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Method for detecting florfenicol amine in milk or dairy product

A technology of florfenicolamide and a detection method, which is applied in the detection field of florfenicolamide and can solve the problems that the detection method of florfenicolide needs to be studied and the like

Inactive Publication Date: 2019-08-09
INNER MONGOLIA MENGNIU DAIRY IND (GRP) CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, the current method for the detection of florfenicol amine in milk or milk products remains to be studied

Method used

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  • Method for detecting florfenicol amine in milk or dairy product

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] In this example, florfenicol amine in milk or milk products was detected according to the following method:

[0027] 1 Reagents and materials

[0028] 1.1 The purity of florfenicol amine is greater than or equal to 98%.

[0029] 1.2 Chromatographically pure ethyl acetate, analytically pure ammonia (20-30%).

[0030] 1.3 Florfenicol amine standard stock solution (100 μg / mL): Weigh 0.01 g of the standard florfenicol amine, dissolve it in acetonitrile water (volume ratio 80:20) to 100 mL, the concentration of this standard solution is 100 μg / mL mL.

[0031] 1.4 Florfenicol amine standard intermediate solution 1.0 μg / mL: Take 1.0 mL of florfenicol amine standard stock solution in a 100.0 mL volumetric flask, dissolve it with acetonitrile water (volume ratio 80:20), and this standard intermediate solution The concentration was 1.0 μg / mL.

[0032] 2 Instruments and equipment

[0033] 2.1 Liquid chromatography-mass spectrometry / mass spectrometry.

[0034] 2.2 Chromatogra...

Embodiment 2

[0064] In this embodiment, compare the impact of different treatment reagents on the detection results:

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Abstract

The invention provides a method for detecting florfenicol amine in milk or a dairy product. The method comprises the steps: (1) the milk or the dairy product, acetonitrile and ammonium hydroxide are mixed and centrifuged, and supernatant is collected and dried, so that a dried object is obtained; (2) the dried object is dissolved through an alcoholic solution, a dissolved solution is purified, andan obtained purified solution is collected and dried, then dissolved through acetonitrile and filtered, so that a to-be-detected solution is obtained; and (3) the to-be-detected solution is detectedthrough liquid chromatography-mass spectrometry. The detection method has the advantages of being high in accuracy, high in sensitivity, quick, easy and convenient to operate and the like, and is suitable for being widely applied.

Description

technical field [0001] The invention relates to the field of food. Specifically, the present invention relates to a method for detecting florfenicol amine in milk or milk products. Background technique [0002] Florfenicol amine is a metabolite of florfenicol. The European Union stipulates the maximum residue limit of florfenicol in food of animal origin, and florfenicol amine is used as the detected object. [0003] However, the current detection method of florfenicol amine in milk or milk products remains to be studied. Contents of the invention [0004] The present invention aims to solve at least one of the technical problems existing in the prior art at least to a certain extent. [0005] For this reason, the present invention proposes a method for detecting florfenicol amine in milk or milk products. According to an embodiment of the present invention, the detection method includes: (1) mixing and centrifuging milk or milk products with acetonitrile and ammonia wa...

Claims

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Application Information

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IPC IPC(8): G01N30/88G01N30/06
CPCG01N30/06G01N30/88
Inventor 李照常建军宋晓东喻东威梁春梅徐向峰袁凤琴马若影张利俊
Owner INNER MONGOLIA MENGNIU DAIRY IND (GRP) CO LTD
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