A kind of heterocyclic amine 8-meiqx hapten, antibody and its preparation method and application

An 8-meiqx, 21mg8-meiqx technology, applied in the field of small molecule compound immunochemistry and trace residue analysis, can solve the problems of complex meat products, not widely used, high instrument dependence, and achieve rapid enzyme-linked immunoassay detection. method, reliable technical support, high affinity effect
CN110117286BActive Publication Date: 2020-07-24TIANJIN UNIV OF SCI & TECH
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
TIANJIN UNIV OF SCI & TECH
Publication Date
2020-07-24

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Abstract

The invention provides a heterocyclic aromatic amine 8-MeIQx hapten and antibody and a preparing method and application of the antibody, and relates to synthesis of heterocyclic aromatic amine haptens, preparation of artificial complete antigens and antibodies and building of ensyme-linked immunoassay methods of the antibodies. According to the heterocyclic aromatic amine 8-MeIQx hapten and antibody and the preparing method and application of the antibody, aiming at the requirements for high throughput of heterocyclic aromatic amine detection and high sample preparation speed, a sensitive, efficient, quick and accurate immunoassay technology is built and solves the problems that existing physical-chemical analysis methods are cumbersome, complicated, high in cost and low in analysis speed.The method for detecting heterocyclic aromatic amines in food is built with the antibody, has good sensitivity, can simultaneously recognize eight polarity heterocyclic aromatic amines including 8-MeIQx, and has good compatibility, and the detection limit (in terms of 8-MeIQx) is 10.92 microgram / L; meanwhile, the detection method is low in cost, easy to operate and suitable for quick detection ofthe total quantity of the polarity heterocyclic aromatic amines in the food.
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Description

technical field

[0001] The invention belongs to the technical field of immunochemistry and trace residue analysis of small molecule compounds; relates to organic synthesis, immunochemistry, biochemistry and physical and chemical testing technology, etc.; especially relates to the design and synthesis of small molecule compound heterocyclic amine 8-MeIQx hapten and artificial antigen And the preparation of specific antibody of immunized animals and the establishment of immunoassay method. Background technique

[0002] Heterocyclic amines (HAAs) are a class of polycyclic aromatic compounds containing N-heterocycles, which are produced by the high-temperature reaction of free amino acids, sarcosine, creatinine and carbohydrates, and have carcinogenic and mutagenic activities. Heterocyclic amines are widely found in fried foods, coffee drinks, alcohol, cigarette smoke, human body fluids, river water and the atmosphere. So far, more than 25 heterocyclic amines have been isolated...

Claims

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