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Nasopharygeal carcinoma resisting active composition and chemotherapy drug as well as application of nasopharygeal carcinoma resisting active composition

A chemotherapy drug and technology for nasopharyngeal carcinoma, applied in drug combinations, antineoplastic drugs, inorganic active ingredients, etc., can solve the problems of low survival rate, large weight change, and large toxic and side effects of the patient, so as to improve the effect of chemotherapy and enhance the Effect, the effect of reducing IC50 value

Pending Publication Date: 2019-07-23
TCM INTEGRATED HOSPITAL OF SOUTHERN MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] In order to solve the shortcomings of the existing nasopharyngeal carcinoma, such as poor effect, low survival rate, large body weight change, and large toxic and side effects, the present invention provides an anti-nasopharyngeal carcinoma active composition, which aims to improve the anti-nasopharyngeal carcinoma. effects, reduce toxic and side effects, prolong the life of organisms, and improve the quality of life

Method used

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  • Nasopharygeal carcinoma resisting active composition and chemotherapy drug as well as application of nasopharygeal carcinoma resisting active composition
  • Nasopharygeal carcinoma resisting active composition and chemotherapy drug as well as application of nasopharygeal carcinoma resisting active composition
  • Nasopharygeal carcinoma resisting active composition and chemotherapy drug as well as application of nasopharygeal carcinoma resisting active composition

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] MTT method to detect the effect of DDP combined with the compound of formula 1 on the IC50 value of nasopharyngeal carcinoma cells

[0038] Human nasopharyngeal carcinoma cells HONE1-EBV grown in logarithmic phase + and 5-8F, after trypsinization for about five minutes, lightly suspend into a single cell suspension and count, adjust the number of cells to 5×10 in 100ul per well 3 After 24 hours of conventional culture, after the cells adhere to the wall and grow well, suck off the old culture medium (RPMI-1640), and add different concentrations of the compound of formula 1 and DDP liquid prepared from the culture medium, 0.01, 0.25, 0.5, Set five duplicate wells for each concentration of 0.75, 1μM, continue to cultivate for 48 hours, add 20μl of MTT, incubate in a 37° incubator for 4 hours, suck off the old medium containing MTT, add 150μl of DMSO to dissolve, and put it in a MultiskanSky microplate reader The absorbance value (OD value) of each well was detected with ...

Embodiment 2

[0042] Drug experiment in nude mice

[0043] will contain 5 x 10 6 HONE1-EBV overexpressing EBV-miR-BART22 + and 5-8F cells were injected into nude mice by intraperitoneal injection, and then the nude mice were randomly divided into four groups, 10 in each group. 10 days after injection, the first group received DDP treatment (4mg / kg), the second group received formula 1 compound treatment (4mg / kg), the third group DDP joint formula 1 compound treatment (total 4mg / kg; wherein, DDP, The ratio of the compound of formula 1 was 1:1); each group was administered by intraperitoneal injection every 5 days. The nude mice were weighed regularly, the survival of the nude mice was observed every day, and the time of death was recorded in time.

[0044] 1. Observation of kidney tissue morphology

[0045] 2. Result statistics

[0046] Survival analysis Grafpad was used to draw survival curves.

[0047] The results show:

[0048] The present invention adopts formula 1 compound and DD...

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Abstract

The invention belongs to the field of nasopharygeal carcinoma drugs and particularly relates to a nasopharygeal carcinoma resisting active composition comprising cisplatin and a compound with a structural formula in formula 1. The invention also discloses an application of the nasopharygeal carcinoma resisting active composition and a chemotherapy drug prepared from the nasopharygeal carcinoma resisting active composition. By combining the compound in the formula 1 with the cisplatin, the composition has unexpected effects in multiple aspects. For example, the composition can synergistically improve sensitivity to nasopharygeal carcinoma cells, reduce IC50 and significantly enhance the nasopharygeal carcinoma resisting effect and can also synergistically prolong the survival time of sick bodies, improve the weight state and reduce toxic and side effects, particularly renal toxicity.

Description

[0001] Technical field: [0002] The invention belongs to the field of medicines, in particular to a medicine for nasopharyngeal carcinoma. [0003] Background technique: [0004] Tumor is one of the main causes of human death. Nasopharyngeal carcinoma is a malignant tumor in South China and Southeast Asian countries. Its main characteristics are highly regional, racial, prevalent, and prone to distant metastasis in the early stage. [0005] The current main treatment methods for nasopharyngeal carcinoma are surgical resection, biological immunotherapy, radiotherapy combined with chemotherapy drugs, etc., among which platinum drugs are the first choice for conventional chemotherapy drugs for nasopharyngeal carcinoma. [0006] Cisplatin, one of the platinum drugs, is the first-line drug for clinical chemotherapy of nasopharyngeal carcinoma, but it often induces drug resistance in nasopharyngeal carcinoma during chemotherapy, which seriously affects the therapeutic effect and pro...

Claims

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Application Information

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IPC IPC(8): A61K33/243A61P35/00A61K31/585
CPCA61K31/585A61K33/24A61P35/00A61K2300/00
Inventor 方唯意刘祎祎刘真
Owner TCM INTEGRATED HOSPITAL OF SOUTHERN MEDICAL UNIV