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Colloidal gold immunochromatography rapid detection card of insect-resistant protein Cry1C

A technology of insect-resistant protein and immune chromatography, applied in the field of bioengineering, can solve problems such as food toxicity, changes in food nutritional value, and allergens produced by genetically modified foods, and achieve the effects of simple operation, high sensitivity, and strong specificity

Active Publication Date: 2019-05-21
THE INST OF BIOTECHNOLOGY OF THE CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although genetically modified food can meet people's requirements for yield, insect resistance, etc., it also brings some potential threats to human life. , causing people to develop drug resistance, changing the nutritional value of food, etc.

Method used

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  • Colloidal gold immunochromatography rapid detection card of insect-resistant protein Cry1C
  • Colloidal gold immunochromatography rapid detection card of insect-resistant protein Cry1C
  • Colloidal gold immunochromatography rapid detection card of insect-resistant protein Cry1C

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Embodiment 1 detects transgenic rice leaf

[0034] Take 100 mg of transgenic rice leaves, grind with liquid nitrogen, fully mix with the sample extract, stand on ice for 30 minutes, and take 50 μL of the supernatant for testing. Observe the test results at room temperature for 5 minutes. For specific results, see image 3 ,pass image 3 It can be seen that the Cry1C protein contained in the stock solution can be specifically detected by the colloidal gold immunochromatography quick test card involved in the present application.

Embodiment 2

[0035] Example 2 Inspection of insect-resistant protein Cry1C colloidal gold immunochromatography quick test card

[0036] 1. Content:

[0037] 1.1 Preparatory work: (the sampling volume is not less than 20 copies, used for inspection; re-inspection, reserved samples and other special circumstances can be taken separately)

[0038] 1.1.1 Check, adjust, and approve the instruments or related equipment involved in the inspection item.

[0039] 1.1.2 Check whether the product name, batch number, batch and other information are consistent with the instructions;

[0040] 1.2 Appearance: Whether the components of the reagent (box) are complete, complete, and whether there is any leakage of liquid; whether the label is clear and easy to identify.

[0041] 1.3 Film strip width: Use a vernier caliper (with an accuracy of 0.02mm) to randomly test 2 strips of test paper, and calculate the average of the 2 test results. The result should meet the requirements of "Cry1C Colloidal Gold Fi...

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PUM

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Abstract

The invention discloses a colloidal gold immunochromatography rapid detection card for insect-resistant protein Cry1C, and belongs to the technical field of biological genes. The rapid test card comprises a card shell and a test strip in the card shell, wherein a sample adding hole and an observation window are formed in the card shell; the test strip comprises a bottom plate as well as a sample pad, a gold-labeled pad, a reaction membrane and a water absorption pad which are adhered to the bottom plate and are sequentially connected, the sample adding hole is formed above the sample pad, theobservation window is formed above the reaction membrane, a detection line and a quality control line are arranged on the reaction membrane, and the gold-labeled pad is coated with an insect-resistantprotein Cry1C monoclonal antibody-colloidal gold conjugate. The rapid detection card disclosed by the invention is strong in specificity, high in sensitivity, simple, convenient, rapid and accurate to operate, and suitable for on-site rapid detection and safety evaluation of the insect-resistant protein Cry1C.

Description

technical field [0001] The invention relates to the field of bioengineering, in particular to a colloidal gold immunochromatography quick test card for insect-resistant protein Cry1C. Background technique [0002] Bacillus thuringiensis (Bt) is a widespread Gram-positive bacterium, and the insect-resistant crystal protein secreted by the bacteria is currently the main biological pesticide; the secreted insect-resistant protein is divided into two types according to the similarity of amino acid sequence : Cry and Cry delta-endotoxin. Among them, the Cry protein is toxic to the larvae of various harmful insects (such as Lepidoptera, Diptera, Coleoptera, nematodes and protists, etc.). Cry toxins have been transferred to various crops to make them resistant to insects. Cry1C protein is a kind of Cry toxin. At present, the crops with Cry gene mainly include corn, potato, rice, cotton and so on. [0003] The development and progress of transgenic technology has promoted the de...

Claims

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Application Information

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IPC IPC(8): G01N33/68G01N33/58G01N33/553
Inventor 刘卫晓宛煜嵩高进金芜军苗朝华张哲
Owner THE INST OF BIOTECHNOLOGY OF THE CHINESE ACAD OF AGRI SCI