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A detection kit for chronic obstructive pulmonary disease
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A chronic obstructive pulmonary and disease detection technology, applied in biological testing, measuring devices, material inspection products, etc., can solve problems such as time-consuming, expensive, and indistinguishable, and achieve good market prospects
Active Publication Date: 2022-03-04
WEST CHINA HOSPITAL SICHUAN UNIV
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[0003] At present, the diagnosis of COPD mainly relies on pulmonary function tests, chest X-ray examinations, chest CT examinations, etc. However, pulmonary function tests (spirometry) are time-consuming and expensive procedures that can only be performed by specialist pulmonary physicians
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[0030] 80 healthy controls, 126 COPD patients and 146 AECOPD patients were included. All subjects were informed and agreed before the experiment.
[0031] 1.2 Pulmonary function test
[0032] Check steps:
[0033] (1) Ask about the medical history, and explain the purpose, process and precautions to the patient before the operation;
[0034] (2) Enter the basic information of the patient;
[0035] (3) Clamp the patient's nose with the turbinate, and instruct the patient to keep breathing through the mouth;
[0036] (4) Tighten the disposable mouthpiece as much as possible to ensure no air leakage during the test;
[0037] (5) According to the inspection items, guide the patient to cooperate with immediate exhalation and inhalation actions through passwords;
[0038] (6) Collect data, analyze and print reports;
[0039] The meaning of t...
Embodiment 2
[0063] Embodiment 2 Kit of the present invention
[0064] 1. the composition of kit of the present invention
[0065]
[0066] 2. How to use the kit
[0067] (1) Add 50 μL of standard or sample to each well of a 96-well plate, seal the film, and incubate with shaking at room temperature for 2 hours;
[0068] (3) Wash the plate 3 times with 100 μL plate washing solution;
[0069] (4) Add 50 μL of biotin-labeled secondary antibody to each well, seal the membrane and incubate at room temperature for 30 minutes with shaking;
[0070] (5) Wash the plate 3 times with 100 μL plate washing solution;
[0071] (6) Add 50 μL of chromogenic substrate to each well, shake and mix at room temperature for 2 minutes;
[0073] In summary, the present invention can realize effective detection of COPD by detecting Thrombospondin-2 protein in blood.
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Abstract
The invention relates to the field of in vitro diagnosis of lung diseases, in particular to a detection kit for chronic obstructive pulmonary disease. The kit of the present invention realizes the diagnosis of chronic obstructive pulmonary disease by detecting the content of Thrombospondin-2 in blood. The kit is simple and effective, and has good application prospects.
Description
technical field [0001] The invention relates to the field of in vitro diagnosis of lung diseases, in particular to a detection kit for chronic obstructive pulmonary disease. Background technique [0002] Chronic obstructive pulmonary disease (COPD) is a chronic bronchitis and / or emphysema characterized by airflow obstruction, which can further develop into a common chronic disease of pulmonary heart disease and respiratory failure. It is related to the abnormal inflammatory response of harmful gases and particles, and the disability rate and mortality rate are very high. The global incidence rate of over 40 years old has reached as high as 9% to 10%. [0003] At present, the diagnosis of COPD mainly relies on pulmonary function tests, chest X-ray examinations, chest CT examinations, etc. However, pulmonary function tests (spirometry) are time-consuming and expensive procedures that can only be performed by specialist pulmonary physicians. However, the above methods cannot b...
Claims
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Application Information
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