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Targeting copper-doped carbon dots and preparing method and application thereof

A nitrogen-doped carbon dot and copper-doped technology, applied in pharmaceutical formulations, photodynamic therapy, preparations for in vivo experiments, etc., can solve the problem that copper-based nanomaterials fail to reach cancer cells accurately and copper-based nanomaterials fail Targeted modification, photothermal, and photodynamic therapy have not been reported to achieve good photothermal conversion efficiency, good photosensitivity, and good targeting.

Active Publication Date: 2019-08-23
GUANGXI NORMAL UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] In recent years, the synthesis of multifunctional copper-based nanomaterials has made great progress, but most of the synthesized copper-based nanomaterials have not been targetedly modified, which makes it impossible for copper-based nanomaterials to accurately reach cancer cells during application. There is blindness in the cell location and phototherapy process. At the same time, the photothermal and photodynamic therapy mediated by copper-doped carbon dots in response to tumor microenvironment fluorescence imaging have not yet been reported.

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  • Targeting copper-doped carbon dots and preparing method and application thereof
  • Targeting copper-doped carbon dots and preparing method and application thereof
  • Targeting copper-doped carbon dots and preparing method and application thereof

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preparation Embodiment 1

[0031] The preparation of targeted copper-doped carbon dots comprises the following steps:

[0032] S1. Weigh 100 mg of hyaluronic acid and 200 mg of polyethyleneimine, add 20 ml of distilled water, stir to make it dissolve completely and become a transparent liquid;

[0033] S2. Put the above solution in a reaction kettle for hydrothermal reaction, the hydrothermal temperature is 180°C, and the reaction time is 6 h;

[0034] S3. Filtrating the liquid obtained by the hydrothermal method with a microporous membrane with a pore size of 220 μm to obtain an aqueous solution of nitrogen-doped carbon dots;

[0035] S4. Mix and stir 20 ml of the nitrogen-doped carbon dot aqueous solution obtained in S3 with 0.1 M, 20 ml of copper ions, and dialyze for 72 h with a cellulose dialysis bag with a molecular weight cut-off of 500-1000 to obtain targeted copper-doped carbon dots Aqueous solution of , after freeze-drying, solid targeted copper-doped carbon dots were obtained.

preparation Embodiment 2

[0037] The preparation of targeted copper-doped carbon dots comprises the following steps:

[0038] S1. Weigh 150 mg of hyaluronic acid and 300 mg of polyethyleneimine, add 30 ml of distilled water, stir to make it dissolve completely and become a transparent liquid;

[0039] S2. Put the above solution in a reaction kettle for hydrothermal reaction, the hydrothermal temperature is 180°C, and the reaction time is 6 h;

[0040] S3. Filtrating the liquid obtained by the hydrothermal method with a microporous membrane with a pore size of 220 μm to obtain an aqueous solution of nitrogen-doped carbon dots;

[0041] S4. Mix and stir 20 ml of the nitrogen-doped carbon dot aqueous solution obtained in S3 with 0.1 M, 20 ml of copper ions, and dialyze for 72 h with a cellulose dialysis bag with a molecular weight cut-off of 500-1000 to obtain targeted copper-doped carbon dots Aqueous solution of , after freeze-drying, solid targeted copper-doped carbon dots were obtained.

preparation Embodiment 3

[0043] The preparation of targeted copper-doped carbon dots comprises the following steps:

[0044] S1. Weigh 200 mg of hyaluronic acid and 400 mg of polyethyleneimine, add 40 ml of distilled water, stir to make it dissolve completely and become a transparent liquid;

[0045] S2. Put the above solution in a reaction kettle for hydrothermal reaction, the hydrothermal temperature is 180°C, and the reaction time is 6 h;

[0046] S3. Filtrating the liquid obtained by the hydrothermal method with a microporous membrane with a pore size of 220 μm to obtain an aqueous solution of nitrogen-doped carbon dots;

[0047] S4. Mix and stir 20 ml of the nitrogen-doped carbon dot aqueous solution obtained in S3 with 0.1 M, 20 ml of copper ions, and dialyze for 72 h with a cellulose dialysis bag with a molecular weight cut-off of 500-1000 to obtain targeted copper-doped carbon dots Aqueous solution of , after freeze-drying, solid targeted copper-doped carbon dots were obtained.

[0048] The ...

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Abstract

The invention discloses targeting copper-doped carbon dots and a preparing method and application thereof. The preparing method comprises the steps that hyaluronic acid and polyethylenimine are subjected to a one-step hydrothermal method to obtain nitrogen-doped carbon dots, and then simply mixing the nitrogen-doped carbon dots with copper ions to obtain the copper-doped carbon dots. The preparingmethod is easy and convenient to operate, raw materials are safe and easy to obtain, and the prepared copper-doped carbon dots have low cell toxicity, high biocompatibility and good dispersity in anaqueous solution, have good absorption performance in a near-infrared area, and have high photothermal conversion efficiency; the targeting copper-doped carbon dots have a good photosensitization effect, and active oxygen is generated under irradiation of near-infrared light; meanwhile, the targeting copper-doped carbon dots can respond to reduced glutathione, and restore fluorescence imaging. Theprepared copper-doped carbon dots can make a targeting response to photothermal and photodynamic therapy mediated by fluorescence imaging of a tumor microenvironment, and have a wide application prospect in the field of cancer therapy.

Description

technical field [0001] The invention relates to the field of biomedical materials, in particular to a targeted copper-doped carbon dot and its preparation method and application. Background technique [0002] Tumor phototherapy includes photothermal therapy (PTT) and photodynamic therapy (PDT). Photothermal therapy converts light energy into heat energy to ablate cancer cells through photothermal conversion agents. Photodynamic therapy means that photodynamic agents generate cytotoxic active oxygen under the irradiation of light, and then kill cancer cells. Due to its unique advantages such as high selectivity, negligible side effects and drug resistance, low systemic toxicity, and small wounds, phototherapy has become a promising new cancer treatment method. However, there are still some problems and deficiencies in this emerging research field. Traditional photothermal therapy conversion agents are mainly gold nanomaterials and carbon nanomaterials. These nanomaterials g...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K41/00A61K49/00A61P35/00
CPCA61K41/0052A61K41/0057A61K49/0067A61P35/00
Inventor 沈星灿张丽珍
Owner GUANGXI NORMAL UNIV
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