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A Breeding Method of Sweet and Waxy Double Hidden Maize Inbred Line with High Seedling Rate

A technology of inbred line and emergence rate, applied in botany equipment and methods, plant genetic improvement, application, etc., can solve the problems of insufficient nutrition and low germination rate, and achieve the effect of improving germination rate

Active Publication Date: 2020-10-13
HENAN ACAD OF AGRI SCI INST OF GRAIN CROPS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

When the wxwx gene is homozygous with the su1su1 and sh2sh2 genes, the development of the seed embryo is inhibited, and the seed embryo is too small, resulting in insufficient nutrition for the germination of the su1su1wxwx and sh2sh2wxwx double recessive inbred lines, and a low germination rate

Method used

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  • A Breeding Method of Sweet and Waxy Double Hidden Maize Inbred Line with High Seedling Rate
  • A Breeding Method of Sweet and Waxy Double Hidden Maize Inbred Line with High Seedling Rate

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] as per figure 1 Breeding is carried out according to the process shown:

[0034] ① Using waxy corn wxwxGeGeSh2Sh2 to cross with large embryo corn WxWxgegeSh2Sh2 to obtain F1 generation corn WxwxGegeSh2Sh2, using the F1 generation corn as the female parent;

[0035] ②Using super sweet corn WxWxGeGesh2sh2 to cross with large embryo corn WxWxgegeSh2Sh2 to obtain F1 generation corn WxWxGegeSh2sh2, using this F1 generation corn as the male parent;

[0036] ③ After crossing the above-mentioned female parent with the male parent, screen the maize Wx_Ge_Sh2_ showing small embryo traits in the F1 generation for self-crossing, and select the offspring maize WxWxgegesh2sh2 and Wxwxgegesh2sh2 with shrunken large embryos;

[0037]④Plant the large-embryo shrunken progeny corn, carry out iodine staining to the pollen, select the Wxwx genotype seeds with a pollen staining rate of 50% for selfing, and obtain 1 / 4 of WxWx gegesh2sh2, 1 / 2 of Wxwx gegesh2sh2 and 1 / 4 of Wxwx gegesh2sh2 and ...

Embodiment 2

[0041] according to figure 2 Breeding is carried out according to the process shown:

[0042] ① Crossing waxy maize wxwxGeGeSu1Su1 with large embryo maize WxWxgege Su1Su1 to obtain the F1 generation maize WxwxGegeSu1Su1, using the F1 generation maize as the female parent;

[0043] ②Use the super sweet corn WxWxGeGesu1su1 to cross with the large embryo corn WxWxgegeSu1Su1 to obtain the F1 generation corn WxWxGegeSu1su1, and use the F1 generation corn as the male parent;

[0044] ③ After crossing the above-mentioned female parent with the male parent, select the maize Wx_Ge_Su1_ showing small embryo traits in the F1 generation for self-crossing, and select the offspring maize WxWxgegesu1su1 and Wxwxgegesu1su1 with large embryo shrinkage;

[0045] ④Plant the large embryo shrunken progeny corn, carry out iodine staining to the pollen, select the Wxwx genotype seeds with pollen staining rate of 50% for selfing, and obtain 1 / 4 of WxWx gegesu1su1, 1 / 2 of Wxwx gegesu1su1 and 1 / 4 of ...

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Abstract

The invention provides a breeding method for a sweet-waxy recessive corn selfing line with a high emergence rate and relates to the technical field of corn breeding. The method comprises the followingsteps: hybridizing a waxy corn selfing line and a large embryo selfing line to obtain a single hybrid F1 as a female parent; then hybridizing a sweet corn selfing line and the single hybrid F1 obtained by hybridizing the waxy corn selfing line and the large embryo selfing line to obtain a double hybrid F1; screening large embryo seeds from the single double hybrid F1 and selfing the double hybridto obtain a generation F2; screening wrinkle phenotypic seeds of large embryo from the generation F2 and selecting a plant, the pollen iodine dyeing rate of which is 50%, to self to obtain a generation F3; selecting a plant, the pollen iodine dyeing rate of which is 100%, to self to obtain a generation F4; selecting head progeny rows, the emergence rate of which is greater than 80%, in the F4, and screening a large embryo sweet corn selfing line as a generation F5; and bagging the single F5 for continuous selfing for 3-4 generations to obtain the large embryo sweet-waxy selfing line. The emergence rate of the sweet-waxy recessive corn selfing line can be increased to 90% by means of the method.

Description

technical field [0001] The invention belongs to the technical field of corn breeding, and in particular relates to a method for breeding sweet and waxy double hidden corn inbred lines with high seedling emergence rate. Background technique [0002] Sweet and waxy corn is a new type of fresh corn variety that has both the nutrition and flavor of sweet and waxy corn bred by Chinese corn breeders in recent years (there are both waxy corn kernels and sweet corn kernels on the same ear, and the ratio of the two is 3: 1) It is deeply loved by Chinese consumers. There are already 3 million mu of planting area in my country, and a three-legged situation of waxy corn, sweet corn, and sweet plus waxy corn has quickly formed, and the market potential is very large. [0003] The basic condition for breeding sweet and waxy fresh corn varieties is that one of the parents of the hybrid must be a sweet and waxy double hidden inbred line, and the other parent must be a waxy corn inbred line. ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H1/02A01H1/04
Inventor 周波韩小花孙建军王延召鲁晓民刘康
Owner HENAN ACAD OF AGRI SCI INST OF GRAIN CROPS