Aquatic probiotic agent as well as preparation method and application thereof

Abstract

The invention relates to an aquatic probiotic agent. The aquatic probiotic agent comprises rhodopseudomonas palustris HEW-GJ106 which is collected in CGMCC (China General Microbiological Culture Collection Center) with the collection number of CGMCC NO.17021. The aquatic probiotic agent also comprises one or more of enterococcus faecalis, lactobacillus plantarum, pediococcus acidilactici, bacillussubtilis or saccharomyces cerevisiae. The invention also provides a preparation method and an application of the aquatic probiotic agent. The probiotic agent can improve the utilization efficiency ofaquatic animals, the cost is saved, the steady state of the internal environment of digestive tracts in animal bodies is greatly improved, nutrition absorption and utilization are promoted, animal growth is promoted, and the production performance, the immunity performance and the stress resistance of animals are notably improved.

Description

technical field

[0001] The invention relates to a probiotic agent, in particular to an aquatic probiotic agent and its preparation method and application, more specifically to an aquatic probiotic agent containing Rhodopseudomonas palustris and its preparation method and application. Background technique

[0002] The positive effect of probiotics as feed additives has been recognized, but there are still problems to be solved urgently: (1) The currently recognized strains suitable for probiotics in China are only Lactobacillus, Streptococcus, Bacillus, Bifidobacterium Bacillus and yeast etc. 12 kinds; (2) live bacteria preparations are easy to lose activity during feed processing, transportation and storage, and reduce biological activity; (3) live bacteria preparations are difficult to withstand low pH value after entering the animal digestive tract Due to the effects of hydrochloric acid, bile acid, etc., it is difficult to have enough viable bacteria to reach the intestin...

Examples

Embodiment 1

[0087] The preparation of each strain fermented liquid of embodiment 1

[0088] ⑴ Preparation of Rhodopseudomonas paluscans fermentation broth

[0089] After thawing Rhodopseudomonas palustris HEW-GJ106 frozen at -20°C, streak it on the photosynthetic bacteria medium under sterile conditions, culture it for 4 days at 30°C with a light intensity of 3500LX, and obtain Rhodopseudomonas palustris Pick a single colony of bacteria HEW-GJ106, pick a single colony in the seed medium, and cultivate it for 4 days at 30°C with a light intensity of 3500LX to obtain a seed solution of Rhodopseudomonas palustris HEW-GJ106, with an activity of 10 9 CFU / mL.

[0090] Rhodopseudomonas palustris (Rhodopseudomonas palustris) HEW-GJ106 provided by the present invention has the following microbiological characteristics: Rhodopseudomonas palustris HEW-GJ106 is a Gram-negative bacterium, grows rapidly on photosynthetic bacteria culture medium, 30 ℃ The colony formed by light culture for 4 days is s...

Embodiment 2

[0132] The preparation of embodiment 2 aquatic probiotics

[0133] Centrifuge the fermentation broth of Rhodopseudomonas palustris, Enterococcus faecalis, Lactobacillus plantarum, Pediococcus lactis, Bacillus subtilis and Saccharomyces cerevisiae (8000r / m, the time is different according to the amount of fermentation broth, generally A batch of sludge can be produced in 40 minutes) to obtain active sludge of various strains. Put the active bacteria slime of each strain and the stabilizing protective agent in the mixing tank according to the mass ratio (0.5-3):(1-4), start the mixer, mix evenly for 25min, the stirring speed is 40r / m, and get the bacteria wet powder, and then the bacteria wet powder is frozen, and then freeze-dried to obtain probiotic powders of various strains.

[0134] The stabilized protective agent is prepared by uniformly mixing raw materials by weight percentage: 1-10% corn starch; 1-5% microcrystalline cellulose; 1-15% sodium alginate; 1-15% sodium carbo...

Embodiment 3

[0143] The preparation of embodiment 3 aquatic probiotics

[0144] The preparation of each bacterial agent of Rhodopseudomonas palustris, Enterococcus faecalis, Lactobacillus plantarum, Pediococcus lactis, Bacillus subtilis and Saccharomyces cerevisiae is the same as that in Example 2, and the aquatic probiotic agent is to combine Rhodopseudomonas palustris, feces Enterococcus, Lactobacillus plantarum, Pediococcus lactis, Bacillus subtilis, Saccharomyces cerevisiae inoculum and auxiliary materials are uniformly mixed according to the weight ratio of 2:1.2:2.1:0.8:3.2:1:2, and the activity is 50 billion CFU / g.

