Device for on-line detection of water-quality biotoxicity

A biological toxicity, water quality technology, applied in the direction of chemiluminescence/bioluminescence, analysis by making materials undergo chemical reactions, etc.

Inactive Publication Date: 2019-06-07
廊坊市慧璟科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to overcome the existing problems and defects of the existing technology and products, and to provide an on-line detection device and detection method for biotoxicity of luminescent bacteria that can be continuously and stably operated, which is suitable for online monitoring of various complex water bodies

Method used

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  • Device for on-line detection of water-quality biotoxicity
  • Device for on-line detection of water-quality biotoxicity
  • Device for on-line detection of water-quality biotoxicity

Examples

Experimental program
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Embodiment 1

[0024] Example 1: Preservation and Recovery of Luminescent Bacteria

[0025] One of the key technologies of the water quality biological toxicity online detection device is how to store the luminescent bacteria stably in the instrument for a long time, and how to quickly recover the bacterial performance when it needs to be used, so as to ensure the stability and reliability of the continuous monitoring of the instrument. In order to preserve the luminescent bacteria, the device includes a bacterial liquid storage pool 101 containing temperature control and magnetic stirring. The temperature of the pool 101 is adjusted and stabilized to 5±0.5°C. At this temperature, the bacteria metabolize slowly and can be stored for more than 10 days. In addition, a magnetic stirrer is set in the bacterial liquid storage pool 101, and the bacterial liquid is heated at a speed of 200 rpm. Oxygenation is carried out to ensure the oxygen concentration required for the survival of luminescent ba...

Embodiment 2

[0026] Embodiment 2: the continuous measurement of water sample

[0027] Using the water quality biological toxicity online detection device involved in this patent, the typical process of continuous measurement of actual water samples mainly includes the following steps: ①Initialization - pump salt solution, pure water, and water samples into the corresponding salt solution pool 105. In the pure water pool 106 and the water sample pool 107; ②Recovery culture of luminescent bacteria—mix 3000 μL pure water, 300 μL salt solution, and 100 μL bacterial solution into the bacterial solution recovery tank 102; ③The water sample to be tested and the reference water sample Preparation——mix 3100 μL of water samples and pure water with 300 μL of saline solution into the reaction pools 103 and 104; ④ draw the liquid in the bacterial recovery pool 102 into the syringe and measure the luminance of the two channels; The liquid in the liquid resuscitation pool 102 is transferred to the reacti...

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Abstract

Provided in the invention is a device for on-line detection of water-quality biotoxicity. The device comprises a sample preparation unit including a water sample pretreatment unit, a mechanical arm unit, a sampling detection unit and a drive analysis unit. The sample preparation unit and the sampling detection unit employ the parallel two-channel technical solution to realize simultaneous measurement of a reference water sample and a to-be-detected water sample and eliminate a system error and a measurement deviation. The device has advantages of simple operation, great convenience in maintenance, high automation degree, flexible configuration and the like and is especially suitable for real-time online water-quality toxicity detection of the surface water, the drinking source water, and various waste water.

Description

technical field [0001] The invention relates to an online measurement device and detection method for water quality toxicity evaluation based on the toxicity response of luminescent bacteria. The application field includes real-time online monitoring of water quality biological toxicity of various natural water bodies and various sewage, and belongs to the field of environmental monitoring. Background technique [0002] Most of the current traditional analysis methods for water environmental pollutants are chemical analysis, which is time-consuming and labor-intensive, and because various chemical substances may have antagonism or synergistic effects with each other, the toxicity of the sample cannot be directly determined; in addition, substances harmful to the human body that exist in the natural environment There are tens of thousands of species, and the pollutants specified in all environmental standards are limited, and it is impossible to detect the concentration / conten...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/76
Inventor 赵东红
Owner 廊坊市慧璟科技有限公司
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