Receptor-transfected microvesicles, ligand-transfected microvesicles, and two-stage guided cell transfection methods for cell transfection

A receptor and microbubble technology, applied in the field of cell transfection, can solve the problems of low efficiency of lipid transfection, difficult to effectively achieve treatment, carcinogenesis, etc., and achieve the effect of avoiding risks such as carcinogenesis, low risk of carcinogenesis, and small side effects

Active Publication Date: 2022-04-05
PEKING UNIV SHENZHEN HOSPITAL
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Problems solved by technology

[0003] However, both of them have application defects. The former has low efficiency of lipofection and can only achieve a maximum transfection efficiency of 30%, making it difficult to effectively achieve the purpose of treatment. Although the latter has high efficiency of viral transfection, there are safety risks such as carcinogenicity.

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  • Receptor-transfected microvesicles, ligand-transfected microvesicles, and two-stage guided cell transfection methods for cell transfection
  • Receptor-transfected microvesicles, ligand-transfected microvesicles, and two-stage guided cell transfection methods for cell transfection
  • Receptor-transfected microvesicles, ligand-transfected microvesicles, and two-stage guided cell transfection methods for cell transfection

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Embodiment Construction

[0029] see figure 1 , the receptor transfection microvesicles for cell transfection provided in the preferred embodiment of the present application, structurally include receptor microvesicles 11, acceptor microvesicles 11 are coated with acceptor liposomes 12, and accept The body transfection microvesicles are lipid microvesicles of micron scale, and the acceptor liposome 12 is a positively charged liposome with a particle size of nanometer scale. Receptor liposome 12 passed through the artificial receptor R A Modification of the artificial receptor R A It is a polypeptide chain, and its amino acid sequence from N-terminal to C-terminal is: (KIAALKK) 3 .

[0030] see figure 2 , the ligand transfection microvesicles for cell transfection provided in the preferred embodiment of the present application structurally include ligand liposomes 21 and nucleic acid 22 to be transfected contained in the ligand liposomes 21 . Ligand liposome 21 is a positively charged liposome, an...

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Abstract

The application belongs to the technical field of cell transfection, and in particular relates to a receptor transfection microvesicle, a ligand transfection microvesicle and a two-stage guided cell transfection method for cell transfection. Receptor transfection microbubbles for cell transfection are formed by coating liposomes modified with artificial receptors with microbubbles, and ligand transfection microbubbles are formed by encapsulating nucleic acids with liposomes modified with artificial ligands. Carrier formation, a two-stage guided cell transfection method includes a receptor-cell structure formation step and a polypeptide-conjugated transfection step. This application performs cell transfection by simulating the way of virus membrane fusion instead of endocytosis, which has high transfection efficiency and low risk of carcinogenesis.

Description

technical field [0001] The application belongs to the technical field of cell transfection, and in particular relates to a receptor transfection microvesicle, a ligand transfection microvesicle and a two-stage guided cell transfection method for cell transfection. Background technique [0002] Gene therapy is the best way to finally solve the diseases caused by gene mutation, and the biggest difficulty of this way is to establish a gene transfection system. At present, the common transfection therapeutic vectors are divided into two categories: non-viral vectors and viral vectors; the non-viral vectors are mainly liposomes. [0003] However, both have application defects. The former has low efficiency of lipofection, and can only achieve a maximum transfection efficiency of 30%. . Therefore, the existing transfection technology needs to be improved accordingly. Contents of the invention [0004] In view of this, the present application provides receptor transfection mic...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/88A61K48/00
CPCA61K48/0025C12N15/88
Inventor 郑婷婷陈芸
Owner PEKING UNIV SHENZHEN HOSPITAL
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