Preparation method and application of autologous tumor vaccine

A tumor vaccine, autologous technology, applied in the fields of biotechnology and immunology, can solve the problems of lack of immunogenicity and specific autologous tumor vaccine preparation methods

Inactive Publication Date: 2019-06-14
XIAMEN LUJIA BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] At present, there is still a lack of good immunogenicity and specific autologous tumor vaccines and their preparation methods, and there is an urgent need to develop corresponding methods and vaccines in this field

Method used

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  • Preparation method and application of autologous tumor vaccine
  • Preparation method and application of autologous tumor vaccine
  • Preparation method and application of autologous tumor vaccine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0106] Preparation of individualized tumor vaccines

[0107] Take 4T1 cells or Lewis lung cancer cells in logarithmic growth phase 1*10 7 After being irradiated with γ-rays (4-10Gy, 1.0Gy / min) for 30-60 minutes, heat in a water bath at 37-40°C for 20 minutes; at 37°C, 5% CO 2 After 48 hours in a constant temperature incubator, the cells were disrupted by ultrasonic waves, and Freund's adjuvant was added to prepare an emulsified agent, which is the tumor whole cell vaccine.

Embodiment 2

[0109] Individualized tumor vaccine for the treatment of subcutaneous breast cancer tumors in mice

[0110] In this example, the relevant indicators of cell and animal experiments confirmed that the tumor vaccine prepared by the method of the present invention has strong antigen presentation and the ability to stimulate antibody immune response. The specific results are as follows:

[0111] 1. Promote the production of anti-tumor antibodies

[0112] Experimental method: 3 weeks after conventional Balb / c mice were immunized with the tumor cell antigen prepared in Example 1, the plasma was taken and diluted, and added to an ELISA plate coated with tumor cells to resist mouse IgG-HRP and TMB substrate Display the antibody titer (1:100000 in this experiment).

[0113] The results show that compared with the tumor vaccine prepared by the control method, the tumor antigen prepared in Example 1 has a stronger ability to promote the production of anti-tumor antibodies ( figure 1 ):...

Embodiment 3

[0118] Inhibitory effect on tumors in mice

[0119] According to the principles of immunology, the mouse anti-tumor immunity test is divided into two steps:

[0120] 1. Tumor immune sensitization: inoculate the treated tumor antigen new tumor vaccine in the subcutaneous area of ​​the mouse to be tested, and boost the immunization once after 14 days, and inoculate the homologous mouse tumor cells ( image 3 ).

[0121] 2. Immune anti-cancer effect: divided into two categories:

[0122] a. Inhibitory effect on carcinoma in situ: using BALB / c mice and 4T1 breast cancer cell lines;

[0123] b. Inhibitory effect on metastatic tumors: C57BL / 6 mice and Lewis lung cancer cell lines were used. Both Lewis and 4T1 cancer cells are highly malignant, fast-growing, difficult to control, and capable of forming lung metastases.

[0124] Judgment of anti-tumor effect:

[0125] 1. Judgment of curative effect on antigenic carcinoma:

[0126] a. In vivo tumor fluorescence quantification met...

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Abstract

The invention relates to a preparation method and application of an autologous tumor vaccine. The method comprises the following specific steps: radiating and / or heating autologous tumor cells to greatly excite the antigenicity of the autologous tumor cells and enhance the presentation property of the antigen by using heat shock proteins; and inactivating the tumor cells and adding an adjuvant toobtain the vaccine. The vaccine prepared by using the method has the special advantages of high presentation property and high tumor immunogenicity, and can be used for greatly exciting the body fluidand cellular immune response of tumor patients to autologous tumors, monitoring immune response, and timely adjusting a usage method of the novel vaccine and adjuvant treatment, so that the aims of improving the antigenicity and metastatic tumor effect and prolonging the survival time of tumor patients are fulfilled.

Description

technical field [0001] The invention belongs to the field of biotechnology and immunology, in particular, the invention relates to a preparation method and application of an autologous tumor vaccine. Background technique [0002] Tumor is currently one of the main causes of death in humans. Although the level of diagnosis and treatment of tumors has been continuously improved and improved in recent years, and the regimens of chemotherapy and radiotherapy have also been continuously improved, but in the end most patients still cannot escape the bad luck of death. [0003] Among the different treatment modes of malignant tumors, immunotherapy has attracted more and more attention because of its specific killing of tumor cells, high efficiency and low side effects. In recent years, advances in molecular biology and further understanding of the function of the immune system have led to a rapid development of research and development of biotherapeutic methods. The development o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/00A61P35/00A61P35/04
CPCA61K39/0011A61K2039/5152A61P35/00A61P35/04
Inventor 韩德平林建华张鲁榕张美张震寰张亨山吕文龙洪金省
Owner XIAMEN LUJIA BIOTECH
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