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A protein crushing device

A technology for crushing equipment and proteins, which is applied in the fields of biochemical equipment and methods, biomass post-processing, peptide preparation methods, etc. Effect

Active Publication Date: 2021-12-07
WUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In order to determine the appropriate ultrasonic intensity and frequency, it is necessary to observe under the microscope at any time whether the bacteria are completely broken. Therefore, in the problem of protein breaking, it is necessary to break the air bubbles intermittently. This kind of operation method takes too long and leads to low efficiency. The problem

Method used

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  • A protein crushing device
  • A protein crushing device
  • A protein crushing device

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Embodiment Construction

[0028] The present invention will be further described below in conjunction with the specific embodiments in the accompanying drawings.

[0029] refer to Figure 1-7 , a kind of protein crushing equipment of the present invention, comprises cell crushing device and protein crushing device, wherein, protein crushing device comprises console 18, air pump 10, sealed tube 29, ultrasonic breaker 33 and test tube placement cylinder 34, console 18 and The air pump 10 is electrically connected, and the air pump 10 is a ventilation air source for blowing and sucking. connected, the other end is slidably inserted into the test tube placement cylinder 34, and the gas guide piston assembly connected to it is arranged on both sides of the push piston assembly, and the bubble mechanism is arranged on the periphery of the ultrasonic breaker 33 below the air guide piston assembly. There is a pushing assembly between the bubble rolling mechanism and the air guide piston assembly to push the b...

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Abstract

The invention discloses a protein crushing device, which includes a cell crushing device and a protein crushing device. The protein crushing device includes a console, an air pump, a sealed tube, an ultrasonic crusher and a test tube placement cylinder. The console and the air pump are electrically Connected, the air pump communicates with the sealing tube, one end of the ultrasonic breaker is slidingly connected with the sealing tube by pushing the piston assembly, and the other end is slidably inserted into the test tube placement cylinder, and the two sides of the pushing piston assembly are provided with connections to it. The air guide piston assembly, the air guide piston assembly is located on the periphery of the ultrasonic breaker and is provided with a foaming mechanism. . The protein breaking equipment of the present invention uses the equipment to break protein, so that the actions of protein breaking and soaking are carried out at the same time, the purpose of eliminating foam and steam drum in the test tube is realized, and the work efficiency is greatly improved.

Description

technical field [0001] The invention relates to the field of protein separation equipment, more specifically, it relates to a protein crushing equipment. Background technique [0002] When separating and extracting protein, ultrasonic crushing technology is generally used to crush the cells. During the crushing process, it is easy to cause the protein to not be completely broken, and the problems of air bubbles and foams are easy to appear. At this time, even if it is not possible to use uninterrupted stirring, It needs to be paused, then crushed and then paused, and operated in sequence to completely crush the protein after the bubbles disappear. Usually, try to crush for 8 seconds and stop for 8 seconds; Some, this is more common with proteins in the form of inclusion bodies. In order to determine the appropriate ultrasonic intensity and frequency, it is necessary to observe under the microscope at any time whether the bacteria are completely broken. Therefore, in the pro...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K1/14C12M1/00C12M1/36C12M1/33
Inventor 郑明周柚刘桂霞卓慕瑰
Owner WUZHOU UNIV
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