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Endophytic fungi for reducing incidence rate of pseudo-ginseng root rot and microbial agent of endophytic fungi

An endophytic fungus and root rot technology, applied in the field of microorganisms, can solve problems such as difficulty in controlling pathogens

Active Publication Date: 2019-05-14
SHENYANG PHARMA UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the variety of pathogenic bacteria, it is difficult to control

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Acquisition of endophytic fungus Cladosporium tenuissimum SYPF 7101:

[0032] Collect healthy three-year-old Panax notoginseng plants, and the surface disinfection procedure is: 0.1Tween 20, soak for 1min → wash 3 times with sterile water → treat with sterile 2.5% sodium thiosulfate solution for 10 minutes → wash with sterile water → sterile 75% Ethanol treatment for 6min → wash with sterile water. The samples were cultured on the pulverized and coated PDA medium, and the strain SYPF 7101 was obtained.

Embodiment 2

[0034] The composition of the bacterial agent includes the notoginseng endophyte Cladosporium tenuissimum SYPF 7101: the preparation method is to prepare 50mL with a concentration of 1.0-5.0×10 6 The spore suspension per mL was inoculated into 400g solid substrate and cultured at 25-28°C. After 3-4 days of culture, it was stirred once under sterile conditions, and then cultured for 4-5 days after stirring to obtain solid fermenting bacteria. The obtained solid microbial agent is placed in a drying oven at 30-35°C, air-dried for 25-30 hours, packaged, and placed in a dry place.

Embodiment 3

[0035] Embodiment 3: the inoculant of embodiment 2 is to Radix Panax notoginseng root rot control effect

[0036] Select the land that has not been planted with Panax notoginseng and apply solid bacterial agent at the transplanting stage, the application rate is 0.5-1.0g / plant, a total of 120 seedlings, and use solid substrate or clear water as a control;

[0037] Bacterial agents are added, and after 3 months, an additional bacterial agent is added, and the additional amount is the same as above;

[0038] After 12 months of application, observe the occurrence of root rot of Panax notoginseng. The incidence of root rot in the control group is 55.8%, and the incidence of root rot in the treatment group is 20.8%. The results show that the incidence of root rot in the treatment group is lower than that in the control group. 35%.

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PUM

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Abstract

The invention belongs to the technical field of microorganisms, and relates to endophytic fungi for reducing the incidence rate of pseudo-ginseng root rot and a microbial agent of the endophytic fungi. The bacterial strain (SYPF 7101) is separated from the stem of biennials healthy pseudo-ginseng and identified as cladosporium tenuissimum, and the bacterial strain is preserved in the general microbiology center of China Committee for Culture Collection of Microorganisms, wherein the preservation number is CGMCC No.14126. The invention further provides a making method of a microbial agent containing the bacterial strain. The making method comprises the four steps of culture preparation, culture medium preparation, microbial agent production and microbial agent application. The microbial agent produced by using the method can significantly reduce the incidence rate of the pseudo-ginseng root rot and has a good ecological application prospect.

Description

Technical field: [0001] The invention belongs to the technical field of microbes, and relates to an endophytic fungus of the genus Cladosporium (Cladosporium tenuissimum) which can reduce the incidence of root rot of Panax notoginseng, and also relates to the preparation of a bacterial agent containing the fungus. Background technique: [0002] The root rot of Panax notoginseng is the most important disease in the growth process of Panax notoginseng. The annual yield reduction due to root rot is about 10% to 20%, and the serious one is more than 70%. Root rot has become an important factor affecting the yield of Panax notoginseng. Root rot of Panax notoginseng is caused by the infection of pathogenic microorganisms, including fungal pathogens Cylindrocarpon destructans, Cylindrocarpondidynum, Fusarium solani, Fusarium oxysporum, Fusarium moniliformVar.intermeclium, Phytophthora cactorum, Phoma herbarum, Alternaria panax, Alternaria tenuis and Rhizolanioctonia so (Research on...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A01N63/04A01P3/00C12R1/645
Inventor 张怡轩张天园章朦玥
Owner SHENYANG PHARMA UNIVERSITY
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