Spiraea ultralow-temperature storage and culture method

A technology of cryopreservation and cultivation method, applied in the field of germplasm resource preservation, to achieve the effects of genetic stability, high recovery rate and regeneration rate, and good activity ability

Inactive Publication Date: 2019-04-19
GUANGXI BOTANICAL GARDEN OF MEDICINAL PLANTS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The cryopreservation technology of Spirae

Method used

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  • Spiraea ultralow-temperature storage and culture method

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Embodiment 1

[0028] The invention provides a method for ultra-low temperature preservation and cultivation of spirea, comprising: placing the spirea tip of 1 mm in length cultured in the pre-culture solution in a shaker shaker at room temperature and cultivating in the dark for 1 day, and then using a loading After 30 minutes of liquid treatment, the stem tip of Spiraea was taken out and transferred to a vitrification solution at 0°C for dehydration for 20 minutes, then put into a cryopreservation tube, and then the cryopreservation tube was stored in liquid nitrogen for ultra-low temperature preservation.

[0029] Wherein, the preculture solution is: 1 / 2MS+0.8mol / L sucrose+7g / L agar;

[0030] The loading solution is: 1 / 2MS+2mol / L glycerol+0.5mol / L sucrose

[0031] The vitrification solution is: PVS1

Embodiment 2

[0033] The invention provides a method for ultra-low temperature preservation and cultivation of Spiraea, comprising: placing the stem tip of Spiraea with a length of 2 mm in the pre-culture solution in a shaker shaker for 2 days in the dark for 2 days, and then using a loading After 40 minutes of liquid treatment, the stem tip of Spiraea was taken out and transferred to a vitrification solution at 0°C for dehydration for 40 minutes, then put into a cryopreservation tube, and then the cryopreservation tube was stored in liquid nitrogen for ultra-low temperature preservation.

[0034]Among them, the pre-culture solution is: MS+1.2mol / L glucose+0.2mg / mL NAA;

[0035] The loading solution is: 1 / 2MS+2mol / L glycerol+0.7mol / L sucrose

[0036] The vitrification solution is: PVS3

Embodiment 3

[0038] The invention provides a method for ultra-low temperature preservation and cultivation of Spiraea, which comprises: placing the stem tip of Spiraea with a length of 2 mm in the pre-culture solution in a shaker shaker for 3 days in the dark for 3 days, and then using a loading After 50 minutes of liquid treatment, the stem tip of Spiraea was taken out and transferred to a vitrification solution at 0°C for dehydration for 60 minutes, then put into a cryopreservation tube, and then the cryopreservation tube was stored in liquid nitrogen for ultra-low temperature preservation.

[0039] Among them, the pre-culture solution is: MS+1.2mol / L glucose+5%DMSO+1.0mg / mL6-BA

[0040] The loading solution is: 1 / 2MS+2mol / L glycerol+0.7mol / L sucrose

[0041] The vitrification solution is: PVS1

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Abstract

The invention discloses a spiraea ultralow-temperature storage and culture method The method comprises, at room temperature, placing a 1-2 mm long spiraea stem tip into a shaking table for culture indark for 1-3 d, processing the cultured spiraea stem tip with loading solution for 20-60 min, dewatering the processed spiraea stem tip in vitrification solution at 0 DEG C for 30-50 min and then placing the spiraea stem tip into a frozen storage pipe, and then storing the frozen storage pipe in liquid nitrogen for ultralow-temperature storage. Spiraea plants cultured through the spiraea ultralow-temperature storage and culture method can be high in recovery rate and regeneration rate. Therefore, the spiraea ultralow-temperature storage and culture method provides a technical guarantee of resource storage of endangered spiraea and an effective path for resource storage of rosaceous, spiraeoideae and spiraea plants as well as new species cultivation through plant diversity.

Description

technical field [0001] The invention relates to the technical field of germplasm resource preservation, in particular to a method for ultra-low temperature preservation and cultivation of spirea. Background technique [0002] Spiraea (Asparagus officinalis) is a perennial herb of the genus Asparagus in the family Liliaceae, also known as asparagus, asparagus, etc. According to literature reports, Spiraea contains rich minerals, amino acids, asparagus saponins, polysaccharides and flavonoids and other bioactive ingredients, which have pharmacological activities of anti-oxidation, anti-tumor, anti-fungal and hypolipidemic. Spiraea can clear away heat and dampness, promote blood circulation and dissipate stagnation, and treat mainly hepatitis, psoriasis, hyperlipidemia, and breast hyperplasia. It also has certain curative effects on lymphosarcoma, bladder cancer, breast cancer, and skin cancer. [0003] Cryopreservation (Cryopreservation) is a modern in vitro preservation tech...

Claims

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Application Information

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IPC IPC(8): A01H4/00A01N3/00
CPCA01H4/001A01H4/005A01N3/00
Inventor 李翠张占江缪剑华郭晓云
Owner GUANGXI BOTANICAL GARDEN OF MEDICINAL PLANTS
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