Method of constructing rice molecular marker map based on competitive allelic PCR and application in rice molecular breeding thereof

A molecular marker and competitive technology, applied in the agricultural field, can solve the problems of unclear SNP information, unclear relationship between SNP markers and traits, and difficulty in SNP detection, and achieve the effects of easy automation, simple detection method and low cost.

Pending Publication Date: 2019-07-19
SOUTH CHINA AGRI UNIV
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Problems solved by technology

[0008] Aiming at the problem that the current rice SNP is out of touch with breeding practice, the present invention combines the integrated SNP map with competitive allelic PCR, w

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  • Method of constructing rice molecular marker map based on competitive allelic PCR and application in rice molecular breeding thereof
  • Method of constructing rice molecular marker map based on competitive allelic PCR and application in rice molecular breeding thereof
  • Method of constructing rice molecular marker map based on competitive allelic PCR and application in rice molecular breeding thereof

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Embodiment Construction

[0027] The present invention will be further described below in conjunction with the accompanying drawings and specific embodiments. The test methods used in the examples are conventional methods unless otherwise specified; the materials and reagents used are commercially available reagents and materials unless otherwise specified.

[0028] 1. Collection of 530 rice materials from different sources at home and abroad

[0029] A total of 530 germplasm materials were obtained from a wide range of sources, and the sources of different materials are shown in Table 1. Among them, two types of imported germplasm (experimental IDs are KY and KIR) are from the International Rice Research Institute, and the rest of the materials are provided by South China Agricultural University.

[0030]530 rice materials collected in Table 1

[0031]

[0032]

[0033] 2. Genome resequencing analysis of 530 rice materials

[0034] The DNA of 530 rice materials was extracted, and the shotgun ...

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Abstract

The invention provides a method of constructing a rice molecular marker map based on competitive allelic PCR and an application in rice molecular breeding thereof, and belongs to the technical field of agriculture. The method aims to search for SNP sites by whole gene recombination sequencing of rice and mining SNP data of recombination sequencing; 565 SNP sites needed for breeding are obtained through combining existing SNP databases based on the SNP sites; the selected 565 SNP sites are utilized to perform molecular marking, and the rice molecular marker map is constructed. Physical locationof SNP markers of the rice molecular marker map constructed by the method is clear, detection method is simple. The rice molecular marker map can be directly used for partial molecular identificationand genetic background analysis of important agronomic traits, and has important application value to improve the efficiency of the rice molecular breeding.

Description

technical field [0001] The invention relates to a method for constructing a rice molecular marker map based on competitive allele PCR and its application for breeding, belonging to the field of agricultural technology. Background technique [0002] DNA molecular markers are polymorphic nucleotide sequences used to identify differences between two individuals or breeds and follow a simple Mendelian inheritance pattern. High polymorphism, co-dominant inheritance, frequent occurrence in the genome, selection neutral behavior (that is, no pleiotropy), simple operation and easy and rapid determination, good reproducibility and easy data exchange in the laboratory are ideal DNA markers Characteristics. Over the past few decades, the use of molecular markers to reveal polymorphisms at the DNA level has played an increasingly important role in plant biotechnology and its genetics research. [0003] Since the birth of single nucleotide polymorphism (SNP) in 1994, it has been quickl...

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Application Information

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IPC IPC(8): C12Q1/6895
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 郭涛陈志强王慧刘永柱王加峰肖武名黄明杨瑰丽黄翠红周丹华陈淳孙凯
Owner SOUTH CHINA AGRI UNIV
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