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Application of herba chloranthi serrati extract in preparation of drugs for resisting hepatitis B virus and/or preventing and treating hepatitis B

A hepatitis B virus, hepatitis B technology, applied in the direction of antiviral agents, anti-inflammatory agents, drug combinations, etc., can solve the problems of anti-HBV, anti-inflammatory and liver protection, etc., and achieve good anti-HBV application. Prospect, good anti-liver injury, good effect of regulating function of liver inflammation

Pending Publication Date: 2021-04-09
CAPITALBIO CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there is no report on anti-hepatitis B virus, anti-inflammatory and liver protection

Method used

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  • Application of herba chloranthi serrati extract in preparation of drugs for resisting hepatitis B virus and/or preventing and treating hepatitis B
  • Application of herba chloranthi serrati extract in preparation of drugs for resisting hepatitis B virus and/or preventing and treating hepatitis B
  • Application of herba chloranthi serrati extract in preparation of drugs for resisting hepatitis B virus and/or preventing and treating hepatitis B

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Experimental program
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preparation example Construction

[0035] The preparation method of the Siguawa extract is as follows: the Siguawa adopts the Soxhlet extraction method, uses 90% ethanol as a solvent, heats and refluxes for extraction, filters and concentrates, freezes and dries to obtain the Siguawa alcohol extract.

[0036] The four tile alcohol extracts of the present invention have the following effects:

[0037] (1) It has obvious anti-hepatitis B virus effect. In the present invention, the Soxhlet extraction method is used to use ethanol with a concentration of 90% as a solvent for the four tiles, heat and reflux extraction, filter and concentrate, and freeze-dry to obtain the four tiles extract. Based on the HepG2.2.15 cell hepatitis B virus model, the anti-hepatitis B drug was screened from the extract of Siguawa, and the CCK8 cell proliferation test showed that the extract of Siguawa had no cytotoxic effect on HepG2.2.15, and it had obvious effects on the secretion of HBsAg and HBeAg antigens inhibitory effect.

[0038...

Embodiment 1

[0042] Embodiment 1: the Soxhlet preparation method of four tiles ethanol extract

[0043] 1. Take 10g of four tiles and use a pulverizer to crush them into powder.

[0044] 2. Wrap the powdered four tiles in filter paper and place them in a Soxhlet extractor, and add 150mL of 90% ethanol.

[0045] 3. Use a Soxhlet extractor to conduct heating and reflux extraction for 3 hours.

[0046] 4. After the extract is filtered, it is concentrated at 45°C to obtain an extract.

[0047] 5. Obtain the ethanol extract of the four tiles by freeze-drying for 24 hours.

[0048] Collect the freeze-dried four tile ethanol extracts and store them in a -80°C refrigerator for later use

Embodiment 2

[0049] Embodiment 2: Ultrasonic preparation method of Sikuwa extract

[0050] 1. Take 10g of four tiles and use a pulverizer to crush them into powder.

[0051] 2. According to the material ratio of 1:12, add 90% ethanol solution to soak for 1-2h, ultrasonically extract for 10-30min, let stand fully, and concentrate the supernatant at 70°C.

[0052] 3. Take the concentrated solution, freeze it at -80°C for more than 6 hours, and dry it in a low-temperature vacuum to obtain a dry powder.

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Abstract

The invention relates to the technical field of natural drugs, and particularly relates to a preparation method and application of a herba chloranthi serrati extract. The invention provides the application of the herba chloranthi serrati extract. A CCK8 cell proliferation detection experiment shows that the herba chloranthi serrati extract has no cytotoxicity effect on HepG2.2.15, has an obvious inhibiting effect on HBsAg and HBeAg antigen secretion, and shows a better anti-hepatitis B virus application prospect. The content of AST in HepG2.2.15 cell supernatant can be reduced, and a better anti-liver injury effect is shown. Huvec cell model transcriptome data analysis shows that the gene expression level of inflammatory pathways such as a TNF signal pathway and an IL-17 signal pathway can be effectively regulated, and a better liver inflammation regulating function is achieved.

Description

technical field [0001] The present invention relates to the technical field of natural medicines, in particular to the application of Sicuwa extract in the preparation of medicines for resisting hepatitis B virus and / or preventing and treating hepatitis B. Background technique [0002] The liver is the largest solid organ in the abdominal cavity and is responsible for important physiological functions of the human body. Its main function is to regulate the material metabolism and energy metabolism of the whole body, and it is also an important immune organ. Stimulation of the liver or infection such as infectious diseases, immunodeficiency diseases, and chemical poisons can cause liver damage. When the liver is damaged, inflammation will appear in the liver, such as liver cell necrosis, degeneration, swelling, different degrees of inflammatory infiltration, fibrous hyperplasia, etc., which will affect liver function. Persistent inflammation can disrupt tissue integrity, ch...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/185A61P31/20A61P1/16A61P29/00
CPCA61K36/185A61P31/20A61P1/16A61P29/00A61K2236/333A61K2236/39A61K2236/53A61K2236/51
Inventor 郭弘妍王辉郝艳同刘京京邢婉丽程京
Owner CAPITALBIO CORP
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