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Phage and application thereof in preparation of photodynamic preparation for inactivating bacteria

The technology of bacteriophage and photosensitizer is applied in the application field of photodynamic preparations, which can solve the problems of complicated preparation steps, increase biological toxicity, etc., and achieve the effects of high biological safety, efficient sterilization and good sterilization effect.

Active Publication Date: 2019-08-23
TECHNICAL INST OF PHYSICS & CHEMISTRY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The preparation steps of this method are relatively complicated, and the introduction of organic solvents in chemical synthesis will also increase its biological toxicity

Method used

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  • Phage and application thereof in preparation of photodynamic preparation for inactivating bacteria
  • Phage and application thereof in preparation of photodynamic preparation for inactivating bacteria

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preparation example Construction

[0036] In a preferred embodiment of the present invention, when the photosensitizer is a positively charged photosensitizer (such as TPE-Pt-MC), the weak interaction between the photosensitizer and the phage can be an electrostatic interaction, At this time, the preparation method of the phage attached to the photosensitizer includes the following steps: mixing the phage with the photosensitizer with aggregation-induced luminescent properties, and obtaining the phage with the photosensitizer adsorbed on the outer surface through electrostatic interaction. Further preferably, the bacteriophage and the photosensitizer are mixed according to the ratio of the amount of substances being 1:0.5-1:50.

[0037] When the weak interaction between the photosensitizer and the phage is a hydrophilic-hydrophobic interaction, and the photosensitizer is a hydrophobic photosensitizer (such as ZnPc, Ce6, hematoporphyrin, etc.), the preparation method of the phage attached to the photosensitizer i...

Embodiment 1

[0042] Obtain M13 bacteriophage by the method of prior art, its TEM photo is attached figure 1 shown. Mix the M13 bacteriophage with a ratio of 1:2 and the TPE-based photosensitizer (TPE-Pt-MC) with aggregation-induced luminescent properties, and obtain the surface-adsorbed photosensitizer TPE-Pt-MC through electrostatic interaction. M13 phage, namely TPE-Pt-MC@M13. TPE-Pt-MC@M13 was used for M13-specific targeted bacteria for antibacterial experiments.

Embodiment 2

[0044] The T2 phage is obtained by the method of the prior art, and the T2 phage with a ratio of 1:1 of substances is mixed with a TPE-based photosensitizer (TPE-Pt-MC) with aggregation-induced luminescent properties, and the outer surface is obtained by electrostatic interaction. T2 phage with photosensitizer TPE-Pt-MC adsorbed on the surface, namely TPE-Pt-MC@T2. TPE-Pt-MC@T2 was used for T2-specifically targeted bacteria for antibacterial experiments.

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Abstract

The invention discloses a phage and application thereof in preparation of a photodynamic preparation for inactivating bacteria and relates to the technical field of nano novel materials. The surface of the phage is connected with a photosensitizer through weak interaction, the weak interaction can be electrostatic interaction or hydrophobic interaction, and the photosensitizer can be specificallytargeted to certain bacteria through specific interaction between the phage and the bacteria. The invention also discloses application of the photosensitizer attached phage in preparation of the photodynamic preparation for inactivating bacteria.

Description

technical field [0001] The invention relates to the technical field of new nanometer materials. More specifically, it relates to a phage and its application in the preparation of photodynamic preparations for inactivated bacteria. Background technique [0002] Bacterial infection poses a serious threat to human health, and the emergence of bacterial resistance caused by the abuse of antibiotics has made this problem more and more serious. Due to the characteristics of non-invasiveness and high spatiotemporal selectivity, photodynamic sterilization has attracted extensive attention. Photodynamic sterilization consists of three parts: photosensitizer, light source and oxygen. These three components are non-toxic when they exist alone. The photosensitizer can transfer energy or electrons to oxygen under the irradiation of a light source, thereby being able to generate reactive oxygen species, such as singlet oxygen ( 1 o 2 ), superoxide anion (O 2 ·- ) and hydroxyl radica...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K41/00A61K47/51A61P31/04C12N7/00C12R1/92
CPCA61K41/0057A61K41/0071A61K47/51A61P31/04C12N7/00C12N2795/10121C12N2795/10132C12N2795/14121C12N2795/14132
Inventor 牛忠伟田野高偲嘉
Owner TECHNICAL INST OF PHYSICS & CHEMISTRY - CHINESE ACAD OF SCI