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Biological tissue online culture device and observation method

A technology of biological tissue and culturing device, which is applied in medical detection device and biological field to achieve the effect of simple structure, low cost and low cost

Inactive Publication Date: 2019-05-10
金婧菲
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The technical task of the present invention is to provide a biological tissue online culture device and observation method to solve the problem of how to make slime molds, fungi and other similar biological cells can be cultured and observed online under a common microscope

Method used

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  • Biological tissue online culture device and observation method
  • Biological tissue online culture device and observation method
  • Biological tissue online culture device and observation method

Examples

Experimental program
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Effect test

Embodiment 1

[0046] as attached figure 1 and 2 Shown, the biological tissue online culture device of the present invention, its structure comprises concave glass slide 1 and cover glass 2, as attached image 3 As shown, the concave glass 1 is a disc-shaped structure with a groove 14 in the center, the cover glass 2 is located on the concave glass 1 and above the groove 14 of the concave glass 1, and the groove 14 of the concave glass 1 is in contact with the groove 14. The cover glass 2 forms a culture chamber 10; a liquid supply and drainage system is installed on the concave glass 1, and the liquid supply and drainage system includes a liquid supply subsystem and a liquid drainage subsystem, and the liquid supply subsystem and the liquid drainage subsystem are respectively located in the culture chamber 10. The two sides communicate with the culture chamber 10 respectively. The opposite sides of the culture chamber 10 are respectively provided with a culture chamber liquid inlet 7 and ...

Embodiment 2

[0049] as attached Figure 7 Shown, biological tissue online culture observation method of the present invention, the method steps are as follows:

[0050] (1), build the culture chamber 10 and put the test object into the culture chamber 10: put the biological tissue or cells to be tested into the groove 14 of the concave glass 1, drop an appropriate amount of solution and cover with the cover glass 2 ;

[0051] (2), set up a closed culture environment: after the air in the culture chamber 10 is discharged by dripping liquid into the culture chamber 10, the cover fragment 2 and the concave glass slide 1 are sealed with the sealant 9 and the liquid inlet of the culture chamber is reserved 7 and the drain port 8 of the culture chamber;

[0052] (3) Build a liquid supply and drainage system: build a liquid supply and drainage system according to the biological tissue online culture device according to any one of claims 1-8;

[0053] (4), adjust the liquid supply and discharge...

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Abstract

The invention discloses a biological tissue online culture device and observation method, and belongs to the technical field of biological detection. The technical problem to be solved is how to makemyxomycete, fungi and other similar biological cells subjected to online culture observation under a common microscope. According to the technical scheme, the biological tissue online culture device comprises a concave slide and cover glass, the concave slide is of a disc-shaped structure with a groove in the center, the cover glass is arranged on the concave slide and is located above the groovein the concave slide, and the groove in the concave slide and the cover glass form a culture cavity; a liquid supply and discharge system is arranged on the concave slide and comprises a liquid supplysubsystem and a liquid discharge subsystem, and the liquid supply subsystem and the liquid discharge subsystem are communicated with the culture cavity. The biological tissue online culture observation method comprises the following steps of 1, building the culture cavity; 2, building a closed culture environment; 3, establishing the liquid supply and drainage system; 4, adjusting the flow of supplied and discharged liquid; 5, injecting a nutrient solution and collecting waste liquid.

Description

technical field [0001] The invention relates to biological and medical detection devices and technical fields, in particular to an online biological tissue culture device and observation method. Background technique [0002] In the field of biology or medicine, the observation of tissue or cells is the most basic research method. The usual practice is to culture the tissue or cells to be observed in a culture vessel, and then transplant the tissue or cells to be tested onto a glass slide and examine them under a microscope. down for observation. However, limited by the high-power microscope stage, the distance between the objective lens and the glass slide, and the thickness of the glass cover glass, it is impossible to use special culture vessels for online culture and real-time observation under the microscope. However, the observation and recording of some organisms or tissue cells in the biomedical field requires long-term continuous observation and recording, which req...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M3/00C12M1/34C12M1/00
Inventor 金婧菲
Owner 金婧菲
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