Cultivation method of edible fungi rich in superoxide dismutase (SOD)
A cultivation method and technology of edible fungus, applied in the field of agriculture, can solve problems such as the research of unseen SOD content
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Embodiment 1
[0018] (1) Inoculation stage: mix the cultivation materials with the aforementioned proportions, put them into a polyethylene folding bag and tie them up, put them in a sterilization pot for high temperature and full sterilization, after sterilization, let the temperature in the pot naturally drop to 60℃ Take out the rod while it is hot and move it into the inoculation room. When the temperature of the rod drops below 30℃, inoculate black fungus and spray 15-25 billion CFU / mL Bacillus cereus (CGMCCNO.0175) water solution to the surface to wet;
[0019] (2) Mycelium culture stage: After inoculation, the sticks are placed in a dark, dry and ventilated environment for mycelial culture. The environment temperature of the site is about 25℃, the air humidity is below 70%, and the mycelium is cultured for 2 months. , During the period according to the ambient temperature and the growth of mycelium, turn over, pile up and ventilate;
Embodiment 2
[0025] (1) Inoculation stage: mix the cultivation materials with the aforementioned proportions, put them into a polyethylene folding bag and tie them up, put them in a sterilization pot for high temperature and full sterilization, after sterilization, let the temperature in the pot naturally drop to 60℃ Take out the rod while it is hot and move it into the inoculation room. When the temperature of the rod drops below 30℃, inoculate black fungus and spray 15-25 billion CFU / mL Bacillus cereus (CGMCCNO.0760) water solution to the surface to wet;
[0026] (2) Mycelium culture stage: After inoculation, the sticks are placed in a dark, dry and ventilated environment for mycelial culture. The environment temperature of the site is about 25℃, the air humidity is below 70%, and the mycelium is cultured for 2 months. , During the period according to the ambient temperature and the growth of mycelium, turn over, pile up and ventilate;
Embodiment 3
[0032] (1) Inoculation stage: mix the cultivation materials with the aforementioned proportions, put them into a polyethylene folding bag and tie them up, put them in a sterilization pot for high temperature and full sterilization, after sterilization, let the temperature in the pot naturally drop to 60℃ Take out the rod while it is hot and move it into the inoculation room. When the temperature of the rod falls below 30℃, inoculate black fungus and spray 15-25 billion CFU / mL Bacillus cereus (CGMCCNO.0761) water solution to the surface to wet;
[0033] (2) Mycelium culture stage: After inoculation, the sticks are placed in a dark, dry and ventilated environment for mycelial culture. The environment temperature of the site is about 25℃, the air humidity is below 70%, and the mycelium is cultured for 2 months. , During the period according to the ambient temperature and the growth of mycelium, turn over, pile up and ventilate;
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