Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Hormone detection reagent and kit

A technology for detecting reagents and Mullerian hormones, which is applied in the field of anti-Mullerian hormone detection reagents, can solve the problems of difficulty in obtaining antibodies, high specificity, and restrictions on the development of AMH detection reagents, and achieves simple application and strong affinity , suitable for large-scale promotion and application

Inactive Publication Date: 2019-05-28
WUXI MATERNAL & CHILD HEALTH HOSPITAL +2
View PDF6 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Traditional AMH detection reagents are based on various immunological methods established by the double-antibody sandwich method, including enzyme-linked immunosorbent assay and chemiluminescence; however, AMH is a glycoprotein with a unique structure, which has high specificity and sensitivity. Good antibodies are very difficult to obtain; most of the AMH antibodies with excellent performance are monopolized by several foreign companies, which significantly limits the development of AMH detection reagents and technological progress

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1 Fluorescence-labeled WGA

[0028] Cleaning: Take fluorescent microspheres (purchased from Bangs Lab, product number: 11233) into a centrifuge tube, add 0.1M MES (2-morpholinoethanesulfonic acid) (pH 5.0) buffer and mix well, and run at 13000rpm for 15min, Centrifuge at 4°C, discard the supernatant, and resuspend with 0.1MMES (pH 5.0) buffer for later use. Activation and cleaning: the activator EDC (1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride), NHS (N-hydroxysuccinimide) and fluorescent microspheres according to the mass The ratio is 2:1:2 for activation, the specific operation is as follows:

[0029] Weigh EDC and NHS and add them to 0.1M MES (pH 5.0) buffer solution to dissolve, quickly take an appropriate amount into the washed fluorescent microspheres, seal them with a parafilm and place them on a 200rpm shaker at room temperature for 30min, take them out at 13000rpm for 30min , centrifuged at 4°C to remove the supernatant, resuspended wi...

Embodiment 2

[0035] Example 2 Fluorescently labeled AMH antibody

[0036] Cleaning: Take fluorescent microspheres (purchased from Bangs Lab, product number: 11233) into a centrifuge tube, add 0.1M MES (pH5.0) buffer, mix well, and centrifuge at 13000rpm, 15min, 4°C, discard the supernatant, Resuspend with 0.1M MES (pH 5.0) buffer for use. Activation and cleaning: Activate the activator EDC, NHS and fluorescent microspheres according to the mass ratio of 2:1:2, the specific operation is as follows:

[0037] Weigh EDC and NHS and add them to 0.1M MES (pH 5.0) buffer solution to dissolve, quickly take an appropriate amount into the washed fluorescent microspheres, seal them with a parafilm and place them on a 200rpm shaker at room temperature for 30min, take them out at 13000rpm for 30min , centrifuged at 4°C to remove the supernatant, resuspended with an equal amount of 0.1M MES (pH 6.5) buffer solution, ultrasonically mixed and washed, repeating the above operation once, that is, washing...

Embodiment 3

[0043] Example 3 Fluorescence-labeled goat anti-rabbit IgG antibody

[0044] Cleaning: Take fluorescent microspheres (purchased from Bangs Lab, product number: 11233) into a centrifuge tube, add 0.1M MES (pH5.0) buffer, mix well, and centrifuge at 13000rpm, 15min, 4°C, discard the supernatant, Resuspend with 0.1M MES (pH 5.0) buffer for use. Activation and cleaning: Activate the activator EDC, NHS and fluorescent microspheres according to the mass ratio of 2:1:2, the specific operation is as follows:

[0045] Weigh EDC and NHS and add them to 0.1M MES (pH 5.0) buffer solution to dissolve, quickly take an appropriate amount into the washed fluorescent microspheres, seal them with a parafilm and place them on a 200rpm shaker at room temperature for 30min, take them out at 13000rpm for 30min , centrifuged at 4°C to remove the supernatant, resuspended with an equal amount of 0.1M MES (pH 6.5) buffer solution, ultrasonically mixed and washed, repeating the above operation once, ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
concentrationaaaaaaaaaa
Sensitivityaaaaaaaaaa
Sensitivityaaaaaaaaaa
Login to View More

Abstract

The invention relates to an anti-mullerian hormone (AMH) detection reagent. The detection reagent comprises an anti-mullerian hormone antibody, wheat germ agglutinin and a solid-phase carrier, the solid-phase carrier is coated with one of the anti-mullerian hormone antibody and the wheat germ agglutinin, and the other one of the anti-mullerian hormone antibody and the wheat germ agglutinin is marked with a marker. The detection reagent is mainly characterized by being formed from the anti-AMH antibody and the wheat germ agglutinin (WGA) through a sandwich method. The detection reagent is highin detection sensitivity, high in specificity and suitable for large-scale popularization.

Description

technical field [0001] The patent of the present invention relates to the technical field of detection reagents, in particular to the field of detection reagents, in particular to a detection reagent for anti-Müllerian hormone (AMH). Background technique [0002] Women's reproductive ability is related to age, basic follicle stimulating hormone, inhibin B (inhibin B, INH B), anti-Mullerian hormone (anti mμLlerian hormone, AMH), estradiol, antral follicle number and other factors. Among them, AMH is the most important indicator. [0003] AMH is a glycoprotein dimer composed of two 72kD single chains connected by a disulfide bridge, belonging to a member of the β-transforming factor family; AMH is secreted by immature Sertoli cells of the testis and granulosa cells of postnatal ovarian growth follicles , has the function of regulating cell development and differentiation, and degenerating the Mullerian duct of male embryos. In women, AMH is produced by follicular granulosa c...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/53G01N33/543G01N33/577G01N33/533
Inventor 臧嘉许耀辉王晶陈妮娜贾梦玥张玲杨海麟
Owner WUXI MATERNAL & CHILD HEALTH HOSPITAL
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com