Traditional Chinese medicine composition and preparation method and application thereof
A composition and technology of traditional Chinese medicine, applied in the field of traditional Chinese medicine composition and its preparation, can solve the problems of not being able to stop the drug casually, easy to relapse, ALT increase, etc., and achieve good anti-hepatitis B virus application prospects and significant anti-hepatitis B virus effect
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Embodiment 1
[0036] Embodiment 1: Chinese medicine composition Soxhlet extraction method of the present invention
[0037] 1. Prescription
[0038] 120 parts of gardenia, 180 parts of capillary, 120 parts of coptis, and 20 parts of evodia.
[0039] 2. Preparation method
[0040]a. Prepare 90% ethanol: Take 900mL of absolute ethanol, add 100mL of deionized water, mix up and down three times.
[0041] b. Put 10 g of medicinal materials wrapped in filter paper into a Soxhlet extractor, and extract with 100-200 mL of 90% ethanol (material ratio 1:10-20). After the extraction, the sample was dried in a drying oven at 45°C and evaporated to 2 mL. Then transfer to a 10mL brown vial, store in a -80°C refrigerator, and freeze for at least 6 hours.
[0042] c. Take out the brown vial containing the sample from the -80°C refrigerator, use a lyophilizer to freeze-dry it into a powder, and store it for future use.
Embodiment 2
[0043] Embodiment 2: ultrasonic preparation method of Chinese medicine composition of the present invention
[0044] 1. The prescription is the same as that in Example 1 (120 parts of Gardenia, 180 parts of capillary, 120 parts of Coptidis Rhizome, 20 parts of Evodia rutaecarpa), and it is pulverized into powder by using a pulverizer.
[0045] 2. According to the material ratio of 1:10-20, add 90% ethanol solution to soak for 1-2h, ultrasonically extract for 10-30min, let stand fully, and concentrate the supernatant at 70°C.
[0046] 3. Take the concentrated solution, freeze it at -80°C for more than 6 hours, and dry it in a low-temperature vacuum to obtain a dry powder.
Embodiment 3
[0047] Embodiment 3: Chinese medicine composition anti-hepatitis B virus experiment of the present invention
[0048] 1. Materials and methods
[0049] (1) The traditional Chinese medicine composition of the present invention: take 50 mg of the lyophilized powder of the alcohol extract prepared according to the method of Example 1 of the present invention, add 1 mL of DMSO, and configure it into a 50 mg / mL concentrated solution.
[0050] (2) Configuration of the experimental working solution of the traditional Chinese medicine composition of the present invention: according to the requirements of the experiment, the concentrated solution was diluted with a culture medium to a working solution of 100 μg / mL.
[0051] (3) HepG2.2.15 cell plating: For the liver cytotoxicity test, HepG2.2.15 cells were used. Take the culture flasks of two kinds of cells from the incubator, add 1mL of trypsin to the culture flasks, shake gently so that the entire cell surface is covered with trypsi...
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