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Biotechnological production of alcohols and derivatives thereof

A technology of microorganisms and alkane oxidase, applied in biofuels, biochemical equipment and methods, enzymes, etc., can solve the problems of microbial growth, viability and metabolic disadvantages

Pending Publication Date: 2019-05-24
EVONIK DEGUSSA GMBH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Last, but not least, many of these, such as methanol and ethanol, are reactive and known to have adverse effects on the growth, viability and metabolism of microorganisms relevant to biotechnology

Method used

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  • Biotechnological production of alcohols and derivatives thereof
  • Biotechnological production of alcohols and derivatives thereof
  • Biotechnological production of alcohols and derivatives thereof

Examples

Experimental program
Comparison scheme
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Embodiment 1

[0061] Example 1: Accelerated Separation of Aqueous Medium from the Hydrophobic Phase Using Cells with Reduced Acyl CoA-Dehydrogenase Activity for the Production of ω-Aminolaurate Methyl Ester (ALSME)

[0062] Methyl laurate via ω-hydroxylauric acid to ω- Transformation of aminolauric acid (ALS) methyl ester.

[0063] Note that these two strains contain pBR322-derived plasmids containing the oxidoreductase AlkB, alcohol dehydrogenase and transaminase according to International Application WO 2009 / 077461, and in addition to the former being encoded in the acyl-CoA dehydrogenase of the E. coli β-oxidation pathway The two strains were identical except for a deletion in the gene for the enzyme FadE.

[0064] A 1 liter reaction vessel was used for the fermentation. The pH electrode was calibrated by two-point calibration using pH 4 and pH 7 standard solutions. Reactors containing 300 mL of tap water were autoclaved at 121 °C for 20 minutes. Subsequently, the pO2 detector was po...

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Abstract

The present invention relates to a microorganism having a reduced fatty acid degradation capacity and expressing a recombinant alkane oxidase, a method for oxidizing an alkyl, comprising a contactingthe alkyl with an aqueous solution comprising the inventive cell.

Description

[0001] This application is a divisional application of the Chinese patent application 201280063376.6 "Biotechnological Production of Alcohol and Its Derivatives" filed on December 14, 2012. technical field [0002] The present invention relates to microorganisms having reduced ability to degrade fatty acids and expressing a recombinant alkane oxidase; a method for oxidizing an alkyl group comprising contacting the alkyl group with an aqueous solution comprising cells of the invention. Background technique [0003] The industrial production of bulk and fine chemicals based on renewable resources such as biomass has a series of disadvantages. One of these is the need to extract the product of interest from large volumes of aqueous media, which necessitates not only concentrating the product for further downstream processing but also limiting its concentration in the aqueous media, which usually contains living cells. Such cells are extremely efficient but fragile catalysts tha...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/21C12N9/02C12P13/00C12P7/04C12P7/24C12P7/64
CPCC12N9/0077C12Y114/15003C12P7/04C12P7/24C12P7/6409C12P13/001C12N9/001C12N9/0071C12N9/1096C12Y114/11Y02E50/10C12P13/005
Inventor T.哈斯O.图姆J.C.普费弗P.恩格尔C.格林M.珀特
Owner EVONIK DEGUSSA GMBH