Determination method of quantity of cassava pollen
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A method of determination, pollen technology, applied in the direction of measuring devices, individual particle analysis, scientific instruments, etc., can solve the problems that cannot be counted by hemocytometer method, achieve the effect of increasing the viscosity control, improving accuracy, and simple operation
Inactive Publication Date: 2019-08-16
SOUTH ASIAN TROPICAL AGRI SCI RES INST OF GUANGXI
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[0005] The object of the invention is to provide a method for measuring the quantity of cassava pollen, which overcomes the def
Method used
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Embodiment 1
[0017] In early October 2018, new selection 048 cassava pollen was collected at the cassava base of Guangxi South Subtropical Agricultural Science Research Institute in Binqiao Township, Longzhou County, Chongzuo City, Guangxi Province, and the number of cassava pollen was measured in the laboratory.
[0018] A method for measuring cassava pollen quantity, comprising the following steps:
[0019] (1) Take 10 cassava anthers that are fully mature, plump and uncracked, put them into a clean 2ml centrifuge tube, place them in a desiccator, and accelerate cracking in a 30°C incubator;
[0020] (2) After the pollen is completely dispersed, add 2ml of dispersion mixture, vibrate and shake on a vortex shaker to form suspended pollen liquid; said dispersion suspension mixture: Group A: composed of 20% sodium metaphosphate, 2% sucrose , 2% glucose, 0.01% hydroxypropyl methylcellulose, 0.05% xanthan gum, and the balance is composed of water;
[0021] Group B: composed of 20% sodium met...
Embodiment 2
[0027] In early November 2018, new selection 048 cassava pollen was collected at the cassava base of Guangxi South Subtropical Agricultural Science Research Institute in Binqiao Township, Longzhou County, Chongzuo City, Guangxi Province, and the number of cassava pollen was measured in the laboratory.
[0028] A method for measuring cassava pollen quantity, comprising the following steps:
[0029] (1) Take 10 cassava anthers that are fully mature, plump and uncracked, put them into a clean 2ml centrifuge tube, place them in a desiccator, and accelerate cracking in a 30°C incubator;
[0030] (2) After the pollen is completely scattered, add 2ml of the dispersion mixture, and oscillate on a vortex shaker to form a suspended pollen liquid.
[0031] The dispersion suspension mixture of the experimental group is composed of 20% sodium metaphosphate, 2% sucrose, 2% glucose, 0.01% hydroxypropyl methylcellulose, 0.04%-0.06% xanthan gum, and the balance is water.
[0032] Control grou...
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Abstract
The invention discloses a determination method of the quantity of cassava pollen. The method includes steps: taking and putting 10 fully mature, plump and uncracked cassava anthers into a 2 ml centrifugal tube, and placing the centrifugal tube in a dryer in an incubator at the temperature of 30 DEG C for accelerating cracking; after completion of spilling of pollen, adding dispersing mixing liquid, and performing uniform oscillation on a vortex oscillator to form suspended pollen liquid, wherein the dispersing suspended mixing liquid is formed by 20% of sodium metaphosphate, 2% of sucrose, 2%of glucose, 0.01% of hydroxypropyl methyl cellulose, 0.04%-0.06% of xanthan gum, and the remaining water; and taking and dripping the suspended pollen liquid on a slide, and performing observation andstatistics on the particle quantity of pollen under a microscope. According to the method, the condition that the particle size of the cassava pollen is greater than 0.10 mm so that the counting cannot be performed by employing a blood counting chamber determination method for determination of the pollen quantity is overcome; besides, through selection of proper dispersing mixing liquid, the stability of the pollen suspension liquid is improved, the deposition speed of pollen is controlled, and observation is easier and counting is more accurate during determination by adopting a microscope observation method.
Description
technical field [0001] The invention belongs to the technical field of cassava detection, in particular to a method for measuring the number of cassava pollen. Background technique [0002] The role of pollen count measurement: [0003] In the existing literature, the pollen count of many crops is measured by a hemocytometer. This is usually because the diameter of the pollen grains of this species or variety is less than 0.10mm, which can smoothly enter the hemocytometer, so that the pollen count can be effectively measured. Observe, and then estimate the actual amount of pollen in each anther. However, there are very few reports in the literature on the measurement of cassava pollen. There is only one article that mentions that cassava pollen is measured by a hemocytometer. However, the actual results of the applicant's repeated tests found that cassava pollen cannot enter the hemocytometer through siphoning. , resulting in significant discrepancies between the measured ...
Claims
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IPC IPC(8): G01N15/10
CPCG01N15/10G01N2015/105G01N2015/1062
Inventor 何文李恒锐张秀芬杨海霞刘连军黎萍梁振华黄珍玲谢君锋韦雪英
Owner SOUTH ASIAN TROPICAL AGRI SCI RES INST OF GUANGXI
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