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Preparation method of multi-parameter high-throughput sers active microneedle and active microneedle
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A high-throughput, multi-parameter technology, applied in the field of biosensing, can solve the problem of not meeting the research needs of acupuncture effect mechanism
Active Publication Date: 2021-05-11
SOUTHEAST UNIV
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[0004] The research in the prior art can realize minimally invasive in vivo sampling and in vitro Raman detection of a single molecule (indicator), but it is still unable to meet the research on the mechanism of acupuncture effect through multi-target, multi-channel, multi-level and multi-level effects. need
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preparation example Construction
[0037] The invention provides a method for preparing a multi-parameter high-throughput SERS active microneedle, the preparation method comprising the following steps:
[0038] 1) Etching grooves on acupuncture needles by means of electrochemical etching, the specific implementation method is:
[0039] 1.1) Soak the acupuncture needles in an insulating polymer solution, and coat the needles of the acupuncture needles with a polymer insulating layer. The insulating polymer solution is polystyrene solution, polypropylene solution, polylactic acid solution or polymethacrylform Ester solution; Acupuncture needles are conventional acupuncture needles used clinically;
[0040] 1.2) Use a razor blade to scratch the polymer insulating layer on the body of the acupuncture needle at a distance of 2-4mm from the acupuncture needle tip, and use it as an etching site for etching the groove;
[0041] 1.3) In the current-time curve mode (0.1-1.5 volt potential), perform electrochemical etchi...
Embodiment 1
[0054] Embodiment 1 Groove etching on acupuncture needles
[0055] Acupuncture needles are soaked in insulating polymers such as 1% polystyrene toluene solution, etc., wrap an insulating layer on the body of the acupuncture needles, and then scratch the insulating layer with a blade as the etching site of the groove. In current-time curve mode (0.1-1.5 volt potential), perform electrochemical etching in 0.5 mol / L sulfuric acid for 5-60 seconds, according to figure 1 Shown etching device (this etching device belongs to prior art), can obtain such as figure 2 Represented grooves.
Embodiment 2
[0056] Embodiment 2 Groove functionalization
[0057] After the acupuncture needles etched with grooves are washed with ethanol, they are soaked in 1% 3-mercaptopropyltriethoxysilane and 3-mercaptopropyltrimethoxysilane ethanol solution for 2-48 hours, and then washed with ethanol Acupuncture needles with grooved thiol can be obtained.
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Abstract
The invention belongs to the technical field of biosensing, and relates to a method for preparing a multi-parameter high-throughput SERS active microneedle and an active microneedle. The preparation method includes the following steps: 1) using electrochemical etching to etch on acupuncture needles etching grooves; 2) constructing micro-nano gold structure arrays in the grooves obtained in step 1); 3) fabricating SERS microchips containing a variety of different responsive molecules based on the micro-nano gold structure arrays. The SERS active microneedle for multi-parameter detection prepared by the invention can be minimally invasive into the living body, realize minimally invasive in vivo sampling of various biological indicators, and in vitro Raman rapid detection, without sample pretreatment or consumption of additional reagents in the detection. It has the advantages of low cost, rapidity and simplicity, and is suitable for the research of new biomedical problems and the screening of large samples such as medicine and the environment.
Description
technical field [0001] The invention belongs to the technical field of biosensing, and relates to a preparation method of a SERS active microneedle and the active microneedle, in particular to a preparation method of a multi-parameter high-throughput SERS active microneedle and the active microneedle. Background technique [0002] In 2002, Ren et al reported an electrochemical detection method based on acupuncture needles to construct calcium ion sensing needles. Since 2015, Zhang GJ et al. have successively reported the electrochemical detection method of dopamine, 5-hydroxytryptamine and NO sensor needles based on acupuncture needles. The biggest advantage of the detection technology based on acupuncture needles is that it can be minimally invasive in and out of the living body to achieve in-vivo detection or in-vivo sampling and ex-vivo detection. If high-throughput detection can be realized, the electrochemical detection method will be an ideal in vivo detection method....
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