Traditional Chinese medicine composition and preparation method and application thereof

A composition and technology of traditional Chinese medicine, applied in the field of traditional Chinese medicine composition and its preparation, can solve the problems of not being able to stop the drug casually, easy to relapse, ALT increase, etc., and achieve good anti-hepatitis B virus application prospects and significant anti-hepatitis B virus effect

Active Publication Date: 2021-02-26
CAPITALBIO CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Interferon, nucleoside analogs and other liver disease antiviral western medicines mainly inhibit the replication of DNA. The disadvantage is that the medicine cannot be stopped casually. Long-term use will cause side effects such as flu-like, anorexia, nausea, diarrhea, depression, angina pectoris, etc.
In addition, although lamivudine and other nucleoside antiviral drugs can quickly inhibit the virus, after stopping the drug, the virus will replicate quickly, ALT will increase, and some individuals will develop severe liver disease and make the disease worse
These drugs have rapid curative effect, but are prone to relapse and have certain side effects

Method used

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  • Traditional Chinese medicine composition and preparation method and application thereof
  • Traditional Chinese medicine composition and preparation method and application thereof
  • Traditional Chinese medicine composition and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Embodiment 1: Chinese medicine composition Soxhlet extraction method of the present invention

[0037] 1. Prescription

[0038] 120 parts of gardenia, 180 parts of capillary, 120 parts of coptis, and 20 parts of evodia.

[0039] 2. Preparation method

[0040]a. Prepare 90% ethanol: Take 900mL of absolute ethanol, add 100mL of deionized water, mix up and down three times.

[0041] b. Put 10 g of medicinal materials wrapped in filter paper into a Soxhlet extractor, and extract with 100-200 mL of 90% ethanol (material ratio 1:10-20). After the extraction, the sample was dried in a drying oven at 45°C and evaporated to 2 mL. Then transfer to a 10mL brown vial, store in a -80°C refrigerator, and freeze for at least 6 hours.

[0042] c. Take out the brown vial containing the sample from the -80°C refrigerator, use a lyophilizer to freeze-dry it into a powder, and store it for future use.

Embodiment 2

[0043] Embodiment 2: ultrasonic preparation method of Chinese medicine composition of the present invention

[0044] 1. The prescription is the same as that in Example 1 (120 parts of Gardenia, 180 parts of capillary, 120 parts of Coptidis Rhizome, 20 parts of Evodia rutaecarpa), and it is pulverized into powder by using a pulverizer.

[0045] 2. According to the material ratio of 1:10-20, add 90% ethanol solution to soak for 1-2h, ultrasonically extract for 10-30min, let stand fully, and concentrate the supernatant at 70°C.

[0046] 3. Take the concentrated solution, freeze it at -80°C for more than 6 hours, and dry it in a low-temperature vacuum to obtain a dry powder.

Embodiment 3

[0047] Embodiment 3: Chinese medicine composition anti-hepatitis B virus experiment of the present invention

[0048] 1. Materials and methods

[0049] (1) The traditional Chinese medicine composition of the present invention: take 50 mg of the lyophilized powder of the alcohol extract prepared according to the method of Example 1 of the present invention, add 1 mL of DMSO, and configure it into a 50 mg / mL concentrated solution.

[0050] (2) Configuration of the experimental working solution of the traditional Chinese medicine composition of the present invention: according to the requirements of the experiment, the concentrated solution was diluted with a culture medium to a working solution of 100 μg / mL.

[0051] (3) HepG2.2.15 cell plating: For the liver cytotoxicity test, HepG2.2.15 cells were used. Take the culture flasks of two kinds of cells from the incubator, add 1mL of trypsin to the culture flasks, shake gently so that the entire cell surface is covered with trypsi...

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Abstract

The invention relates to the technical field of traditional Chinese medicines and discloses a traditional Chinese medicine composition as well as a preparation method and application thereof. The traditional Chinese medicine composition is an extract of herba artemisiae scopariae, fructus gardeniae, coptidis rhizoma and fructus evodiae. The traditional Chinese medicine composition with an obviousanti-hepatitis B virus effect is prepared from the herba artemisiae scopariae, the fructus gardeniae, the coptidis rhizoma and the fructus evodiae by compounding, and has an obvious inhibition effecton HBsAg and HBeAg antigen secretion, and meanwhile, the fructus gardeniae and the herba artemisiae scopariae are used for regulating the toxicity of the coptidis rhizoma and the fructus evodiae on hepatocytes, so that the whole prescription has no obvious inhibition effect on the activity of the hepatocytes; and meanwhile, compared with each single medicine, the traditional Chinese medicine composition has a remarkable anti-hepatitis B virus effect. In addition, the n-butyl alcohol extraction active part of the traditional Chinese medicine composition also has no obvious HepG2.2.15 hepatotoxicity, and has an obvious inhibition effect on HBsAg and HBeAg antigen secretion.

Description

technical field [0001] The invention relates to the technical field of traditional Chinese medicine preparations, in particular to a traditional Chinese medicine composition and its preparation method and application. Background technique [0002] The liver is the largest solid organ in the abdominal cavity and is responsible for important physiological functions of the human body. Its main function is to regulate the material metabolism and energy metabolism of the whole body, and it is also an important immune organ. Stimulation of the liver or infection such as infectious diseases, immunodeficiency diseases, and chemical poisons can cause liver damage. In my country, viral hepatitis, especially chronic hepatitis B, is the most common cause of liver damage and eventually cirrhosis. Anti-HBV treatment is the key to prevent the further development of liver damage. [0003] Antiviral therapy is the fundamental treatment for chronic hepatitis B. For hepatitis B patients who...

Claims

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Application Information

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IPC IPC(8): A61K36/754A61P31/20A61P1/16
CPCA61K36/282A61K36/744A61K36/718A61K36/754A61P31/20A61P1/16A61K2236/333A61K2236/55A61K2300/00Y02A50/30
Inventor 郭弘妍王辉郝艳同邢韶芳程京
Owner CAPITALBIO CORP
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