[0145] The percentage by weight of each component of described adjuvant is the same as in Example 2.

technical field

[0001] The invention relates to a probiotic agent, in particular to an aquatic probiotic agent and its preparation method and application, more specifically to an aquatic probiotic agent containing Rhodopseudomonas palustris and its preparation method and application. Background technique

[0002] The positive effect of probiotics as feed additives has been recognized, but there are still problems to be solved urgently: (1) The currently recognized strains suitable for probiotics in China are only Lactobacillus, Streptococcus, Bacillus, Bifidobacterium Bacillus and yeast etc. 12 kinds; (2) live bacteria preparations are easy to lose activity during feed processing, transportation and storage, and reduce biological activity; (3) live bacteria preparations are difficult to withstand low pH value after entering the animal digestive tract Due to the effects of hydrochloric acid, bile acid, etc., it is difficult to have enough viable bacteria to reach the intestin...

Examples

Embodiment 1

[0087] The preparation of each strain fermented liquid of embodiment 1

[0088] ⑴ Preparation of Rhodopseudomonas paluscans fermentation broth

[0089] After thawing Rhodopseudomonas palustris HEW-GJ106 frozen at -20°C, streak it on the photosynthetic bacteria medium under sterile conditions, culture it for 4 days at 30°C with a light intensity of 3500LX, and obtain Rhodopseudomonas palustris Pick a single colony of bacteria HEW-GJ106, pick a single colony in the seed medium, and cultivate it for 4 days at 30°C with a light intensity of 3500LX to obtain a seed solution of Rhodopseudomonas palustris HEW-GJ106, with an activity of 10 9 CFU / mL.

[0090] Rhodopseudomonas palustris (Rhodopseudomonas palustris) HEW-GJ106 provided by the present invention has the following microbiological characteristics: Rhodopseudomonas palustris HEW-GJ106 is a Gram-negative bacterium, grows rapidly on photosynthetic bacteria culture medium, 30 ℃ The colony formed by light culture for 4 days is s...

Embodiment 2

[0132] The preparation of embodiment 2 aquatic probiotics

[0133] Centrifuge the fermentation broth of Rhodopseudomonas palustris, Enterococcus faecalis, Lactobacillus plantarum, Pediococcus lactis, Bacillus subtilis and Saccharomyces cerevisiae (8000r / m, the time is different according to the amount of fermentation broth, generally A batch of sludge can be produced in 40 minutes) to obtain active sludge of various strains. Put the active bacteria slime of each strain and the stabilizing protective agent in the mixing tank according to the mass ratio (0.5-3):(1-4), start the mixer, mix evenly for 25min, the stirring speed is 40r / m, and get the bacteria wet powder, and then the bacteria wet powder is frozen, and then freeze-dried to obtain probiotic powders of various strains.

[0134] The stabilized protective agent is prepared by uniformly mixing raw materials by weight percentage: 1-10% corn starch; 1-5% microcrystalline cellulose; 1-15% sodium alginate; 1-15% sodium carbo...

Embodiment 3

[0143] The preparation of embodiment 3 aquatic probiotics

[0144] The preparation of each bacterial agent of Rhodopseudomonas palustris, Enterococcus faecalis, Lactobacillus plantarum, Pediococcus lactis, Bacillus subtilis and Saccharomyces cerevisiae is the same as that in Example 2, and the aquatic probiotic agent is to combine Rhodopseudomonas palustris, feces Enterococcus, Lactobacillus plantarum, Pediococcus lactis, Bacillus subtilis, Saccharomyces cerevisiae inoculum and auxiliary materials are uniformly mixed according to the weight ratio of 2:1.2:2.1:0.8:3.2:1:2, and the activity is 50 billion CFU / g.

[0145] The percentage by weight of each component of described adjuvant is the same as in Example 2.

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