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Method for simultaneously extracting pectin and protein from sunflower plate

A sunflower plate and protein technology, applied in the preparation method of peptides, chemical instruments and methods, organic chemistry, etc., can solve the problems of protein loss of physical and chemical properties and physiological functions, protein denaturation, and inability to be effectively used, so as to save production energy Consumption and cost, corrosion reduction effect

Active Publication Date: 2019-05-17
JIANGNAN UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, under this condition, although pectin can be extracted, the protein in the sunflower cells will be denatured, causing the protein to lose its original physical and chemical properties and physiological functions, and high temperature and high acidity will promote the acid hydrolysis of the protein, resulting in its primary structure degradation
In addition, the residue after extracting pectin is strongly acidic, which is not even suitable for feed and cannot be effectively used

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Superfinely pulverize the dried sunflower discs to 100 mesh, then extract according to the ratio of solid to liquid 1:25, the dosage of sodium citrate is 8g / L, extract at 5°C for 2h, and the extraction pH is the natural pH of the raw material (6.2 ), filter after the reaction to get the pectin extract; then add absolute ethanol to the extract until the volume ratio of ethanol is 70% to precipitate the pectin, filter and dry to obtain the pectin; get the filtrate of the alcohol precipitation, vacuum concentration and recovery of ethanol , dry to obtain crude protein; under the above process, the pectin extraction rate is 14.3%, the pectin molecular weight is 450kDa, and the corresponding esterification degree is 42%; the protein extraction rate is 8.6%, and the molecular weight is 10kDa.

Embodiment 2

[0033] Superfinely pulverize the dried sunflower discs to 200 mesh, then extract according to the ratio of solid to liquid 1:30, the dosage of sodium citrate is 0.6% (to the mass volume ratio of the extract), extract at 20°C for 1 hour, and extract the pH It is the natural pH (6.0) of the feed liquid, after the reaction is finished, filter the pectin extract; then add absolute ethanol to the extract until the ethanol volume ratio is 85% to precipitate the pectin, filter and dry to obtain the pectin; The filtrate is vacuum-concentrated and reclaimed ethanol, and dried to obtain crude protein; under the above-mentioned process, the pectin extraction rate is 13.7%, and the pectin molecular weight is 10kDa, and the corresponding esterification degree is 21%; the protein extraction rate is 9.0%, and the molecular weight It is 9.4kDa.

Embodiment 3

[0035] Superfinely pulverize the dried sunflower discs to 300 mesh, then extract according to the ratio of solid to liquid 1:35, the amount of sodium citrate is 0.7% (to the mass volume ratio of the extract), extract at 40°C for 1 hour, and extract the pH It is the natural pH (6.0) of the feed liquid, after the reaction is finished, filter the pectin extract; then add absolute ethanol to the extract until the ethanol volume ratio is 90% to precipitate the pectin, filter and dry to obtain the pectin; The filtrate is vacuum-concentrated and reclaimed ethanol, and dried to obtain crude protein; under the above-mentioned process, the pectin extraction rate is 11.8%, and the pectin molecular weight is 7kDa, and the corresponding esterification degree is 12%; the protein extraction rate is 8.4%, and the molecular weight It is 9.6kDa.

[0036] The inventors have also carried out many explorations on the number of crushing meshes, and the results show that the extraction of pectin can...

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Abstract

The invention discloses a method for simultaneously extracting pectin and protein from a sunflower plate, and belongs to the technical field of agricultural product and by-product processing. The method for simultaneously extracting the pectin and the protein from the sunflower plate comprises the steps that the sunflower plate is smashed to 100-300 mesh, the extraction condition is controlled, sothat the pectin extraction rate in the sunflower plate reaches 10-15%, the pectin molecular weight is 7-500 kDa, and the corresponding degree of esterification is 45-10%; and the protein extraction rate is 8-10%, and the molecular weight is 5-20 kDa. Meanwhile, according to the method for simultaneously extracting the pectin and the protein from the sunflower plate, the situation that a traditional pectin extraction technology requires to adjust extraction solution pH to 1-2 strong acidity is avoided, the purpose of not using acid-base is achieved, corrosion of production equipment and pollution to the environment are reduced, and residual residues of extraction can be used biologically. The method for simultaneously extracting the pectin and the protein from the sunflower plate is further not required to be carried out at high temperatures of 80 DEG C and more than 80 DEG C, and production energy consumption and cost are saved.

Description

technical field [0001] The invention relates to a method for simultaneously extracting pectin and protein from sunflower discs, belonging to the technical field of agricultural by-product processing. Background technique [0002] Sunflower is a bulk oil crop, and the sunflower plate after removing sunflower seeds is a by-product that has not been fully exploited. Kuipan is rich in pectin (15-20%) and protein substances (10-15%), and its pectin is a natural low-methoxyl pectin, which is the only low-methoxyl pectin currently on the market. Pectin varieties, properties and uses are obviously different from high-ester pectins such as citrus and beet; its protein substances (including water-soluble proteins, peptides and amino acids) also have the physiological effect of reducing uric acid levels and treating gout. [0003] Pectin exists in the cell wall and intercellular layer of sunflower pan. The extraction of pectin generally requires high temperature (above 80°C), high aci...

Claims

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Application Information

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IPC IPC(8): C08B37/06C07K1/14
Inventor 华霄谭婧石晶白贵林刘敬然
Owner JIANGNAN UNIV