Method for determining multi-component content of Codonopsis pilosula based on HPLC multi-wavelength superposition

A determination method and multi-component technology, which is applied in the direction of measuring devices, material separation, and analysis of materials, can solve the problems of time-consuming, consumption of Codonopsis pilosula reagents, and failure to simultaneously determine the content of salvia miltiorrhiza in order to achieve stable test results, save time and cost effect

Inactive Publication Date: 2019-06-04
ZUNYI MEDICAL UNIVERSITY
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  • Application Information

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Problems solved by technology

[0003] In the prior art, for the determination of the contents of Codonopsis Codonopsis Codonopsis glucoside, syringin, Atractylodes lactone I, Atractylodes lactone II and Atractylodes lactone III, the content of two components can only be determined simultaneously at most.
When it is necessary to measure multiple components of Cod...

Method used

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  • Method for determining multi-component content of Codonopsis pilosula based on HPLC multi-wavelength superposition
  • Method for determining multi-component content of Codonopsis pilosula based on HPLC multi-wavelength superposition
  • Method for determining multi-component content of Codonopsis pilosula based on HPLC multi-wavelength superposition

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Embodiment Construction

[0017] Further detailed explanation through specific implementation mode below:

[0018] Embodiment: a kind of Codonopsis Radix multicomponent content assay method based on HPLC multi-wavelength superposition, measures according to the following steps:

[0019] Step 1. Set chromatographic conditions: the instrument used is Agilent 1260 high performance liquid chromatograph, KQ~500DV digitally controlled ultrasonic cleaner, RATANKVN1 ultrapure water instrument; the chromatographic column is Inertsil ODS~3, the specification is 4.6mm×250mm, 5μm ; With acetonitrile (chromatographic grade) as mobile phase A, water as mobile phase B, carry out gradient elution according to the program in Table 1, the flow rate is 0.8mL / min, the column temperature is 30 ° C, and the detection wavelength is 220nm, 267nm, 276nm;

[0020] Table 1:

[0021] time / min

Mobile phase A / %

Mobile phase B / %

0

96.5

3.5

3

96.5

3.5

20

86

14

35

77

2...

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Abstract

The invention discloses a method for determining multi-component content of Codonopsis pilosula based on HPLC multi-wavelength superposition in the technical field of traditional Chinese medicine detection. Lobetyolin, syringin, atractylenolide I, atractylenolide II and atractylodesin III are used as reference substances. Acetonitrile is used as mobile phase A, and water is used as mobile phase B.According to a gradient elution procedure, from 0 to 3min, the volume ratio of acetonitrile to water is 96.5:3.5; at 20min, the volume ratio of acetonitrile to water is 86:14; at 35min, the volume ratio of acetonitrile to water is 77:23; at 60min, the volume ratio of acetonitrile to water is 28:72; and at 76min, the volume ratio of acetonitrile to water is 5:95 by high performance liquid chromatography. The determining method provided by the invention can determine the content of various components at one time, which not only saves time and cost, but also makes a test result more stable and reliable under the same system condition.

Description

technical field [0001] The invention belongs to the technical field of traditional Chinese medicine detection, and in particular relates to a multi-component content determination method of Codonopsis pilosula based on HPLC multi-wavelength superposition. Background technique [0002] Codonopsis Codonopsis is the root of the perennial herb Codonopsis Codonopsis, Suhua Codonopsis, Sichuan Codonopsis and other plants of the same genus. Codonopsis Codonopsis is a traditional tonic medicine commonly used in China. In ancient times, Codonopsis Codonopsis produced in Shangdang area of ​​Shanxi was the top grade. It has the effects of nourishing the middle and Qi, strengthening the spleen and lungs. Modern research shows that Codonopsis pilosula contains various sugars, phenols, sterols, volatile oils, baicalein glucoside, saponins and trace alkaloids, which have the functions of enhancing immunity, dilating blood vessels, lowering blood pressure, improving microcirculation, and en...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06
Inventor 吴发明刘付松王道清刘莎陈翠莎
Owner ZUNYI MEDICAL UNIVERSITY
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