filter residue , 25°C and 250W ultrasonic extraction twice, each time 30min, filter paper suction filtration, combined two filtrates, 55°C heating and rotary evaporation under reduced pressure to remove the organic solvent, vacuum drying to obtain the ethanol ultrasonic extract.

Embodiment 2

[0071] Annus apple, ultrasonically extracted with ethanol (soil-alcohol ultrasonically extracted)

[0072] Take 20 grams of dried custard apple (commonly known as custard apple) seeds, crush them into 18-mesh particles, add n-hexane to soak and degrease twice (use 200mL of n-hexane each time, shake intermittently, for 5 hours), filter paper suction, and filter residue Add 200ml of absolute ethanol, ultrasonically extract twice at 25°C and 250W for 30min each time, filter with filter paper, combine the two filtrates, heat at 60°C under reduced pressure and rotary evaporate to remove the organic solvent, and dry in vacuo to obtain the ethanol ultrasonic extract.

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Abstract

The invention provides application of compound of any one or more of a formula I, a formula II and a formula III for the preparation of a medicament for treating brain malignant gliomas or melanoma and belongs to the technical field of tumor medicines. The compound shown in the formula I, the formula II and the formula III is a monomer ingredient in an annona squamosa total lactones extract. The invention further provides a method for preparing an annona squamosa seed extract for treating human brain malignant gliomas or melanoma and a product thereof. The compound shown in the formula I, theformula II and the formula III and the extract (including effective parts) have an efficient killing effect on the brain malignant gliomas or melanoma. Particularly, the compound shown in the formulaI has an IC 50 value of only 0.4044 nM for brain malignant gliomas U87-MG, and an IC50 value of only 0.5152 nM for melanoma SK-MEL-28, while the IC50 for most other tumors is often between 1uM and 100uM, and the difference is more than three orders of magnitude, showing a selective and efficient killing effect on brain malignant gliomas U87-MG and melanoma SK-MEL-28.

Description

technical field [0001] The invention belongs to the technical field of tumor treatment and biomedicine, and in particular relates to an anemone apple seed extract for treating human malignant glioma, a preparation method and application thereof. Background technique [0002] Malignant tumor is a major disease that seriously endangers human life and health, and has become one of the main causes of death from diseases today. The incidence and mortality of cancer in my country continue to rise, and the situation is grim. At present, the main treatment methods for malignant tumors include surgery, radiotherapy and chemotherapy. Chemotherapy is still the main way to deal with tumors because the recurrence and metastasis of tumors after surgery still requires chemotherapy. Clinicians can optimize the maximum curative effect and minimum toxicity by adjusting the dosage of drugs, helping patients prolong survival time and improve the prognosis of life quality. However, chemothera...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/365A61P35/00C07D307/58
CPCA61K31/365A61P35/00C07D307/58
Inventor 王向涛敖惠李好文王一安郭一飞韩美华
Owner INST OF MEDICINAL PLANT DEV CHINESE ACADEMY OF MEDICAL SCI
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