Naphthalene-degrading strain applied to sewage treatment and microbial agent and application thereof

Abstract

The invention relates to the technical field of biodegradation in sewage treatment technologies, in particular to a naphthalene-degrading stain applied to sewage treatment. The naphthalene-degrading strain comprises bacillus subtilis, corynebacterium and rhodococcus. An incubator cultivation method comprises the steps that s1, degrading bacteria are screened, water samples are collected from petroleum-polluted seaports, and the bacillus subtilis, the corynebacterium and the rhodococcus are screened out; s2, strain domestication is conducted. The invention further discloses a microbial agent ofthe naphthalene-degrading strain applied to sewage treatment. The microbial agent comprises the bacillus subtilis, the corynebacterium and the rhodococcus, and the total viable bacteria number is 1.5*10<8>-6.0*10<8> cfu / mL. The invention further discloses application in inoculating the microbial agent of the naphthalene-degrading strain into a filler and applying the filler in a biodegrading pondfor degrading naphthalene in sewage. The biodegradable bacteria have the efficient and high degradation speed on naphthalene, the survival rate of the naphthalene-degrading strain in high concentration naphthalene-containing sewage is high, the treatment effect is good, the treatment speed is high, and secondary pollution does not exist.

Description

technical field [0001] The invention relates to the technical field of biodegradation in sewage treatment technology, in particular to a naphthalene-degrading bacterium used in sewage treatment and its agent and application. Background technique [0002] Naphthalene is an organic compound that is volatile and has a special fragrance. It is mainly used in chemical products such as synthetic fuels, resins, textiles, and plastics. It is a common chemical raw material. Naphthalene is also widely distributed in oil-polluted seaports, soil, garbage collection and distribution, etc. Naphthalene is a kind of organic pollutant widely present in wastewater. Organic substances contained in wastewater include phenol, naphthalene, organic solvents, etc. Among them, naphthalene is a common chemical raw material, and naphthalene is highly toxic, and sewage is easy to stink. The current commonly used treatment methods for sewage containing naphthalene include: (1) physical and chemical met...

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Problems solved by technology

[0005] The technical problem to be solved by the present invention is: 1, the singleness problem of biodegradation bacteria, because naphthalene has high toxicity, the activity of most biodegradation bacteria is relatively poor, needs to effectively screen out the microorganism that degrades naphthalene, simultaneously naphthalene will have Microorganisms produce certain resistance and reduce the degradation speed and effect of degrading bacteria. Therefore, effectively improving the survival time and survival activity of degrading bacteria in organic wastewater is the main problem at present; 2. Degrading bacteria in the complex environment of sewage , the degradative activity of the strains screened in the laboratory on naphthalene in sewage will decrease, and they cannot grow or achieve the predetermined effect in a complex environment; 3. The common degradation method of degrading bacteria is to cultivate degrading bacteria in activated sludge, Then decompose naphthalene by the metabolism of microorganisms, the cultivation time is long, and the screening process of the degrading bacteria in the prior art is slow, and the screening effect is poor, and the metabolism of the degrading bacteria is greatly affected by the environment such as temperature and nutrients, and the treatment effect is relatively low. Slow, resulting in long sewage treatment time

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