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Method for efficiently inhibiting photosynthetic bacteria phototaxis adherence for liquid culture of photosynthetic bacteria

A technology of photosynthetic bacteria and liquid culture, applied in the field of microbiology, can solve the problems of serious phototaxis and adherence, unfavorable large-scale and high-efficiency liquid culture, etc., and achieve the effect of low cost, favorable for large-scale and high-efficiency culture, and inhibition of adherent growth

Active Publication Date: 2019-05-17
HUAQIAO UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, during the light culture process, the bacteria have serious phototaxis and adherence to the wall, and the adherence rate is even as high as 70% of the total bacterial biomass in the reaction vessel, which is not conducive to large-scale and efficient liquid culture.

Method used

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  • Method for efficiently inhibiting photosynthetic bacteria phototaxis adherence for liquid culture of photosynthetic bacteria
  • Method for efficiently inhibiting photosynthetic bacteria phototaxis adherence for liquid culture of photosynthetic bacteria
  • Method for efficiently inhibiting photosynthetic bacteria phototaxis adherence for liquid culture of photosynthetic bacteria

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] Adopt formula 1 to add xanthan gum to prepare, refer to above-mentioned " containing 0.3% xanthan gum photosynthetic bacteria medium preparation " method, add reagent from formula 2 to formula 1, adjust the consumption of xanthan gum to prepare containing 0.05% (w / v), 0.25% (w / v), 0.35% (w / v) and 0.50% (w / v) xanthan gum purple sulfur bacteria culture medium, correspondingly divided into 500mL blue cap culture bottles (blue Cover culture bottles filled with liquid (the total volume is 530mL), each bottle is filled with 500mL medium, sterilized and cooled to room temperature; In the blue cap bottle, the inoculum amount accounts for 3.0% (v / v) of the total volume (530mL), and then fill up the blue cap culture bottle with the same medium, seal it with a cap, shake well, and its total volume is 530mL. Each treatment was repeated in 3 bottles, and the culture medium without xanthan gum (that is, the culture medium prepared in Formula 1) was used as a control.

[0051] Place...

Embodiment 2

[0054] Use formula 1 to add gelatin to prepare, with reference to the above-mentioned " containing 0.3% carrageenan photosynthetic bacteria culture medium preparation " method, fixation medium changes gelatin from carrageenan, adjusts the consumption preparation of gelatin containing 0.05% (w / v), 0.3% ( w / v) and the purple sulfur bacteria culture medium of 0.50% (w / v) gelatin;

[0055] Inoculate the suspension of marine pigmented bacteria YL28, and use the culture bottle without gelatin as the control. Inoculum size, culture temperature, time and light intensity are carried out according to the corresponding method of embodiment one, the mensuration of adherent biomass, suspended biomass and total biomass, and adherent rate (r%), suspension rate (r%) and The calculation method of the percentage (r%) of the total biomass is carried out according to the method described in Example 1.

[0056] Effects of gelatin on the adherence, suspension and total biomass of the marine chromo...

Embodiment 3

[0058] Formula 1 is prepared by adding carrageenan, referring to the method of "preparation of photosynthetic bacteria medium containing 0.3% carrageenan", adjusting the amount of carrageenan, and preparing a mixture containing 0.05% (w / v), 0.10% (w / v), 0.25% (w / v), 0.3% (w / v) and 0.50% (w / v) carrageenan culture medium for purple sulfur bacteria;

[0059] Inoculate the suspension of YL28 bacteria of marine pigmented bacteria, and use the culture bottle without carrageenan as the control. Inoculum size, culture temperature, time and light intensity are carried out according to the corresponding method of embodiment one, the mensuration of adherent biomass, suspended biomass and total biomass, and adherent rate (r%), suspension rate (r%) and The calculation method of the percentage (r%) of the total biomass is carried out according to the method described in Example 1.

[0060] Effects of carrageenan on the adherence, suspension and total biomass of the marine chromogener YL28 ...

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Abstract

The invention discloses a method for efficiently inhibiting photosynthetic bacteria phototaxis adherence for liquid culture of photosynthetic bacteria. An immobilized medium difficult to use in photosynthetic bacteria, good in dispersibility in liquid and medium mobile phase and free of toxicity and harm is added in the preparation process of a photosynthetic bacteria liquid medium. The immobilized medium comprises one or more of xanthan gum, carrageenan and gelatin, and then the medium is sterilized, inoculated and incubated. The xanthan gum, the carrageenan and the gelatin have good solubility characteristics and are dispersed in the photosynthetic bacteria medium to form a colloidal solution. The medium has good fluidity, and the photosynthetic bacterium thallus is adsorbed in the xanthan gum, the carrageenan and the gelatin in the culture process for suspended growth to inhibit the photosynthetic bacteria phototaxis adherence, and large-scale high-efficiency culture is facilitated.

Description

technical field [0001] The invention relates to the technical field of microbiology, in particular to a method for efficiently inhibiting photosynthetic bacteria from phototaxis and adherent liquid to cultivate photosynthetic bacteria in the production process of photosynthetic bacteria. Background technique [0002] At present, Anoxygenic Phototrophic Bacteria (APB) is a general term for a class of prokaryotic microorganisms that use various organic or inorganic substances as electron donors to perform photosynthesis without releasing oxygen under anaerobic conditions. "Photosynthetic bacteria (PSB)". [0003] Existing studies have shown that there are more than 80 genera and more than 200 species of photosynthetic bacteria. As a class of important microbial resources, photosynthetic bacteria are widely used in aquaculture (bait, bait additives and aquaculture water purifiers, etc.), agriculture (microbial fertilizers and plant growth) Regulators, etc.), animal husbandry (...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20
Inventor 杨素萍赵春贵周广静张晓波
Owner HUAQIAO UNIVERSITY
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