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A targeted copper-doped carbon dot and its preparation method and application

A nitrogen-doped carbon dot and copper-doped technology, applied in pharmaceutical formulations, photodynamic therapy, preparations for in vivo experiments, etc., can solve the problem that copper-based nanomaterials fail to reach cancer cells accurately and copper-based nanomaterials fail Targeted modification, photothermal, and photodynamic therapy have not been reported to achieve good photothermal conversion efficiency, good photosensitivity, and good targeting.

Active Publication Date: 2021-10-15
GUANGXI NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] In recent years, the synthesis of multifunctional copper-based nanomaterials has made great progress, but most of the synthesized copper-based nanomaterials have not been targetedly modified, which makes it impossible for copper-based nanomaterials to accurately reach cancer cells during application. There is blindness in the cell location and phototherapy process. At the same time, the photothermal and photodynamic therapy mediated by copper-doped carbon dots in response to tumor microenvironment fluorescence imaging have not yet been reported.

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  • A targeted copper-doped carbon dot and its preparation method and application
  • A targeted copper-doped carbon dot and its preparation method and application
  • A targeted copper-doped carbon dot and its preparation method and application

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preparation Embodiment 1

[0031] The preparation of targeted copper-doped carbon dots comprises the following steps:

[0032] S1. Weigh 100 mg of hyaluronic acid and 200 mg of polyethyleneimine, add 20 ml of distilled water, stir to make it dissolve completely and become a transparent liquid;

[0033] S2. Put the above solution in a reaction kettle for hydrothermal reaction, the hydrothermal temperature is 180°C, and the reaction time is 6 h;

[0034] S3. Filtrating the liquid obtained by the hydrothermal method with a microporous filter membrane with a pore size of 0.22 μm to obtain an aqueous solution of nitrogen-doped carbon dots;

[0035] S4. Mix and stir 20 ml of the nitrogen-doped carbon dot aqueous solution obtained in S3 with 0.1 M, 20 ml of copper ions, and dialyze for 72 h with a cellulose dialysis bag with a molecular weight cut-off of 500-1000 to obtain targeted copper-doped carbon dots Aqueous solution of , after freeze-drying, solid targeted copper-doped carbon dots were obtained.

preparation Embodiment 2

[0037] The preparation of targeted copper-doped carbon dots comprises the following steps:

[0038] S1. Weigh 150 mg of hyaluronic acid and 300 mg of polyethyleneimine, add 30 ml of distilled water, stir to make it dissolve completely and become a transparent liquid;

[0039] S2. Put the above solution in a reaction kettle for hydrothermal reaction, the hydrothermal temperature is 180°C, and the reaction time is 6 h;

[0040] S3. Filtrating the liquid obtained by the hydrothermal method with a microporous filter membrane with a pore size of 0.22 μm to obtain an aqueous solution of nitrogen-doped carbon dots;

[0041] S4. Mix and stir 20 ml of the nitrogen-doped carbon dot aqueous solution obtained in S3 with 0.1 M, 20 ml of copper ions, and dialyze for 72 h with a cellulose dialysis bag with a molecular weight cut-off of 500-1000 to obtain targeted copper-doped carbon dots Aqueous solution of , after freeze-drying, solid targeted copper-doped carbon dots were obtained.

preparation Embodiment 3

[0043] The preparation of targeted copper-doped carbon dots comprises the following steps:

[0044] S1. Weigh 200 mg of hyaluronic acid and 400 mg of polyethyleneimine, add 40 ml of distilled water, stir to make it dissolve completely and become a transparent liquid;

[0045] S2. Put the above solution in a reaction kettle for hydrothermal reaction, the hydrothermal temperature is 180°C, and the reaction time is 6 h;

[0046] S3. Filtrating the liquid obtained by the hydrothermal method with a microporous filter membrane with a pore size of 0.22 μm to obtain an aqueous solution of nitrogen-doped carbon dots;

[0047] S4. Mix and stir 20 ml of the nitrogen-doped carbon dot aqueous solution obtained in S3 with 0.1 M, 20 ml of copper ions, and dialyze for 72 h with a cellulose dialysis bag with a molecular weight cut-off of 500-1000 to obtain targeted copper-doped carbon dots Aqueous solution of , after freeze-drying, solid targeted copper-doped carbon dots were obtained.

[00...

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Abstract

The invention discloses a targeted copper-doped carbon dot and its preparation method and application. The preparation method is to obtain nitrogen-doped carbon dots through a one-step hydrothermal method of hyaluronic acid and polyethyleneimine, and then simply mix them with copper ions Copper-doped carbon dots were obtained. The preparation method of the present invention is simple and convenient to operate, and the raw materials are safe and easy to obtain. The prepared copper-doped carbon dots have low cytotoxicity, good biocompatibility, good dispersibility in aqueous solution, good absorption in the near-infrared region, and Good photothermal conversion efficiency; has good photosensitization effect, generates active oxygen under the irradiation of near-infrared light; and can respond to reduced glutathione to restore fluorescence imaging. The copper-doped carbon dots prepared by the invention can target and respond to photothermal and photodynamic therapy mediated by fluorescence imaging of the tumor microenvironment, and have broad application prospects in the field of cancer treatment.

Description

technical field [0001] The invention relates to the field of biomedical materials, in particular to a targeted copper-doped carbon dot and its preparation method and application. Background technique [0002] Tumor phototherapy includes photothermal therapy (PTT) and photodynamic therapy (PDT). Photothermal therapy converts light energy into heat energy to ablate cancer cells through photothermal conversion agents. Photodynamic therapy means that photodynamic agents generate cytotoxic active oxygen under the irradiation of light, and then kill cancer cells. Due to its unique advantages such as high selectivity, negligible side effects and drug resistance, low systemic toxicity, and small wounds, phototherapy has become a promising new cancer treatment method. However, there are still some problems and deficiencies in this emerging research field. Traditional photothermal therapy conversion agents are mainly gold nanomaterials and carbon nanomaterials. These nanomaterials g...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K41/00A61K49/00A61P35/00
CPCA61K41/0052A61K41/0057A61K49/0067A61P35/00
Inventor 沈星灿张丽珍
Owner GUANGXI NORMAL UNIV
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