Method for preparing ganglioside through efficient hydrolysis conversion

A ganglioside, high-efficiency technology, applied in chemical instruments and methods, preparation of sugar derivatives, organic chemistry, etc., can solve problems such as short supply and achieve the effect of improving conversion rate

Inactive Publication Date: 2019-04-19
定安益尔生物技术有限公司
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Problems solved by technology

[0005] The object of the present invention is to provide a method for preparing gangliosides by efficient hydrolysis conversion, to solve the problem that the currently clinically applied monosialotetrahexosyl ganglioside sodium is mainly extracted from pig brain, and the extracted The rate is only about 3 / 10,000, and the clinical dosage forms are mainly injections, and the market has been in short supply for a long time

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  • Method for preparing ganglioside through efficient hydrolysis conversion

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Embodiment Construction

[0023] The method for preparing gangliosides by efficient hydrolysis conversion comprises the following steps:

[0024] Step 1: Pig brain crude extract extraction:

[0025] Extraction: Grind the fresh pig brain, add the extract solution (chloroform / methanol = 1:2) into the extraction tank at a ratio of 1:3 (w / v), heat at 40°C, stir and extract for 30 minutes, and let the layers stand for 40 minutes , filter press, collect supernatant and filtrate;

[0026] Stratification: add drinking water according to 25% of the volume of the extract, stir for 10 minutes, let stand for 12 hours to separate the layers, and collect the supernatant;

[0027] Saponification: add 0.3% of the volume of the supernatant to solid sodium hydroxide in a layered hydrolysis tank, control the temperature at 55-65°C, and stir for 30 minutes;

[0028] Resin chromatography: pass the hydrolyzate through the macroporous resin, wash the resin with 2 times the column volume of water, then wash the resin with 2...

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Abstract

The invention discloses a method for preparing ganglioside through efficient hydrolysis conversion. The method comprises the following steps of extracting a crude pig brain extract, performing silicagel chromatography purification on the crude pig brain extract, performing hydrolysis conversion, and carrying out silica gel chromatography purification on a crude GM1 product. The total gangliosidesilica gel is subjected to purification and separation to obtain most of GM1, the content of the separated crude GM1 product I reaches 80% or above, other ganglioside parts are hydrolyzed again, and the conversion rate is greatly increased.

Description

technical field [0001] The invention belongs to the technical field of ganglioside preparation, and in particular relates to a method for preparing ganglioside through efficient hydrolysis transformation. Background technique [0002] GM1 (sodium monosialotetrahexosyl ganglioside) is a safe and effective drug for neuroprotection and restoration, and has obvious curative effects on stroke, cerebral palsy, Parkinson's syndrome, etc. The conventional preparation process of total gangliosides is to directly hydrolyze the total gangliosides obtained by extracting the pig brain through an organic solvent, and the hydrolysis conversion rate is generally only about 2 times. This process first purifies and separates GM1 in the total gangliosides , and then hydrolyze other gangliosides, the hydrolysis conversion efficiency of gangliosides is significantly improved, the GM1 content is more than doubled through the new hydrolysis process, and the GM1 crude product obtained by this metho...

Claims

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Application Information

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IPC IPC(8): C07H15/10C07H1/08C07H1/00C07H1/06
CPCC07H1/00C07H1/06C07H1/08C07H15/10
Inventor 楚秋平陈雷谢智远
Owner 定安益尔生物技术有限公司
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