A kind of active part of traditional Chinese medicine with anti-HBV effect, preparation method and application
An active part and traditional Chinese medicine technology, applied in the field of natural medicinal chemistry, can solve problems such as limiting clinical effects and threatening human health, and achieve significant anti-HBV effects
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Embodiment 1
[0023] Embodiment 1 has the preparation of the Chinese medicine active site of anti-HBV effect
[0024] The active part of traditional Chinese medicine with anti-HBV effect is obtained by extracting the active part of Chinese medicine with anti-HBV effect by using the traditional Chinese medicine Sophora Sophora as a raw material by supercritical carbon dioxide extraction;
[0025] The specific conditions of the supercritical carbon dioxide extraction method are as follows: ethyl acetate is used as an entrainer. The dosage of the entraining agent is 10%-15% of the mass of Sophora aphorae; the extraction pressure is 15 MPa, the extraction temperature is 35° C., the flow rate is 10 liters / hour, and the extraction time is 2.5 hours.
Embodiment 2
[0026] Embodiment 2 has the preparation of the Chinese medicine active site of anti-HBV effect
[0027] The active part of the traditional Chinese medicine with anti-HBV effect is extracted from the traditional Chinese medicine Sophora japonica by supercritical carbon dioxide extraction method;
[0028] The specific conditions of the supercritical carbon dioxide extraction method are as follows: ethyl acetate is used as an entrainer. The amount of entraining agent is 10% to 15% of the mass of Sophora aphorae; the extraction pressure is 15 MPa, the extraction temperature is 35° C., the flow rate is 10 liters / hour, and the extraction time is 2.5 hours;
[0029] Further, the Sophora Sophorae extract obtained after supercritical carbon dioxide extraction is subjected to ODS column chromatography;
[0030] The specific method of the ODS column chromatography is as follows: put the Sophora Sophora extract on the ODS column, first use 45% methanol with a volume fraction of 3 times t...
Embodiment 3
[0037] Embodiment 3 Chinese medicine active site anti-HBV viral activity test
[0038] Experimental group:
[0039] Respectively test the Chinese medicine active site (experimental group 1) with anti-HBV effect prepared in Example 1, the Chinese medicine active site with anti-HBV effect prepared in Example 2 (experimental group 2), the Chinese medicine activity prepared in Comparative Example 1 site (experimental group 3) and oxymatrine (control group) anti-HBV virus activity.
[0040] experimental method:
[0041] Dilute HepG2.2.15 cells with MEM medium to 4×10 4 Inoculate / well in a 24-well plate at 37°C, 5% CO 2In a constant temperature incubator, cultivate for 24 hours. When the cells reached 90% confluence, the supernatant was discarded, and then drug dilutions (1.0 mL) of each experimental group were added at serial concentrations (concentration range: 0.1-200 μg / mL). The culture was continued under the same conditions, and the fresh drug solution of the original con...
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