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Method for screening Cobb broiler DNA barcode and application of DNA barcode

A barcode, broiler technology, applied in the field of molecular biology, can solve the problems of unfavorable popularization and application, complicated operation and high cost

Active Publication Date: 2019-08-20
GUANGXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Therefore, based on the variation information of the mitochondrial D-loop region sequence to evaluate the genetic relationship of the variety and whether there is any introgression of other varieties, although the use of microsatellite molecular markers and the sequence of the mitochondrial D-loop region can accurately determine whether the variety is mixed with the lineage of foreign varieties , but these methods are complicated to operate, time-consuming, and costly, which is not conducive to popularization and application in production

Method used

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  • Method for screening Cobb broiler DNA barcode and application of DNA barcode
  • Method for screening Cobb broiler DNA barcode and application of DNA barcode
  • Method for screening Cobb broiler DNA barcode and application of DNA barcode

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Experimental program
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Effect test

Embodiment 1

[0033]In order to screen the specific DNA barcode fragments of Kebao broilers, this method uses Xiayan chicken, Lingyun black-bone chicken, Donglan black-bone chicken, Guangxi Ma chicken, Lingshan Ma chicken, Lingshan fragrant chicken, Guangxi Sanhuang chicken, Longsheng Feng chicken, Nandan Yao chicken , Beijing oil chicken, Xuefeng black-bone chicken, camellia chicken, Aiweiyin chicken, Kebao broiler chicken and Bailaihang chicken and other 15 breeds were used as the research objects, among which Xiayan chicken was taken from Zhouyi Farm in Rong County, and Lingyun black-bone chicken was taken from From the breeding farm of Chenshi Animal Husbandry Co., Ltd. in Lingyun County, Guangxi, Donglan black-bone chicken is from the original breeding farm of Donglan black-bone chicken in Donglan County, Guangxi Ma chicken is from the breeding farm of Fufeng Agriculture and Animal Husbandry Co., Ltd. in Nanning, Guangxi The chicken was taken from the breeding farm of Yuanfeng Animal Hu...

Embodiment 2

[0045] Cobb Broiler Specific Sequence Large Population Verification

[0046] The unique haplotype sequence of the Kebao broiler chicken obtained in Example 1 is used as the standard for whether the local chicken is mixed with the lineage of the Kebao broiler chicken:

[0047] Congrong County Zhouyi Breeding Farm, Guangxi Lingyun County Chen’s Animal Husbandry Co., Ltd. Breeding Farm, Donglan Black-bone Chicken Original Breeding Farm, Guangxi Nanning City Fufeng Agriculture and Animal Husbandry Co., Ltd. Breeding Farm, Guangxi Lingshan County Yuanfeng Animal Husbandry Co., Ltd. The company's seed conservation farm, Guangxi Lingshan County Xingmu Animal Husbandry Co., Ltd. seed conservation field, Guangxi Chunmao Agriculture and Animal Husbandry Group Co., Ltd. seed conservation field, Guangxi Longsheng County Hongsheng Poultry Industry Co., Ltd. Blood samples of Xiayan chicken, Lingyun black chicken, Donglan black chicken, Guangxi Ma chicken, Lingshan Ma chicken, Lingshan fragr...

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Abstract

The invention discloses an application of a Cobb broiler DNA barcode 'TCTACGGGC' in screening Cobb broilers, and the Cobb broiler DNA barcode is obtained by the screening method. SNP sites and haplotype sequences specific on 12S rRNA are screened from mitochondrial genes by a PCR direct sequencing method by use of bioinformatic analysis software, one DNA barcode sequence specific to the Cobb broilers is obtained by large-group verification, and the DNA barcode breaks through the defects of long detection period and high cost of traditional microsatellite molecular markers, can rapidly and conveniently detect whether the blood lineage of the Cobb broilers is mixed in local varieties and can be applied to verification analysis of Cobb broiler purebreds.

Description

technical field [0001] The invention relates to the technical field of molecular biology, in particular to a method for screening Kebao broiler DNA barcodes and the application of the DNA barcodes. Background technique [0002] Guangxi has rich germplasm resources of local chickens. Currently, there are 6 local chicken breeds in Guangxi listed in the China Poultry Breed Resources List, namely Guangxi Sanhuang Chicken, Xiayan Chicken, Guangxi Ma Chicken (Lingshan Fragrant Chicken and Lidang Chicken), Nan Danyao chicken, Guangxi black-bone chicken (Donglan black-bone chicken and Lingyun black-bone chicken), Longsheng Phoenix chicken. These local breeds in Guangxi have excellent meat and egg quality. Guangxi Sanhuang is a meat-egg variety. The number of chickens raised ranks first in the country for high-quality broilers. The annual production and sales exceed 350 million. The meat is tender and has a strong smell. Nandanyao chicken is a meat and egg variety with excellent cha...

Claims

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Application Information

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IPC IPC(8): C12Q1/6888C12Q1/6869C12N15/11
CPCC12Q1/6888C12Q1/6869C12Q2600/156C12Q2600/158C12Q2600/178C12Q2531/113
Inventor 杨秀荣邓继贤杨祝良
Owner GUANGXI UNIV
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