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Application of long-chain non-coding RNA lncLCIR-1 as lung cancer molecular marker

A technology of long-chain non-coding and molecular markers, which can be used in medical preparations containing active ingredients, measurement/testing of microorganisms, drug combinations, etc., can solve the problems of little research on the role of lncRNA, and achieve the ability to inhibit invasion and metastasis, Good stability and anti-proliferation effect

Active Publication Date: 2019-07-30
SHANDONG RES INST OF TUMOUR PREVENTION TREATMENT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It has been reported that some lncRNAs can affect the radiosensitivity of tumor cells, but most of them are concentrated in reproductive system and digestive system tumors, and the role of lncRNAs in lung cancer radiotherapy is rarely studied

Method used

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  • Application of long-chain non-coding RNA lncLCIR-1 as lung cancer molecular marker
  • Application of long-chain non-coding RNA lncLCIR-1 as lung cancer molecular marker
  • Application of long-chain non-coding RNA lncLCIR-1 as lung cancer molecular marker

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Experimental program
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Effect test

Embodiment 1

[0036] Example 1. Changes in the expression of non-coding RNA in lung cancer cells after radiotherapy

[0037] Detect the expression changes of non-coding RNA in lung cancer cells after radiotherapy, and determine the non-coding RNA closely related to lung cancer radiotherapy by detecting the relative expression of non-coding RNA in tissue samples of lung cancer patients.

[0038] 1. Radiotherapy dose that is half lethal for lung cancer cells

[0039] The human lung cancer cell lines A549, H460, H1975, H1299 were recovered and cultured, and the cells were cultured to the logarithmic growth phase. The cells were trypsinized and suspended in 1640 medium for cell counting. Take different cell gradients (500, 800, 1000, 1500, 2000 / well) and spread them in a 96-well plate to ensure that the cells have sufficient nutrients, observe the cell growth status, and select the cell confluence of 80-90 after 1 week % Of the number of cells plated. After determining the number of plated cells, sp...

Embodiment 2

[0053] Example 2. Effects of nucleic acid molecules on lung cancer cells

[0054] Use small interfering RNA and overexpression plasmid vector to transiently transfect different lung cancer cells to change the expression of lncLCIR-1, and explore the effect of lncLCIR-1 on lung cancer cell proliferation, clone formation, cycle and invasion biological functions.

[0055] 1. The background expression level of lncLCIR-1 in lung cancer cells

[0056] RT-qPCR was used to detect the background expression level of lncLCIR-1 in A549, H460, H1975, H1299 cells, and RNA from A549, H460, H1975, H1299 cells was extracted, and RT-qPCR was performed after reverse transcription.

[0057] RT-qPCR reaction system: 2×SYBR Premix Ex Taq (TliRNaseH Plus) 5μL, 50×ROXReference Dye 0.04μL, upstream and downstream primers (10μM) 0.5μL, cDNA 0.6μL, ultrapure water 3.86μL, total 10μL.

[0058] RT-qPCR reaction conditions: 94℃15s; 95℃5s, 60℃30s, 45 cycles; 72℃10min, 4℃1min.

[0059] Among them, the upstream and down...

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Abstract

The invention relates to an application of long-chain non-coding RNA lncLCIR-1 as a lung cancer molecular marker. According to the invention, stable high expression of the lncLCIR-1 in lung cancer tissues is found for the first time, and the level of the lncLCIR-1 is reduced after radiotherapy, so that the lncLCIR-1 in the lung cancer is a potential index for predicting the curative effect of theradiotherapy, and is a novel lung cancer molecular marker. The application of the lncLCIR-1 detection reagent in preparing a reagent for diagnosing the prognosis of a lung cancer patient mainly uses the lncLCIR-1 as a detection marker to prepare a corresponding fluorescence quantitative PCR detection kit for detecting the prognosis of the lung cancer patient in radiotherapy. In addition, the siRNAcan inhibit the expression of lncLCIR-1, so that the cell cycle of the lung cancer is regulated, the G1 phase block of the lung cancer cell is caused, the proliferation of the lung cancer cell is inhibited, and the siRNA has important significance in the preparation of a medicament for treating the lung cancer.

Description

Technical field [0001] The invention relates to the application of a long-chain non-coding RNA lncLCIR-1 as a molecular marker for lung cancer, and belongs to the technical field of molecular biology. Background technique [0002] At the moment when science and technology are extremely advanced, malignant tumors are still a major killer threatening human life and health, and lung cancer is a common malignant tumor worldwide, and it is also the tumor with the highest morbidity and mortality in my country. As a common and effective treatment for tumors, radiotherapy plays an important role in the treatment of lung cancer. However, the long-term survival rate of patients still needs to be improved. In addition, there are individual differences in the sensitivity of different patients to radiotherapy, and some patients even develop resistance to radiotherapy during treatment, and there is a lack of clinically unified molecular markers to predict the efficacy and prognosis of patients...

Claims

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Application Information

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IPC IPC(8): C12Q1/6886C12Q1/6851A61K31/713A61P35/00
CPCC12Q1/6886C12Q1/6851A61K31/713A61P35/00C12Q2600/118C12Q2600/158C12Q2600/166C12Q2531/113C12Q2563/107C12Q2545/101
Inventor 杨明寇培思张娜莎
Owner SHANDONG RES INST OF TUMOUR PREVENTION TREATMENT
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