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Traditional Chinese medicine active site having HBV resisting effect and preparation method and application

An active site, traditional Chinese medicine technology, applied in the field of natural medicinal chemistry, can solve problems such as limiting clinical effects and threatening human health, and achieve significant anti-HBV effects.

Active Publication Date: 2019-07-19
JINAN UNIVERSITY
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  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

HBV is one of the main viruses causing global public health problems and seriously threatens human health. Although the widespread application of HBV vaccines and the promotion of infant and child immunization programs have played a positive role in the prevention of hepatitis B, the treatment of hepatitis B is still Difficult Issues of Public Concern
In the past ten years, the emergence of nucleoside analogs has made important progress in the treatment of hepatitis B. However, the emergence of drug-resistant virus variants caused by long-term use limits their clinical effects

Method used

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  • Traditional Chinese medicine active site having HBV resisting effect and preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Embodiment 1 has the preparation of the Chinese medicine active site of anti-HBV effect

[0024] The active part of traditional Chinese medicine with anti-HBV effect is obtained by extracting the active part of Chinese medicine with anti-HBV effect by using the traditional Chinese medicine Sophora Sophora as a raw material by supercritical carbon dioxide extraction;

[0025] The specific conditions of the supercritical carbon dioxide extraction method are as follows: ethyl acetate is used as an entrainer. The dosage of the entraining agent is 10%-15% of the mass of Sophora aphorae; the extraction pressure is 15 MPa, the extraction temperature is 35° C., the flow rate is 10 liters / hour, and the extraction time is 2.5 hours.

Embodiment 2

[0026] Embodiment 2 has the preparation of the Chinese medicine active site of anti-HBV effect

[0027] The active part of the traditional Chinese medicine with anti-HBV effect is extracted from the traditional Chinese medicine Sophora japonica by supercritical carbon dioxide extraction method;

[0028] The specific conditions of the supercritical carbon dioxide extraction method are as follows: ethyl acetate is used as an entrainer. The amount of entraining agent is 10% to 15% of the mass of Sophora aphorae; the extraction pressure is 15 MPa, the extraction temperature is 35° C., the flow rate is 10 liters / hour, and the extraction time is 2.5 hours;

[0029] Further, the Sophora Sophorae extract obtained after supercritical carbon dioxide extraction is subjected to ODS column chromatography;

[0030] The specific method of the ODS column chromatography is as follows: put the Sophora Sophora extract on the ODS column, first use 45% methanol with a volume fraction of 3 times t...

Embodiment 3

[0037] Embodiment 3 Chinese medicine active site anti-HBV viral activity test

[0038] Experimental group:

[0039] Respectively test the Chinese medicine active site (experimental group 1) with anti-HBV effect prepared in Example 1, the Chinese medicine active site with anti-HBV effect prepared in Example 2 (experimental group 2), the Chinese medicine activity prepared in Comparative Example 1 site (experimental group 3) and oxymatrine (control group) anti-HBV virus activity.

[0040] experimental method:

[0041] Dilute HepG2.2.15 cells with MEM medium to 4×10 4 Inoculate / well in a 24-well plate at 37°C, 5% CO 2Cultivate for 24 hours in a constant temperature incubator. When the cells reached 90% confluence, the supernatant was discarded, and then drug dilutions (1.0 mL) of each experimental group were added at serial concentrations (concentration range: 0.1-200 μg / mL). The culture was continued under the same conditions, and the fresh drug solution of the original conc...

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Abstract

The invention relates to the technical field of natural medicine chemistry, and specifically discloses a traditional Chinese medicine active site having the HBV resisting effect and a preparation method and application. According to the method, sophora alopecuroides seeds as a traditional Chinese medicine are used as a raw material, and the traditional Chinese medicine active site is obtained through extraction by a supercritical carbon dioxide extraction method, wherein specific condition of the supercritical carbon dioxide extraction method includes that the extraction pressure is 10-30 megapascal, the extraction temperature is 30-40 DEG C, the flow rate is 5-15 litre / hour, and the extraction time is 2-3 hours. The traditional Chinese medicine active site prepared by the method has remarkable HBV resisting effect, and the HBV resisting effect is equivalent to and even higher than the HBV resisting effect of oxymatrine as a main component in existing hepatitis B treating medicines including kurarinone capsules and kurarinone tablets.

Description

technical field [0001] The invention relates to the technical field of natural medicinal chemistry, in particular to an active part of a traditional Chinese medicine with an anti-HBV effect, a preparation method and an application. Background technique [0002] Hepatitis B Virus (HBV) infection can cause acute and chronic hepatitis B. It is one of the most important causes of liver cirrhosis, liver cancer and other serious liver diseases. It has long been a serious worldwide medical and health issue. problem. According to WHO statistics, about 2 billion people in the world have been infected with HBV, of which 350-400 million become chronic carriers, and 15%-40% of chronic HBV-infected patients can develop liver cirrhosis or hepatocellular carcinoma (Hepatocellularcarcinoma, HCC). ), about 1 million people worldwide die from HBV-related diseases every year. HBV is one of the main viruses causing global public health problems and seriously threatens human health. Although t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/489A61P31/20A61P1/16
CPCA61K36/489A61K2236/37A61P1/16A61P31/20
Inventor 王国才李药兰张玉波张晓丽杨丽陈能花吴忠南
Owner JINAN UNIVERSITY
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