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SNP (Single Nucleotide Polymorphism) marker remarkably related to Australian white sheep hoof color grade, molecular marker and application

A technology of molecular markers and sheep, which is applied in the fields of genetic engineering and molecular biology, can solve the problems of undiscovered patent documents and achieve the effect of improving selection efficiency

Active Publication Date: 2019-04-26
天津奥群牧业有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] Through the search of published patent documents, no published patent documents identical to this patent application have been found

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  • SNP (Single Nucleotide Polymorphism) marker remarkably related to Australian white sheep hoof color grade, molecular marker and application
  • SNP (Single Nucleotide Polymorphism) marker remarkably related to Australian white sheep hoof color grade, molecular marker and application
  • SNP (Single Nucleotide Polymorphism) marker remarkably related to Australian white sheep hoof color grade, molecular marker and application

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Embodiment Construction

[0024] Embodiments of the present invention are described in further detail below in conjunction with the accompanying drawings:

[0025] A SNP marker that is significantly correlated with hoof color of Australian white sheep. The innovation is that the marker has two alleles, A and G, and is located in the ninth intron of the MITF gene.

[0026] The SNP marker is located at the 31603203 nucleotide site on chromosome 19 of the International Sheep Genome Version 4.0 Reference Sequence, and has an A / G polymorphism, and the SNP marker is located at the ninth inclusion of the melanin production-related transcription factor MITF gene Among them, the SNP markers were extremely significantly correlated with hoof color of Australian white sheep P<0.01.

[0027] The one with A / G polymorphism is g.31603203A / G.

[0028] The hoof color of Australian white sheep with GG genotype at the g.31603203A / G site is gray, the hoof color of Australian white sheep with AG genotype at g.31603203A / G s...

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Abstract

The invention belongs to the field of animal genetics and breeding, SNP (Single Nucleotide Polymorphism) markers and application and in particular relates to an SNP marker remarkably related to an Australian white sheep hoof color grade, a molecular marker and application. The marker is located on a 31603203rd nucleotide site (Chr19:31603203) on a No. 19 chromosome of an international sheep genome(Oar_v4.0) and the site has two alleles A and G and a 9th intron located on an MITF (Microphthalmia-Associated Transcription Factor) gene; a g.31603203A / G site has A / G polymorphism; an Australian white sheep hoof color of a GG gene type is black; the Australian white sheep hoof color of an AG gene type is grey; the Australian white sheep hoof color of an AA gene type is white. The SNP molecular marker provided by the invention can be used for assisting the breeding of an Australian white sheep new strain with uniform appearance and stable properties and cultivating a new variety by utilizingthe Australian white sheep.

Description

technical field [0001] The invention belongs to the fields of genetic engineering and molecular biology, and relates to animal genetic breeding, SNP markers and applications, in particular to a SNP marker, molecular markers and applications significantly related to hoof color of Australian white sheep. Background technique [0002] Australian White Sheep is a coarse-haired sheep bred by the Australian White Sheep Breeders Association of Australia, which integrates the excellent genes of White-headed Dorper sheep, Wanrui sheep, Hornless Dorset sheep and Texel sheep. A specialized mutton sheep breed. This breed was officially registered in Australia in October 2009 and launched in March 2011. In May 2011, the Australian White sheep was introduced from the Australian HVD Breeding Company by Tianjin Aoqun Animal Husbandry Co., Ltd. In view of the problems encountered in the introduction and local domestication, after the introduction adaptability test is completed, combined wi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/124C12Q2600/156
Inventor 张清峰林春建陈华卢小芳杨漫漫李斌王然王斌佐建明刘莹
Owner 天津奥群牧业有限公司
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