Preparation method of fermented yellow distiller's grains for feeding and fermented yellow distiller's grains thereof
A technology for rice distiller's grains and feeding, which is applied in applications, animal feed, animal feed, etc., to achieve the effects of improving structure, increasing bionic digestibility, and reducing content
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Embodiment 1
[0021] Example 1 A preparation method of laboratory fermented yellow distiller's grains
[0022] Take 20 g of substrate (80% distiller's grains and 20% wheat bran), inoculate with 3% (v / w) (1.0 x 10 8 CFU / ml) of Bacillus subtilis CW4 mixed inoculation. The substrate was fermented in a 100 mL Erlenmeyer flask, covered with a sterile film (aerobic condition), and sterile water was added on a dry basis to make the moisture content reach 40%. The substrate was fermented at 37°C for 36 h. After the first stage of fermentation, the fermentation mix was inoculated with 3% (v / w) (1.0x10 8 CFU / ml) of Pediococcus pentosaceae CWPP, a rubber stopper (anaerobic condition) was placed on the bottle mouth, and after 48 hours of fermentation at 37°C under anaerobic conditions, some wet samples were taken for microbial metabolites and in vitro digestibility Quick check. The rest of the samples were treated at 105°C for 20 minutes to terminate the fermentation. The samples were then dried ...
Embodiment 2
[0033] Example 2 Fermentation of rice distiller's grains
[0034] Bacillus culture medium: soybean meal 1%, yeast extract 0.5%, brown sugar 0.5%, potassium dihydrogen phosphate 0.1%. Anaerobic medium; lactic acid bacteria medium: tomato powder 1%, yeast extract 0.5%, brown sugar 0.5%, potassium dihydrogen phosphate 0.1%.
[0035] Activation of the Bacillus fermentation broth: cool down the aerobic culture medium that has sterilized the bacteria to about 40°C, and insert the activated Bacillus. Cultivate in a constant temperature shaker for 24 hours to obtain a Bacillus fermentation broth. Activation of lactic acid bacteria fermentation broth: cool down the sterilized anaerobic medium to about 40°C, and insert the activated lactic acid bacteria. 37 ℃ constant temperature incubator static culture for 24 hours to obtain lactic acid bacteria fermentation broth.
[0036] According to 18Kg of raw materials (80% of rice wine wet grains, 20% of bran), the activated Bacillus and lac...
Embodiment 3
[0040] Example 3 Pilot-scale fermentation of rice distiller's grains
[0041] 1. Preparation of feed raw material immersion juice (activated medium): by mass percentage, 5% rice wine lees powder, 0.8% Na 2 HPO 4 12H 2 O, 0.4% (NH 4 ) 2 SO 4 , 0.15% NH 4 Cl, add water and stir for 10 min. After the preparation is complete, heat the culture medium to 90°C and cool it down to room temperature naturally.
[0042] 2. Preparation of secondary fermentation seed liquid: inoculate freshly cultivated Bacillus subtilis CW4 and Pediococcus pentosaceae CWPP into 5 mL LB and MRS liquid medium respectively, cultivate CW4 for 4-6 hours, and CWPP for 24 hours to make OD600 reach 0.5, add 1mL of cultured primary seed liquid to the final mass of 2%~3% into the feed material soaking juice respectively at 37°C for 8 hours and 12 hours, respectively, so that the OD 600 When it reaches 0.5, get the respective secondary seed solutions for later use.
[0043] 3. Fermentation of yellow distille...
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