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Method for detecting CNV marker of SHE gene of Chaka sheep and application thereof

A gene and tea card technology, applied in the field of detection of CNV markers of Chaka sheep SHE gene, to achieve the effect of speeding up the establishment and breeding process, speeding up the breeding process, and easy operation

Active Publication Date: 2019-07-09
NORTHWEST A & F UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there is no relevant report on the functional research of SHE gene in sheep and other livestock

Method used

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  • Method for detecting CNV marker of SHE gene of Chaka sheep and application thereof
  • Method for detecting CNV marker of SHE gene of Chaka sheep and application thereof
  • Method for detecting CNV marker of SHE gene of Chaka sheep and application thereof

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Embodiment Construction

[0028] The present invention will be described in detail below in conjunction with the accompanying drawings and embodiments.

[0029] The present invention utilizes real-time fluorescent quantitative PCR to detect the copy number variation of Chaka sheep SHE gene and is used for molecular breeding, comprising the following steps:

[0030] (1) According to the sheep SHE gene sequence in the NCBI database, use Primer5.0 software to design primers;

[0031] (2) Use real-time fluorescent quantitative PCR (qPCR) technology to detect the copy number variation of candidate sites in the population;

[0032] (3) Using SPSS23.0 software to analyze the association between the type of copy number variation and the growth traits of sheep, and screen a CNV marker related to the growth traits of Chaka sheep; the CNV marker is located in the sheep SHE gene (GenBank AccessionNo.NC_019458.2) Within the 103174001-103176000 region of the reference sequence.

[0033] (4) Breeding dominant sheep...

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Abstract

The invention discloses a method for detecting a CNV marker of an SHE gene of Chaka sheep and application thereof. The method comprises the steps that based on a real-time fluorescent quantitative PCRtechnology, a copy number variation area of the SHE gene of the Chaka sheep is amplified by using the whole genome DNA of the Chaka sheep as a template, the amplified Chaka sheep ANKRD1 gene fragmentis used as an internal reference, and a 2*2-delta Ct method is used for calculating the copy number variation type of an individual. By correlating and analyzing the copy number variation with the growth trait, the method lays a foundation for establishing the association between the copy number variation of the Chaka sheep SHE gene and the body length trait, can be used for accelerating the breeding process of the length traits of the Chaka sheep, and is simple, rapid and easy to popularize and apply.

Description

technical field [0001] The invention belongs to the field of molecular genetics, and in particular relates to the detection of CNV markers of Chaka sheep SHE genes. Background technique [0002] Sheep are raised all over the world. They are docile and easy to domesticate. They can provide humans with products such as meat and fur. Body size traits and mutton quality are jointly regulated by multiple genes. With the deepening of genomic research, there is evidence that copy number variations (Copy Number Variations, CNVs) may affect the transcription and translation of related genes, thereby regulating gene networks, and then affecting individual phenotypic changes, so candidates related to body size traits are optimized DNA markers such as CNV markers can help speed up the sheep breeding process. [0003] Copy number variation generally refers to the structural variation of deletion, duplication and insertion greater than 50bp in the whole genome, which is caused by rearra...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6888C12Q1/6851
CPCC12Q1/6888C12Q1/6851C12Q2600/156C12Q2600/124C12Q2600/166C12Q2531/113C12Q2545/101C12Q2537/16
Inventor 陈宏蒋瑞程杰胡林勇黄永震蓝贤勇曹修凯
Owner NORTHWEST A & F UNIV