Bifidobacterium pseudocatenulatum CCFM1047, composition, fermented food, application and bacterial agent of bifidobacterium pseudocatenulatum CCFM1047 and preparation method of bacterial agent of bifidobacterium pseudocatenulatum CCFM1047

A bifidobacterium and fermented food technology, applied in the field of microorganisms, can solve the problems of stimulating the gastrointestinal tract, liver function damage, affecting patients, etc., and achieve the effect of alleviating weight gain, alleviating metabolic syndrome, and improving content increase

Active Publication Date: 2019-08-09
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These drugs have a good curative effect on each single risk factor of metabolic syndrome. However, long-term use of drugs has certain side effects. For example, long-term use of metformin can cause irritation to the gastrointestinal tract of some patients and cause discomfort and may affect the patient’s response to vitamin B12. The absorption of rosiglitazone can cause liver function damage and edema, etc.

Method used

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  • Bifidobacterium pseudocatenulatum CCFM1047, composition, fermented food, application and bacterial agent of bifidobacterium pseudocatenulatum CCFM1047 and preparation method of bacterial agent of bifidobacterium pseudocatenulatum CCFM1047
  • Bifidobacterium pseudocatenulatum CCFM1047, composition, fermented food, application and bacterial agent of bifidobacterium pseudocatenulatum CCFM1047 and preparation method of bacterial agent of bifidobacterium pseudocatenulatum CCFM1047
  • Bifidobacterium pseudocatenulatum CCFM1047, composition, fermented food, application and bacterial agent of bifidobacterium pseudocatenulatum CCFM1047 and preparation method of bacterial agent of bifidobacterium pseudocatenulatum CCFM1047

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0084] Example 1: Pseudo-small chain Bifidobacterium CCFM1047 has good tolerance to simulated gastrointestinal fluid

[0085] Inoculate the cryopreserved Bifidobacterium CCFM1047 in mMRS medium (MRS medium + 0.05% cysteine ​​hydrochloride), culture anaerobically at 37°C for 48 hours, and then subculture in mMRS medium for 2 After ~3 times, take 1mL of the culture solution of Bifidobacterium pseudospermia CCFM1047, mix it with 9.0mL of pH 2.5 artificial simulated gastric juice (mMRS medium containing 1% pepsin, pH=2.5), and anaerobically For culture, samples were taken at 0h, 0.5h, 1h and 2h respectively, poured and cultured with mMRS agar medium to count plate colonies, determine the number of viable bacteria and calculate their survival rate.

[0086] The survival rate is the ratio of the logarithmic value of the number of viable bacteria at the time of sampling to the logarithmic value of the number of viable bacteria at the 0th hour in the culture solution, expressed in %. ...

Embodiment 2

[0091] Example 2: Pseudomonas Bifidobacterium CCFM1047 has no toxic side effects on C57BL / 6J mice

[0092] Pseudomonas bifidobacterium CCFM1047 cell resuspended in 3% sucrose solution to make a concentration of 3.0 × 10 8 CFU / mL bacterial suspension. Six healthy male C57BL / 6J mice with a body weight of about 20 g were taken. After one week of adaptation to the environment, the bacterial suspension of this concentration was administered orally once a day, observed for one week, and the death and body weight were recorded.

[0093] The results of these tests are listed in Table 3. These results indicate that feeding concentrations of 3.0×10 8 The CFU / mL pseudosmall chain Bifidobacterium CCFM1047 had no significant impact on mice, no significant change in body weight, and no death. The appearance of the mice had no obvious pathological symptoms.

[0094] Table 3 Changes in body weight and death of mice

[0095]

[0096] Note: -: no death of mice

Embodiment 3

[0097] Example 3: Pseudomonas bifidobacterium CCFM1047 has a recovery effect on the intestinal flora imbalance caused by high-fat feed

[0098] 36 healthy male C57BL / 6J mice weighing 18-20 g were taken, adapted to the environment for 1 week, and randomly divided into 6 groups: blank control group (NC), high-fat model control group (HFD), simvastatin control group (SC ), rosiglitazone control group (RH), Bifidobacterium pseudospermia CCFM1047 intervention group (CCFM1047), Bifidobacterium animalis BB12 control group (BB12), each group contained 6 mice, and the bacteria suspension The dose is 3.0 x 10 8 CFU / mL, resuspended in 3% sucrose solution. The grouping and treatment methods of experimental animals are shown in Table 4:

[0099] Table 4 Grouping of experimental animals

[0100]

[0101] At the end of the experiment, the fresh feces of the mice were collected and frozen at -80°C, the metagenome in the feces was extracted, and the structure of the intestinal flora was ...

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Abstract

The invention discloses bifidobacterium pseudocatenulatum CCFM1047, a composition, fermented food, application and bacterial agent of the bifidobacterium pseudocatenulatum CCFM1047 and a preparation method of the bacterial agent of the bifidobacterium pseudocatenulatum CCFM1047. The bifidobacterium pseudocatenulatum CCFM1047 can tolerate the gastrointestinal environment of the human body, significantly improve abnormal fasting blood glucose and abnormal oral glucose tolerance of a mouse with the metabolic syndrome, reduce the area under the drug-time curve of glucose tolerance, reduce the serum insulin level, significantly relieve the weight rise of the mouse, significantly relieve the rise, caused by the high fat diet, of content of low density lipoprotein cholesterol in serum and triglyceride in the liver of the mouse with the metabolic syndrome and significantly relieve the rise, caused by the high fat diet, of Proteus in the intestinal tract; the bifidobacterium pseudocatenulatum CCFM1047 is used for preparing the medicine composition and the fermented food which relieve the metabolic syndrome and reduce the occurrence of diseases such as the Parkinson disease, intestinal infection and urinary tract and kidney infection and has a quite wide application prospect.

Description

technical field [0001] The invention belongs to the technical field of microbes, and in particular relates to pseudosmall chain bifidobacterium CCFM1047, its composition, fermented food, application, bacterial agent and a preparation method thereof. Background technique [0002] In recent years, with the continuous development of our country's social and economic level, the living standards of the public have continued to improve, the proportion of carbohydrates and fats in the diet structure has increased day by day, and the restrictions of many working conditions have made it easy for people to develop sedentary behavior Habit. These external environmental factors combined with their own genetic factors have led to the continuous increase in the incidence of metabolic syndrome in the Chinese population. According to reports, between 2002 and 2010, the proportion of people over the age of 20 in my country with metabolic syndrome rose from 5.4% to 21.3%, an increase of 4 ti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A61K35/745A61P3/06A61P3/10A61P1/16A61P25/16A61P31/00A23C9/123A23C13/16A23C19/032A23C11/10A23L11/00A23L33/00C12R1/01A23L11/50
CPCC12N1/20A61K35/745A61P3/06A61P3/10A61P1/16A61P25/16A61P31/00A23C9/1234A23C13/16A23C19/0323A23C11/106A23L33/00A61K2035/115A23V2002/00A23L11/50C12R2001/01C12N1/205A23V2400/535A23V2200/3204A23V2200/3262A23V2200/328A23V2200/32A23V2200/322A23V2200/30
Inventor 陈卫王刚刘煜飞赵建新张灏
Owner JIANGNAN UNIV
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