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Enzyme-linked immunosorbent assay detection kit and preparation method thereof

A detection kit, enzyme-linked immunosorbent assay technology, applied in measurement devices, instruments, scientific instruments, etc., can solve the problems of being infected with other diseases, and achieve the effect of wide application and accurate sensitivity

Inactive Publication Date: 2019-06-18
江苏伯纳德生物科技发展有限公司
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  • Abstract
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  • Claims
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Problems solved by technology

But at the same time of blood transfusion, it also bears the risk of being infected with other diseases

Method used

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  • Enzyme-linked immunosorbent assay detection kit and preparation method thereof

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Embodiment Construction

[0031] In order to have a further understanding and understanding of the structural features of the present invention and the achieved effects, the preferred embodiments and accompanying drawings are used for a detailed description, as follows:

[0032] Such as figure 1 As shown, an enzyme-linked immunoassay detection kit, including avidin-coated microwell plate, biotin-labeled HbsAb, biotin-labeled HCV gene recombinant antigen, biotin-labeled HIV gene recombinant antigen, horseradish Peroxidase-labeled HbsAb, horseradish peroxide-labeled mouse anti-human IgG, horseradish peroxidase-labeled HIV gene recombinant antigen, substrate A solution, substrate B solution, concentrated washing solution and stop solution.

[0033] In this embodiment, the microwell plate is a polystyrene microwell plate.

[0034] In this embodiment, the substrate A solution is a citrate buffer solution containing 2% hydrogen peroxide.

[0035] In this embodiment, the substrate B solution is a citrate bu...

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Abstract

The invention relates to an enzyme-linked immunosorbent assay detection kit and a preparation method thereof. The enzyme-linked immunosorbent assay kit comprises an avidin-coated microplate, a biotin-labeled HbsAb, a biotin-labeled HCV gene recombinant antigen, a biotin-labeled HIV gene recombination antigen, a horseradish peroxidase-labeled HbsAb, a horseradish peroxidase-labeled mouse anti-humanIgG, a horseradish peroxidase-labeled HIV gene recombinant antigen, a substrate solution A, a substrate solution B, a washing concentrate and a stop buffer. The enzyme-linked immunosorbent assay detection kit of the invention can be used to simultaneously detect an HBsAg, an HCV virus IgG antibody and an HIV virus antibody in a sample. The enzyme-linked immunosorbent assay detection kit for bloodsource screening has the advantages of quickness, simplicity, high accuracy, high sensitivity and wide application range. With the detection kit adopted, results can be judged and read with only 2 hours of whole operation time needed. The enzyme-linked immunosorbent assay detection kit is very suitable for blood source screening.

Description

technical field [0001] The invention relates to a blood source screening kit and a preparation method thereof, in particular to an enzyme-linked immunoassay detection kit and a preparation method thereof. Background technique [0002] People have to receive blood transfusions when they are bleeding heavily or if they have conditions such as severe anemia, leukemia, or myelofibrosis. However, blood transfusion also bears the risk of being infected with other diseases. Among the transfusion-transmitted diseases, HBV, HCV, and HIV are the main viruses that pose a serious threat to the safety of blood transfusion. The infection rate of HBV in our country is as high as 10.3%. Therefore, it seriously threatens the safety of blood transfusion in our country. The infection rate of HCV in the Chinese population is 1.0%-3.1%. Due to technical reasons, there is still a certain rate of missed detection of blood donors, resulting in 70% of transfusion-transmitted hepatitis being caused ...

Claims

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Application Information

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IPC IPC(8): G01N33/535G01N33/569
Inventor 孙伟
Owner 江苏伯纳德生物科技发展有限公司
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