House lizard/Paecilomyces cicadae bidirectional solid ferment and preparation method and application thereof

A two-way solid fermentation, Paecilomyces cicadae technology, applied in the direction of drug combination, pharmaceutical formula, plant raw materials, etc., can solve the problem of inability to prepare high-efficiency preparations, achieve the effect of improving anti-tumor function, expanding the way of medicine, and good application prospects

Active Publication Date: 2019-07-05
经皮永和医药科技(北京)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the clinical application of traditional Chinese medicine, geckos are mostly used as protozoa, original powder or ultrafine powder, which often has the defect that high-efficiency preparations cannot be prepared due to the large amount of preparation.

Method used

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  • House lizard/Paecilomyces cicadae bidirectional solid ferment and preparation method and application thereof
  • House lizard/Paecilomyces cicadae bidirectional solid ferment and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] The Gecko-Paecilomyces cicadae two-way solid fermentation product described in this embodiment is prepared according to the following steps:

[0034] (1) Gecko pretreatment: Weigh a number of dried gecko medicinal materials and put them into a 35-37°C constant-temperature blast drying oven for drying treatment for 48 hours, and pulverize them with a pulverizer, pass through a 60-mesh sieve, and the filtered product is the gecko powder used, and set aside ;

[0035] (2) Activation of Paecilomyces cicadae strains: pick an appropriate amount of Paecilomyces cicadae mycelium preserved with an inoculation loop, aseptically inoculate it on the sterilized PDA solid medium, and put it into a constant temperature and humidity culture at 28°C and 80% humidity Cultivate in the box for 5 days to obtain activated thalli; then, pick an appropriate amount of Paecilomyces cicadae mycelium from the PDA solid medium with an inoculation loop, insert it into the sterilized PDA liquid mediu...

Embodiment 2

[0040] The Gecko-Paecilomyces cicadae two-way solid fermentation product described in this embodiment is prepared according to the following steps:

[0041] (1) Gecko pretreatment: Weigh a number of dried gecko medicinal materials and put them into a 35-37°C constant-temperature blast drying oven for drying treatment for 48 hours, and pulverize them with a pulverizer, pass through a 60-mesh sieve, and the filtered product is the gecko powder used, and set aside ;

[0042] (2) Activation of Paecilomyces cicadae strains: pick an appropriate amount of Paecilomyces cicadae hyphae preserved with an inoculation loop, aseptically inoculate it on the sterilized PDA solid medium, and put it into a constant temperature and humidity culture at 25°C and 80% humidity Cultivate in the box for 7 days to obtain activated thalli; then, pick an appropriate amount of Paecilomyces cicadae mycelium from the PDA solid medium with an inoculation loop, insert it into the sterilized PDA liquid medium,...

Embodiment 3

[0047] The Gecko-Paecilomyces cicadae two-way solid fermentation product described in this embodiment is prepared according to the following steps:

[0048] (1) Gecko pretreatment: Weigh a number of dried gecko medicinal materials and put them into a 35-37°C constant-temperature blast drying oven for drying treatment for 48 hours, and pulverize them with a pulverizer, pass through a 60-mesh sieve, and the filtered product is the gecko powder used, and set aside ;

[0049] (2) Activation of Paecilomyces cicadae strains: pick an appropriate amount of Paecilomyces cicadae hyphae preserved with an inoculation loop, aseptically inoculate it on the sterilized PDA solid medium, and put it into a constant temperature and humidity culture at 30°C and 90% humidity Cultivate in the box for 4 days to obtain activated thalli; then, pick an appropriate amount of Paecilomyces cicadae mycelium from the PDA solid medium with an inoculation loop, insert it into the sterilized PDA liquid medium,...

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Abstract

The invention belongs to the technical field of traditional Chinese medicine preparations and particularly relates to a house lizard/Paecilomyces cicadae bidirectional solid ferment and further discloses a preparation method thereof and application of the house lizard/Paecilomyces cicadae bidirectional solid ferment in the preparation of liver cancer drugs. The house lizard/Paecilomyces cicadae bidirectional solid ferment is made by further fermenting a Paecilomyces cicadae liquid based on existing house lizard medicinal material; with unique properties and functional components of the house lizard and Paecilomyces cicadae, the antitumor function of the house lizard medicinal material is improved through deep interaction of the house lizard and Paecilomyces cicadae. The effective components of the house lizard/Paecilomyces cicadae bidirectional solid ferment are acquired by water extraction; the water extract can inhibit HepG2 liver cancer cells significantly and is suitable for the preparation of liver cancer drugs.

Description

technical field [0001] The invention belongs to the technical field of traditional Chinese medicine preparations, and specifically relates to a gecko-paecilomyces cicada bidirectional solid fermentation product, and further discloses a preparation method thereof, and its application in the preparation of drugs for treating liver cancer. Background technique [0002] Animal medicine is an important part of traditional Chinese medicine. There are 13 prescriptions in the classic of traditional Chinese medicine "Huangdi Neijing", of which 5 prescriptions are used as medicine with human or animal organs and tissues; , There are also a considerable number of records about animal medicinal materials. For example, the "Compendium of Materia Medica" compiled by Li Shizhen in the Ming Dynasty contained 1892 kinds of medicines, of which 444 were animal medicines, accounting for about 1 / 4 of the total. [0003] Gecko is a kind of animal medicine, and its main active ingredients are prot...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/068A61K35/583A61P35/00
CPCA61K35/583A61K36/068A61K2236/19A61K2236/331A61K2236/51A61P35/00A61K2300/00
Inventor 黄金昶史新元连增林杨芳
Owner 经皮永和医药科技(北京)有限公司
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